EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12874

Tctn3 tectonic family member 3 ( MGI:1914840)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011590. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011590_01	euxassay_011590_02	euxassay_011590_03	euxassay_011590_04

euxassay_011590_05	euxassay_011590_06	euxassay_011590_07	euxassay_011590_08	euxassay_011590_09

euxassay_011590_10	euxassay_011590_11	euxassay_011590_12	euxassay_011590_13	euxassay_011590_14

euxassay_011590_15	euxassay_011590_16	euxassay_011590_17	euxassay_011590_18	euxassay_011590_19

euxassay_011590_20	euxassay_011590_21	euxassay_011590_22	euxassay_011590_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12874_wholemount_strong.wlz

12874_wholemount_moderate.wlz

12874_wholemount_weak.wlz

12874_wholemount_possible.wlz

12874_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12874_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 weak expression: see section 13

diencephalon roof plate

weak

regional

weak expression: see section 13

choroid invagination

moderate

regional

moderate expression: see section 06 07 08 09 10 15 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 weak expression: see section 13

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32019
Entity Detected:	Tctn3, tectonic family member 3 ( MGI:1914840)
Sequence:	sense strand is shown >T32019 GGAGCTGGGGGACTTTGTGCCGAGAGCAACCCTGCAGGTTTCCTAGAGAGTAAAAGCACAACGTGTCCTC GTTTCTTCAGGGACCTGGCCAGTAGCTGCACCTCGGAGCCAGCCCTGGATGCTGCCTCTTACTATAACTT CAGAGTCTTGAAGGTTCCAAGAGGTGTGACTGATCTTCAGAATATGAAGTTCCAGGTTCCTGTGACGCTT GCCTCACAAGCCAGCCCTCCTCTGTTGGCTGGAAACACATGTCAGAATATCGTTTCCCAGGTCGTCTACG AGATAGAGACCAATGGGACCTTTGGGATCCAGAAAGTCTCTGTCAGCTTCCGACAGACCAACCTGACTGT CAAGCCAGGGGTCTCCTTACAGCAAGACTTCATCGTTCACTTCAGGGCATTTCAACAGAGAAAGGCCGCT GCTCCTGCTGCTCCGAGAAGTGGGAATCCTGGCTACCTCACTGGGAAGCCACTCTTAGTGCTAACTGGTG ACACAAGTCACTCGATGACCCTGTTGCAGAGCGAGGGTAATGGGCTTTGCTCTGCTAAGAGACACGCAGT ACAGTTTGGAGTGAACGCAGTATCTGGGTGCCAGTTAAGACCCAGAGAGGTGAACTGCAGCCACTTGCAG GAGGAGGTTTATCAGACTCTGCACGGAAGGCCCAGACCGGAGCACGTTGCCGTCTTTGGCAACGCTGACC CAGCCCAGAGAGGAGGGTGGACCAGGATCCTCAGCAGAGACTGCAGCGTTTCAGCAGTTAACTGCACTTC CTGCTGTCTGATACCAGTGTCCCTGGAGATCCAGGTGTTGTGGGCACATCTAGGACTTCAGTCCAACCCA CAAGCTCATGTGGCCGGAGCCAGATTCCTGTACCAATGCAAGTCTGTACAGGAGCACCAGAGAGGAATAG AAGTATCTTTGACGACTCTTGTGAACTTTGTGGACATTACCCAGAAGCCAGAGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3710046), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21756. Forward Primer - name:021756_F_IRAV51-54_P07_4930521E07Rik, sequence:GGAGCTGGGGGACTTTGT; Reverse Primer - name:021756_R_SP6_IRAV51-54_P07_4930521E07Rik, sequence:AGGCTCTGGCTTCTGGGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12872 same embryo
	EMAGE:12876 same embryo
	EMAGE:12873 same embryo
	EMAGE:12875 same embryo
	EMAGE:12871 same embryo
	EurExpress:euxassay_011590 same experiment
	MGI:4828651 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source