EMAGE mouse gene expression spatial database

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EMAGE:12725

Sirpa signal-regulatory protein alpha ( MGI:108563)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013621. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013621_01	euxassay_013621_02	euxassay_013621_03	euxassay_013621_04

euxassay_013621_05	euxassay_013621_06	euxassay_013621_07	euxassay_013621_08	euxassay_013621_09

euxassay_013621_10	euxassay_013621_11	euxassay_013621_12	euxassay_013621_13	euxassay_013621_14

euxassay_013621_15	euxassay_013621_16	euxassay_013621_17	euxassay_013621_18	euxassay_013621_19

euxassay_013621_20	euxassay_013621_21	euxassay_013621_22	euxassay_013621_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12725_wholemount_strong.wlz

12725_wholemount_moderate.wlz

12725_wholemount_weak.wlz

12725_wholemount_possible.wlz

12725_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12725_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hypothalamus mantle layer

moderate

regional

moderate expression: see section 14 15 weak expression: see section 09 10

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 14 weak expression: see section 06 07 08 09 10 11 13 15 16

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 18 19 20 21 22 23

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 14 weak expression: see section 12

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 15 16 weak expression: see section 09 10 11

medulla oblongata floor plate

strong

regional

strong expression: see section 11

metencephalon floor plate

strong

regional

strong expression: see section 11

midbrain floor plate

strong

regional

strong expression: see section 11 12

spinal cord floor plate

strong

regional

strong expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31964
Entity Detected:	Sirpa, signal-regulatory protein alpha ( MGI:108563)
Sequence:	sense strand is shown >T31964 AGCCCACATCACCTTGGATAGAAGCCCTCTTCGTGGGATTGCTAACCTGTCTAACTTCATCCGAGTTTCA CCCACCGTGAAGGTCACCCAACAGTCCCCGACGTCAATGAACCAGGTGAACCTCACCTGCCGGGCTGAGA GGTTCTACCCCGAGGATCTCCAGCTGATCTGGCTGGAGAATGGAAACGTATCACGGAATGACACGCCCAA GAATCTCACAAAGAACACGGATGGGACCTATAATTACACAAGCTTGTTCCTGGTGAACTCATCTGCTCAT AGAGAGGACGTGGTGTTCACGTGCCAGGTGAAGCACGACCAACAGCCAGCGATCACCCGAAACCATACCG TGCTGGGATTTGCCCACTCGAGTGATCAAGGGAGCATGCAAACCTTCCCTGATAATAATGCTACCCACAA CTGGAATGTCTTCATCGGTGTGGGCGTGGCGTGTGCTTTGCTCGTAGTCCTGCTGATGGCTGCTCTCTAC CTCCTCCGGATCAAACAGAAGAAAGCCAAGGGGTCAACATCTTCCACACGGTTGCACGAGCCCGAGAAGA ACGCCAGGGAAATAACCCAGATCCAGGACACAAATGACATCAACGACATCACATACGCAGACCTGAATCT GCCCAAAGAGAAGAAGCCCGCACCCCGGGCCCCTGAGCCTAACAACCACACAGAATATGCAAGCATTGAG ACAGGCAAAGTGCCTAGGCCAGAGGATACCCTCACCTATGCTGACCTGGACATGGTCCACCTCAGCCGGG CACAGCCAGCCCCCAAGCCTGAGCCATCTTTCTCAGAGTATGCTAGTGTCCAGGTCCAGAGGAAGTGAAT GGGGCTGTGGTCTGTACTAGGCCCCATCCCCACAAGTTTTCTTGTCCTACATGGAGTGGCCATGACGAGG ACATCCAGCCAGCCAATCCTGTCCCCAGAAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5368250), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60485. Forward Primer - name:060485_F_IRAV100_e10_Ptpns1, sequence:AGCCCACATCACCTTGGA; Reverse Primer - name:060485_R_SP6_IRAV100_e10_Ptpns1, sequence:GCCTTCTGGGGACAGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12723 same embryo
	EMAGE:12724 same embryo
	EMAGE:12726 same embryo
	EMAGE:12722 same embryo
	EurExpress:euxassay_013621 same experiment
	MGI:4828078 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source