EMAGE mouse gene expression spatial database

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EMAGE:12803

Reg4 regenerating islet-derived family, member 4 ( MGI:1914959)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011584. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011584_01	euxassay_011584_02	euxassay_011584_03	euxassay_011584_04

euxassay_011584_05	euxassay_011584_06	euxassay_011584_07	euxassay_011584_08	euxassay_011584_09

euxassay_011584_10	euxassay_011584_11	euxassay_011584_12	euxassay_011584_13	euxassay_011584_14

euxassay_011584_15	euxassay_011584_16	euxassay_011584_17	euxassay_011584_18	euxassay_011584_19

euxassay_011584_20	euxassay_011584_21	euxassay_011584_22	euxassay_011584_23	euxassay_011584_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12803_wholemount_strong.wlz

12803_wholemount_moderate.wlz

12803_wholemount_weak.wlz

12803_wholemount_possible.wlz

12803_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12803_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

radius

weak

regional

weak expression: see section 01

humerus

moderate

regional

moderate expression: see section 01 05 06 weak expression: see section 02

fibula

moderate

regional

moderate expression: see section 02

tibia

moderate

regional

moderate expression: see section 02

femur

moderate

regional

moderate expression: see section 03 07 08 09 10 22 23 weak expression: see section 04 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 03 10 11 12

axial skeleton

moderate

regional

moderate expression: see section 15 16 17

scapula

moderate

regional

moderate expression: see section 05 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31009
Entity Detected:	Reg4, regenerating islet-derived family, member 4 ( MGI:1914959)
Sequence:	sense strand is shown >T31009 AACTGGAACGGGCTCTGAGGGCCTTGAAATCAAAGCATTGAAGTATCATCTCCATCGAAAGAGGAAGATG GCTTCCAAAGGCGTGCGGCTACTCTTACTGCTGAGCTGGGTAGCTGGCCCCGAAGTCCTGAGCGATATCT TGAGACCCAGCTGTGCCCCAGGATGGTTTTACTATAGGTCCCACTGCTATGGGTACTTCCGGAAGCTAAG AAACTGGTCTCATGCTGAGCTGGAGTGTCAGTCATATGGAAATGGATCCCATCTGGCATCTGTCTTGAAT CAAAAGGAAGCCAGTGTCATATCAAAGTACATAACTGGCTATCAGAGAAACCTGCCTGTGTGGATTGGCC TGCATGACCCACAAAAGAAGCAATTATGGCAATGGACTGATGGGTCTACAAACCTGTACAGACGCTGGAA TCCCAGGACAAAGAGTGAAGCCAGGCATTGCGCTGAGATGAACCCCAAGGATAAATTCTTAACTTGGAAC AAAAATGGATGTGCCAACCGCCAACACTTCCTGTGCAAGTATAAGACATAGAGCAAGAATCAAGCATCTA CCAGCCTTGCACAAACTCTTCCCACTTCCCTCTCACCTGGTGGCTGATCTAATCATTATCCCAGAGTAAA CACTGTAGCAAACATTGAGGAGGCCTCCAGGGCACTGGCTATCAAGCCCCGCTTAGCATGGTGGGACAGT GGCTTCCGGTCTCAGAGTTTAGCATGGTGGGAGGGTGACTTCCGGTCTCAGAGATTAGCATGGTGGGACA AGGGCTTCTGGTCTCCGTGTTCACTCTACAATCCTTTCTGGTACTCCGCTTCCCTCTCATT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4216795), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22976. Forward Primer - name:022976_F_IRAV32-35_L09_Reg4, sequence:AACTGGAACGGGCTCTGA; Reverse Primer - name:022976_R_SP6_IRAV32-35_L09_Reg4, sequence:CAATGAGAGGGAAGCGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12801 same embryo
	EMAGE:12800 same embryo
	EMAGE:12802 same embryo
	EMAGE:12799 same embryo
	EurExpress:euxassay_011584 same experiment
	MGI:4827689 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source