EMAGE mouse gene expression spatial database

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EMAGE:12984

Rnpc3 RNA-binding region (RNP1, RRM) containing 3 ( MGI:1914475)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013667. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013667_01	euxassay_013667_02	euxassay_013667_03	euxassay_013667_04

euxassay_013667_05	euxassay_013667_06	euxassay_013667_07	euxassay_013667_08	euxassay_013667_09

euxassay_013667_10	euxassay_013667_11	euxassay_013667_12	euxassay_013667_13	euxassay_013667_14

euxassay_013667_15	euxassay_013667_16	euxassay_013667_17	euxassay_013667_18	euxassay_013667_19

euxassay_013667_20	euxassay_013667_21	euxassay_013667_22	euxassay_013667_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12984_wholemount_strong.wlz

12984_wholemount_moderate.wlz

12984_wholemount_weak.wlz

12984_wholemount_possible.wlz

12984_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12984_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 06 08 09 10 11 12 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 10 15 16 17 18

pons mantle layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21

pons marginal layer

weak

regional

weak expression: see section 17

midbrain mantle layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 10

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 19 20 21

trigeminal v nerve

moderate

regional

moderate expression: see section 10 18

ventral grey horn

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 15 16 17 18 19 weak expression: see section 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 13 15 16 17 18

vomeronasal organ

weak

regional

weak expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30998
Entity Detected:	Rnpc3, RNA-binding region (RNP1, RRM) containing 3 ( MGI:1914475)
Sequence:	sense strand is shown >T30998 TCAAGCACAATCCTCGCAAATATAGTAAATGCTCTGGCAAGTGTCCCAAAATTCTATGTACAGGTGCTTC ATCTTATGAATAAAATGAATTTGCCCACACCATTTGGACCGATTACTGCACGACCTCCTATGTATGAGGA CTATATGCCATTGCATGCACCCCTTCCACCTACATCCCCTCAACCGCCTGAGGAGCCTCCCTTACCAGAT GAGGATGAGGATTTATCTAGCAAAGAATCAGAATATGAAAGCAGTGATGAGGAGGACCGACAGAGAATGA ACAGATTAATGGAACTAGCAAATCTTCAGCCCAAAAGACCCAAAACAGAAAAGCCACGCCACGTGAGAAA AAAGCGAAAAATAAAGGATATGTTGAATATACCTTCATCAGCTTCACATAGTTTGCATCCTGTGCTGTTA CCTTCGGATGTATTTGACCAGCCACAATCTGTAGGTAATAAAAAAATTGAGTTCAATATATCAACCAACA TGCCTGCAGCATTTAACAAAGATTTGGAGACACAACCAAATAATGAAGAAGAAAATTCTGATTCACCAGA CACTGGACTTGATTCAAATACAGGATTTGGCAAAATCTTCCCTAAACCTAATTTGAACATCACAGAAGAG ATTACAGAAGACTCTGATGAAATACCTTCACAATTTATTTCTAGAAAAGAGTTGGAAAAGGGTCGTATTT CTAGAGAAGAAATGGAAACACTTTCAGTTTTTAGAAGTTATGAACCTGGTGAACCAAACTGCAGAATTTA TGTAAAGAATTTAGCTAGACATGTTCAGGAAAAGGACCTTAAATTTATATTTGGAAGATACGTTGACTTT TCTTCAGAAACACAGCGGATCATGTTTGATATTCGCTTAATGAAAGAAGGACGCATGAAAGGACAAGCTT TTGTTGGACTTCCAAATGAAAAAGCAGCCGCTAAAGCGTTGAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3587176), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21510. Forward Primer - name:021510_F_IRAV32-35_G07_2810441O16Rik, sequence:TCAAGCACAATCCTCGCA; Reverse Primer - name:021510_R_SP6_IRAV32-35_G07_2810441O16Rik, sequence:TTTCAACGCTTTAGCGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12985 same embryo
	EMAGE:12986 same embryo
	EMAGE:12988 same embryo
	EMAGE:12989 same embryo
	EMAGE:12987 same embryo
	EurExpress:euxassay_013667 same experiment
	MGI:4827784 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source