EMAGE mouse gene expression spatial database

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EMAGE:12993

Lmod2 leiomodin 2 (cardiac) ( MGI:2135672)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013698. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013698_01	euxassay_013698_02	euxassay_013698_03	euxassay_013698_04

euxassay_013698_05	euxassay_013698_06	euxassay_013698_07	euxassay_013698_08	euxassay_013698_09

euxassay_013698_10	euxassay_013698_11	euxassay_013698_12	euxassay_013698_13	euxassay_013698_14

euxassay_013698_15	euxassay_013698_16	euxassay_013698_17	euxassay_013698_18	euxassay_013698_19

euxassay_013698_20	euxassay_013698_21	euxassay_013698_22	euxassay_013698_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12993_wholemount_strong.wlz

12993_wholemount_moderate.wlz

12993_wholemount_weak.wlz

12993_wholemount_possible.wlz

12993_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12993_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 09 10 11 13 14 15 17

midbrain mantle layer

moderate

regional

moderate expression: see section 07 09 14 15 17 weak expression: see section 10

ventral grey horn

weak

regional

weak expression: see section 10 11 13

heart ventricle

weak

regional

weak expression: see section 10 11 12 13 14 15

tongue muscle

moderate

regional

moderate expression: see section 11 12 13 14 weak expression: see section 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37782
Entity Detected:	Lmod2, leiomodin 2 (cardiac) ( MGI:2135672)
Sequence:	sense strand is shown >T37782 TTTGGCTACAGAAGGGGACTTAGTAAATATGAATCCATTGATGAGGATGAACTTCTGGCCTCCCTCTCAC CTGAAGAGCTGAAGGAGCTTGAGAGGGAGCTGGAAGACATTGAACCTGACCGAAACCTTCCTGTGGGGCT AAGGCAAAAGAGTCTGACAGAGAAAACCCCAACAGGAAACTTCAGCCGAGAGGCGCTGATGGCGTATTGG GAAAAGGAGTCCCAAAAACTTTTGGAGAAGGAACGGCTGGGGGAATGTGGAAAGGTTGCAGAAGAAGACA AGGAAGAGAGTGAAGAGGAACTAATTTTCACAGAGAGCAACAGCGAAGTCTCTGAGGAGGTGTGTACAGA AGATGAGGAAGAGTCTCAGGAGGAAGAAGAGGATAGCGAGGAAGAAGAGGACAGTGAGGAAGAGGAAGAG ACAACAGAAGCCACAAAACATATTAATGGAACTGTAAGCTACAATAGTGTTAATACTGATAACTCTAAGC CAAAGACATTTAAAAGCCAAATAGAAAATATAAATTTAACCAACGGCAACAGTGGAAGAACACAGAGGAA CTCCGAGTCACCAGCCGCCATTCACCCTTGTGGAAATCCCACTGTTATTGAGGATGCTCTGGAAAAGATT AGAAACAATGACCCTGATACGACAGAAGTTAATCTGAACAATATAGAGAACATCACCACCCAGACCTTAT CCCGATTTGCTGAAGCTCTCAAGGAGAACACAGTGGTGAAGACGTTCAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79268. Forward Primer - name:079268_F_cDNA_Lmod2, sequence:TTTGGCTACAGAAGGGGACTTA; Reverse Primer - name:079268_N_SP6_cDNA_Lmod2, sequence:GCTGAACGTCTTCACCACTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12995 same embryo
	EMAGE:12991 same embryo
	EMAGE:12994 same embryo
	EMAGE:12990 same embryo
	EMAGE:12992 same embryo
	EurExpress:euxassay_013698 same experiment
	MGI:4825952 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source