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EMAGE:12853

Pdhb pyruvate dehydrogenase (lipoamide) beta ( MGI:1915513)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12853 EMAGE:12853 EMAGE:12853 EMAGE:12853 EMAGE:12853
"Pseudo-wholemount" of euxassay_011605. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011605_01 euxassay_011605_02 euxassay_011605_03 euxassay_011605_04
EMAGE:12853 EMAGE:12853 EMAGE:12853 EMAGE:12853 EMAGE:12853
euxassay_011605_05 euxassay_011605_06 euxassay_011605_07 euxassay_011605_08 euxassay_011605_09
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euxassay_011605_15 euxassay_011605_16 euxassay_011605_17 euxassay_011605_18 euxassay_011605_19
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euxassay_011605_20 euxassay_011605_21 euxassay_011605_22 euxassay_011605_23 euxassay_011605_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12853Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12853_wholemount_strong.wlz
12853_wholemount_moderate.wlz
12853_wholemount_weak.wlz
12853_wholemount_possible.wlz
12853_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12853_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31008
Entity Detected:Pdhb, pyruvate dehydrogenase (lipoamide) beta ( MGI:1915513)
Sequence:sense strand is shown

>T31008
GAGGACACGGGCAAGATGGCGGTGGTGGCTGGTCTGGTGCGGGGACCCTTGCGGCAGGCTTCCGGGCTGC
TGAAGAGGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAATCAAGG
TATGGATGAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGCCCAGTATGACGGT
GCATACAAGGTTAGCAGAGGCCTGTGGAAGAAATACGGTGACAAGAGGATCATCGACACCCCCATCTCAG
AGATGGGCTTTGCTGGAATTGCTGTTGGTGCAGCTATGGCTGGGTTGCGGCCTATCTGTGAATTTATGAC
CTTCAATTTCTCTATGCAAGCTATTGACCAGGTTATAAACTCAGCTGCCAAGACTTACTATATGTCCGCT
GGCCTTCAGCCTGTGCCCATAGTATTCAGGGGGCCCAATGGTGCCTCAGCAGGTGTAGCTGCTCAGCACT
CGCAATGCTTTGCTGCATGGTATGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGA
TGCAAAAGGACTTATTAAATCGGCCATTCGTGATAATAACCCAGTGGTAATGCTAGAAAATGAACTGATG
TATGGAGTTGCATTTGAACTTCCTGCAGAAGCTCAGTCAAAAGATTTCCTGATTCCTATTGGAAAAGCCA
AAATTGAAAGGCAAGGGACCCACATCACTGTAGTTGCCCATTCAAGACCAGTGGGCCACTGCCTAGAAGC
TGCAGCTGTATTGTCTAAGGAGGGAATTGAATGTGAGGTAATAAATCTGCGCACCATCAGACCAAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4210204), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35664. Forward Primer - name:035664_F_IRAV32-35_J17_Pdhb, sequence:GAGGACACGGGCAAGATG; Reverse Primer - name:035664_R_SP6_IRAV32-35_J17_Pdhb, sequence:CATTGGTCTGATGGTGCG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12852 same embryo
 EMAGE:12851 same embryo
 EMAGE:12849 same embryo
 EMAGE:12850 same embryo
 EurExpress:euxassay_011605 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS