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EMAGE:12940

Mtif2 mitochondrial translational initiation factor 2 ( MGI:1924034)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940
"Pseudo-wholemount" of euxassay_013660. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013660_01 euxassay_013660_02 euxassay_013660_03 euxassay_013660_04
EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940
euxassay_013660_05 euxassay_013660_06 euxassay_013660_07 euxassay_013660_08 euxassay_013660_09
EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940
euxassay_013660_10 euxassay_013660_11 euxassay_013660_12 euxassay_013660_13 euxassay_013660_14
EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940
euxassay_013660_15 euxassay_013660_16 euxassay_013660_17 euxassay_013660_18 euxassay_013660_19
EMAGE:12940 EMAGE:12940 EMAGE:12940 EMAGE:12940
euxassay_013660_20 euxassay_013660_21 euxassay_013660_22 euxassay_013660_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12940Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12940_wholemount_strong.wlz
12940_wholemount_moderate.wlz
12940_wholemount_weak.wlz
12940_wholemount_possible.wlz
12940_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12940_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
weak weak
regionalweak expression: see section 04 05 20 21
liver lobe
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
testis
weak weak
regionalweak expression: see section 05 06 07 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32002
Entity Detected:Mtif2, mitochondrial translational initiation factor 2 ( MGI:1924034)
Sequence:sense strand is shown

>T32002
AACCTGATGGCTTTGGCAGAAGCAACAATTGCTCTCGCAGAAATCTTGGAACTGAAAGCAGATCCCACCG
GTCCAGTGGAAGGAACAGTAATAGAGTCTTTCACAGACAAAGGAAGAGGTCCTGTTACAACAGCTATAAT
TCAAAGAGGAACTCTGAGAAAAGGCTCAATTCTAGTTGCTGGGAAGAGTTGGGCAAAAGTTCGACTAATA
TTTGATGAAAATGGAAAAATACTTAATGAGGCCTATCCCAGCATGCCAGTGGGAATCATAGGCTGGAGAG
ACCTTCCTTCTGCAGGAGATGAAATTCTTGAAGTAGAATCTGAGCCAAGGGCCCGTGAAGTTATTGAATG
GAGGAAGTCTGAGCAAAAAGAAGAAAAAGGCAAAGATGACCTGAAAATAATGGAAGAAAAGCGAAGGGAA
CACCAAGAAGCGCATCGGAAAGCCCGTGAGAAGTATGGCAGTCTGCACTGGAAAGAGAGATCATATATAA
AGTTCCTTGAAAGGAAACAACAGAGACCCTTAAAGCCCAAAGAAAAGGTAGAAAGGCAATCAAATGTACT
TCCTATAATTATTAAAGGTGACGTTGATGGGTCTGTGGAGGCCATCTTGAACCTTCTGGATACCTACGAT
GCTTCCCACGAATGTGAACTCGAATTAGTACATTTTGGATTGGGTGACATCAGTGAAAATGATGTTACCT
TTGCTGAGACATTTGATGGTGTTATTTATGGTTTTAATGTGGAGGCAGGCAGTGCTATTCAGCAGTCAGC
TGCACAAAAGGGAGTTAAGATTAAACTTCACAAAATCATCTACCATCTTATTGAAGATTTGCAGGAGGAA
CTAAGCAGCAGATTGCCCCACACACTGGAGGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3585359), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21490. Forward Primer - name:021490_F_IRAV32-35_G13_Mtif2, sequence:AACCTGATGGCTTTGGCA; Reverse Primer - name:021490_R_SP6_IRAV32-35_G13_Mtif2, sequence:CTCCTCCAGTGTGTGGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12942 same embryo
 EMAGE:12939 same embryo
 EMAGE:12938 same embryo
 EMAGE:12941 same embryo
 EurExpress:euxassay_013660 same experiment
 MGI:4826494 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS