EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13130

Wdr48 WD repeat domain 48 ( MGI:1914811)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017339. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017339_01	euxassay_017339_02	euxassay_017339_03	euxassay_017339_04

euxassay_017339_05	euxassay_017339_06	euxassay_017339_07	euxassay_017339_08	euxassay_017339_09

euxassay_017339_10	euxassay_017339_11	euxassay_017339_12	euxassay_017339_13	euxassay_017339_14

euxassay_017339_15	euxassay_017339_16	euxassay_017339_17	euxassay_017339_18	euxassay_017339_19

euxassay_017339_20	euxassay_017339_21	euxassay_017339_22	euxassay_017339_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13130_wholemount_strong.wlz

13130_wholemount_moderate.wlz

13130_wholemount_weak.wlz

13130_wholemount_possible.wlz

13130_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13130_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9249
Entity Detected:	Wdr48, WD repeat domain 48 ( MGI:1914811)
Sequence:	sense strand is shown >T9249 CGGAGTGTCAACATGCAAGATGGCGGCCCATCACCGGCAGAACACAGCCGGGCGGAGGAAAGTGCAGGTT TCTTATGTAATTCGAGATGAAGTGGAGAAATACAATCGGAATGGAGTCAATGCCCTGCAACTGGACCCAG CACTCAACAGACTCTTCACAGCAGGTCGAGACTCCATCATAAGAATATGGAGTGTCAATCAGCACAAGCA AGATCCGTACATAGCATCTATGGAGCACCATACAGACTGGGTAAATGATGTCGTGCTCTGTTGCAATGGG AAAACGCTAATTTCTGCTTCTTCAGACACAACAGTCAAAGTCTGGAATGCACATAAAGGGTTCTGCATGT CCACACTCAGGACGCATAAGGACTATGTAAAGGCCTTAGCGTACGCCAAGGATAAAGAGCTGGTAGCGTC AGCTGGGCTGGACAGACAGATATTCCTTTGGGATGTGAACACTCTGACGGCACTGACTGCCTCAAACAAC ACCGTTACAACTTCTTCTTTAAGTGGGAACAAAGACTCCATTTATAGCCTGGCCATGAACCAACTGGGAA CAATCATTGTATCAGGGTCCACTGAGAAGGTTCTGAGAGTGTGGGATCCAAGAACCTGCGCGAAACTCAT GAAGCTGAAAGGACATACGGACAATGTGAAGGCCTTGCTGCTGCACAGGGACGGCACACAGTGCCTCTCT GGGAGTTCTGACGGGACCATTCGCCTGTGGTCCCTTGGCCAGCAGAGATGTATAGCAACATACCGAGTCC ATGATGAAGGTGTTTGGGCCCTACAGGTCAACGATGCCTTTACACATGTATATTCTGGAGGACGAGACAG GAAAATCTACTGCACAGACCTAAGGAACCCTGACATTCGGGTACTGATTTGTGAAGAAAAAGCACCAGTT CTCAAGATGGAACTTGACAGATCAGCAGATCCCCCTCCTGCAATCTGGGTTGCAACAACAAAGTCCACAG TAAATAAGTGGACACTGAAGGGAATTCATAATTTTCGAGCCTCTGGTGATTATGACAATGATTGTACAAA CCCCATAACGCCCCTCTGCACACAGCCCGACCAAGTTATTAAAGGGGGCGCTAGTATTATTCAGTGCCAC ATCCTAAATGACAAGCGACACATATTAACCAAAGATACGAATAACAATGTGGCGTACTGGGATGTATTGA AGGCATGCAAAGTGGAAGATTTGGGCAAAGTGGACTTTGAAGATGAAATTAAGAAAAGATTTAAAATGGT GTATGTTCCAAATTGGTTCTCAGTAGACCTAAAAACAGGGATGCTGACCATTACCCTGGATGAGAGTGAC TGCTTCGCTGCCTGGGTTTCTGCGAAAGATGCTGGCTTTAGCAGTCCTGATGGGTCAGACCCAAAACTGA ACTTAGGAGGACTTCTACTACAGGCACTGCTGGAATACTGGCCCAGGACACATGTGACTCCCATGGATGA AGAAGAGAATGAAGTGAACCATGTGAGTGGGGGACAGGAGAGCAGAGTCCAGAAGGGAAATGGTTATTTC CAAGTGCCCCCACACACCCCGGTGATCTTTGGAGAAGCTGGAGGTCGCACACTATTCAGGCTGCTCTGCC GGGACTCCGGGGGTGAGACTGAGGCGATGCTTCTCAATGAGACGGTGCCACAATGGGTGATTGACATCAC TGTGGATAAAAATATGCCCAAATTCAACAAAATTCCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCA AAAACTTTAAAAAAAGACAGGCTGTCTGCCAGTGACATGCTGCAGGTCCGGAAGGTGATGGAGCACGTCT ACGAGAAGATTATCAACCTGGACAATGAGTCCCAAACCACCAGCTCTTCTAATAATGAAAAGCCAGAGCA GGAGAAGGAGGAGGACATCGCGGTATTGGCAGAGGAGAAAATTGAACTTCTGTGCCAGGACCAGGTTTTG GACCCAAATATGGACCTTCGGACAGTAAAGCACTTCATATGGAAGAGTGGTGGGGACCTCACCCTCCATT ACCGTCAGAAGTCCACGTGAGGGTGGGCCCATGCTCCTGGGTTCTTCACCAACCACCTTTCTCTGTGGCC CCAAGAGTAGTCCTAGGAAGCCCACCACGTCCTACGGGAGCAAGACCTCCAACCGGCCTATTTGGTGACG TGACTAATGGAGATGTAATATAGAAACCAGGCTCCATGCATAGATTAAGGTGTCCCCAGGGACTCAAGAG TGCAAGGACAAGCCCCTGGCACTGTCCGGGGAGATGTACAGAGAATCTACAACAGGCCAGTGCAGACTTC GCTGACTCCTTTTGAAGGACCCCCCTTTGTCTCTTAGCACTTGTTAGAGAACATCAGCGGGGCCACATCA GTCGCCATGTTTCAGTTTCAAACCCACAGGCTCTGCAGCTTTTCCAAGTACATTCCACTCATGCAGTACC TATGAAGGCTTTTTCCAAATTTGTCATTATATAAAAAAAAACAAGTTGTTTTCACTATGTAAGAGTTATT TTTAATGGAAATCACTGTTGCACAATTCAAGAGCCAGCCCATTTCCTGAGAAATGACCATGTTGGGCTAC TTTTGAAATGCCACGTCTCAGCTGTGCTCTTGTAGGAAGACCTGCAACTCCAGGGCTGGAGCCTGCGCAG CACGTGGACCTGCAGGCTGCCACCCACAGCCTGTGCTGCGCAGTGCTGCCCATGGCGCCCTGGCTGTCCG ATAGCCCAAGCTGCTGTGGCCACTCCTTCCGCCAGTCTTCCTCGCGGGCAAAGCCAGCGAAGCCACAGCT TCCTGCTCCGCGTGAGCAGCACGACAACTGTCAGAACTTACGGCCATGCTGAAGTTGCAGTTTCATTCAG ATGCTACCTAAAGTTTATGTTACTAGAGTAAAAAGATCTCACAGTGAGTTGGTTTGATATTTATATTGTG GTTCATGTTTGTTTTTCCAGTTTTATACACGGGCTTTTGTTACGCCTTCGTCCAGGTCTTTCTGCCGTCT TTCTAACAGTCAAAAGCTTTCATAGACTCACTTGGATCTTGTACAGAATATTAACTTATTGGGGATAAAC ACTTCAACTTTTGGCAAAAACTACTTTGGATTCTTCCCAAGGTCATTTACATTCAGACAAATGGTCCAGC CCGTGAAGCTGCAGGAGATGAACCTTTGAGCACAGCGGAAGCACACGTGCCTTGGGGGTGGGGGGTGCAG CTTTCCCCATCCGTCCGTCTGCTCCTGCCTTGTGTGTGTGGTCCCTGCCCACAGGAGCACAGGGGAGGGG GGCTTGGGGAAGAAAGATGTTTTCTCTCTATCTCACAGGTCCTGCAGGGAACAGCTGAGGGGCCCAGATC TGTCCCTTACAAATTGACTGCAGAACAGGGTCCCCTCACTTAACTCTGGAACACAGACAGGCCATGGCTT CCATACCCTCAAGTGAGCAAGAACACTTGCCAGTGTTAAACCATTCTCCGCCCTAGAGTAGCCAGCTTTT CTACAGAAGTCCTTTCTATGCTGGACACATCGGTGCACTTTTGGTGCATATTTTTTAGTGATAGCTATAA AGTAAAAGCAGGGGGTGGGGTGGTCGTGTTTGCAGGAGGGGCCTCTGCACTAAGCGTAGGGTTTGCTGGT TGGTTCACTGGCGCAGTACTGATCCACAGGCAGTTGTGCCCCTATGGAGGAGTTTACCCCAGCATGGTTG TAATTTTTTCCCCAGCTAGTCAAAAATCAGTACTAAGTTTCCAGAAAACTGGACAGTCTGCAACGCCAAA GGGGCAGAGTCTGTATCTCGGGTTGTATAGAATAAAGCTTTAGTTACAAACAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6835444 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6835444 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13134 same embryo
	EMAGE:13133 same embryo
	EMAGE:13132 same embryo
	EMAGE:13129 same embryo
	EMAGE:13131 same embryo
	EurExpress:euxassay_017339 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source