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EMAGE:12802

Thpo thrombopoietin ( MGI:101875)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802
"Pseudo-wholemount" of euxassay_011564. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011564_01 euxassay_011564_02 euxassay_011564_03 euxassay_011564_04
EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802
euxassay_011564_05 euxassay_011564_06 euxassay_011564_07 euxassay_011564_08 euxassay_011564_09
EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802
euxassay_011564_10 euxassay_011564_11 euxassay_011564_12 euxassay_011564_13 euxassay_011564_14
EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802 EMAGE:12802
euxassay_011564_15 euxassay_011564_16 euxassay_011564_17 euxassay_011564_18 euxassay_011564_19
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12802Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12802_wholemount_strong.wlz
12802_wholemount_moderate.wlz
12802_wholemount_weak.wlz
12802_wholemount_possible.wlz
12802_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12802_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30323
Entity Detected:Thpo, thrombopoietin ( MGI:101875)
Sequence:sense strand is shown

>T30323
ACCTCTCTCCCACCCGACTCTGCCGAAAGAAGCACAGAAGCTCAAGCCGCCTCCATGGCCCCAGGAAAGA
TTCAGGGGAGAGGCCCCATACAGGGAGCCACTTCAGTTAGACACCCTGGCCAGAATGGAGCTGACTGATT
TGCTCCTGGCGGCCATGCTTCTTGCAGTGGCAAGACTAACTCTGTCCAGCCCCGTAGCTCCTGCCTGTGA
CCCCAGACTCCTAAATAAACTGCTGCGTGACTCCCACCTCCTTCACAGCCGACTGAGTCAGTGTCCCGAC
GTCGACCCTTTGTCTATCCCTGTTCTGCTGCCTGCTGTGGACTTTAGCCTGGGAGAATGGAAAACCCAGA
CGGAACAGAGCAAGGCACAGGACATTCTAGGGGCAGTGTCCCTTCTACTGGAGGGAGTGATGGCAGCACG
AGGACAGTTGGAACCCTCCTGCCTCTCATCCCTCCTGGGACAGCTTTCTGGGCAGGTTCGCCTCCTCTTG
GGGGCCCTGCAGGGCCTCCTAGGAACCCAGCTTCCTCTACAGGGCAGGACCACAGCTCACAAGGACCCCA
ATGCCCTCTTCTTGAGCTTGCAACAACTGCTTCGGGGAAAGGTGCGCTTCCTGCTTCTGGTAGAAGGTCC
CACCCTCTGTGTCAGACGGACCCTGCCAACCACAGCTGTCCCAAGCAGTACTTCTCAACTCCTCACACTA
AACAAGTTCCCAAACAGGACTTCTGGATTGTTGGAGACGAACTTCAGTGTCACAGCCAGAACTGCTGGCC
CTGGACTTCTGAGCAGGCTTCAGGGATTCAGAGTCAAGATTACTCCTGGTCAGCTAAATCAAACCTCCAG
GTCCCCAGTCCAAATCTCTGGATACCTGAACAGGACACACGGACCTGTGAATGGAACTCATGGGCTCTTT
GCTGGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3593885), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 11191. Forward Primer - name:011191_F_IRAV08-11_K18_Thpo, sequence:ACCTCTCTCCCACCCGAC; Reverse Primer - name:011191_R_SP6_IRAV08-11_K18_Thpo, sequence:GTTCCAGCAAAGAGCCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12801 same embryo
 EMAGE:12800 same embryo
 EMAGE:12799 same embryo
 EMAGE:12803 same embryo
 EurExpress:euxassay_011564 same experiment
 MGI:4828703 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS