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EMAGE:12939

Rnmt RNA (guanine-7-) methyltransferase ( MGI:1915147)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939
"Pseudo-wholemount" of euxassay_013666. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013666_01 euxassay_013666_02 euxassay_013666_03 euxassay_013666_04
EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939
euxassay_013666_05 euxassay_013666_06 euxassay_013666_07 euxassay_013666_08 euxassay_013666_09
EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939
euxassay_013666_10 euxassay_013666_11 euxassay_013666_12 euxassay_013666_13 euxassay_013666_14
EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939
euxassay_013666_15 euxassay_013666_16 euxassay_013666_17 euxassay_013666_18 euxassay_013666_19
EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939 EMAGE:12939
euxassay_013666_20 euxassay_013666_21 euxassay_013666_22 euxassay_013666_23 euxassay_013666_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12939Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12939_wholemount_strong.wlz
12939_wholemount_moderate.wlz
12939_wholemount_weak.wlz
12939_wholemount_possible.wlz
12939_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12939_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
weak weak
regionalweak expression: see section 04 05 19 20 21
facial vii ganglion
weak weak
regionalweak expression: see section 02 03 18 19
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 05 16 17
trigeminal v ganglion
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 16 17 18 19
vagus x ganglion
weak weak
regionalweak expression: see section 06 07
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 05 16 17
dorsal root ganglion
weak weak
regionalweak expression: see section 06 07 08 09 10 11 15 16 17
mandible
weak weak
regionalweak expression: see section 02 03 05 06 07 15 17 18
lower jaw incisor
weak weak
regionalweak expression: see section 10 11 14 15
lower jaw molar
weak weak
regionalweak expression: see section 05 18
maxilla
weak weak
regionalweak expression: see section 07
upper jaw incisor
weak weak
regionalweak expression: see section 10 14 15
upper jaw molar
weak weak
regionalweak expression: see section 05 18
metanephros
weak weak
regionalweak expression: see section 06 07 08 09 10 16 17 18 19
testis
weak weak
regionalweak expression: see section 06 07 18 19 20 21
urethra of male
weak weak
regionalweak expression: see section 13 14
lung
moderate moderate
regionalmoderate expression: see section 07 17 18 19 21 22 weak expression: see section 05 06 08 09 10 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32008
Entity Detected:Rnmt, RNA (guanine-7-) methyltransferase ( MGI:1915147)
Sequence:sense strand is shown

>T32008
AACTGCAGCCTCAGGACGATGAGGTACCGCAGAAGTTGCAGAAGCTGGAGGAAGGACACAGCTCAGCCGT
GGCTGCCCACTATAATGAGCTTCAGGAAGTTGGCCTGGCGAAGCGTAGTCAAAGTCGCATTTTTTACCTA
AGAAACTTCAATAATTGGATTAAAAGTATTCTGATTGGGGAAATTTTAGAAAAGGTACGACAAAGAAAAA
CCCGTGACATCACTGTTTTGGACCTGGGATGTGGAAAAGGTGGAGATTTGCTCAAGTGGAGGAAGGGGAG
AATTAGCAGGCTAGTGTGTGCTGATATCGCTGATATTTCTATGAAACAATGTCAACAGCGTTACGAAGAC
ATGAGATGTCGTCGCGATAATGAGCATATTTTTAGTGCAGAATTTATAACTGCTGACTGTTCAAAGGAAC
TTTTGGTTGAAAAATTCCGTGACCCAGAAATGTACTTTGATGTCTGCAGCTGCCAGTTTGCTTGCCATTA
CTCCTTTGAGTCCCAGGTGCAGGCGGATACGATGCTTAGAAATGCCTGCGGGAGGCTTAACCCTGGCGGC
TATTTTATCGGCACCACTCCCAATAGCTTTGAACTGATAAGACGCCTTGAAGCTTCAGAAACAGAATCAT
TTGGAAATGAAATATACACTGTGAAATTCCAGAAGAAAGGAAATTATCCTTTATTTGGCTGCAAATATGA
CTTTAACTTGGAAGGTGTTGTGGATGTCCCTGAATTCTTGGTCTATTTTCCATTGCTAACTGAAATGGCA
AAGAAGTATAATATGAAACTAATCTACAAAAAAACATTTTTGGAATTCTATGAAGAAAAGATTAAAAACA
ATGAAAACAAAATGCTCTTAAAACGAATGCAGGCCCTGGAGCAATACCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4208274), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22896. Forward Primer - name:022896_F_IRAV32-35_J23_Rnmt, sequence:AACTGCAGCCTCAGGACG; Reverse Primer - name:022896_R_SP6_IRAV32-35_J23_Rnmt, sequence:AGGGTATTGCTCCAGGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12942 same embryo
 EMAGE:12940 same embryo
 EMAGE:12938 same embryo
 EMAGE:12941 same embryo
 EurExpress:euxassay_013666 same experiment
 MGI:4827782 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS