EMAGE mouse gene expression spatial database

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EMAGE:13134

Ccdc116 coiled-coil domain containing 116 ( MGI:1924122)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017309. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017309_01	euxassay_017309_02	euxassay_017309_03	euxassay_017309_04

euxassay_017309_05	euxassay_017309_06	euxassay_017309_07	euxassay_017309_08	euxassay_017309_09

euxassay_017309_10	euxassay_017309_11	euxassay_017309_12	euxassay_017309_13	euxassay_017309_14

euxassay_017309_15	euxassay_017309_16	euxassay_017309_17	euxassay_017309_18	euxassay_017309_19

euxassay_017309_20	euxassay_017309_21	euxassay_017309_22	euxassay_017309_23	euxassay_017309_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13134_wholemount_strong.wlz

13134_wholemount_moderate.wlz

13134_wholemount_weak.wlz

13134_wholemount_possible.wlz

13134_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13134_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9252
Entity Detected:	Ccdc116, coiled-coil domain containing 116 ( MGI:1924122)
Sequence:	sense strand is shown >T9252 CGGCACGAGGCGGGCAGAGCGCGAGAGCGATCACCGGCTGCCACCAAGTGGGAGCCCCTAAACCAGCCGG GTCCTGTAGGGGACCGCCCTCACCTGCGGGCCAGCTCGGCCGCACTCTCTGCGGCTGTGCCGTTAACCAG CAGTGACACGCTGGTCACAGTTCCTGCCAGGAAGGCCTCTACGATGCCCTCATCGCGCCGCGGGCAATAA CCAAAGTCATCAGCGGTGACAACCAGCCGCACGCGGGGAAAAGCCATGGCTGCCAAACCCTGGCTGGAGA GCAGAGGGAATAGGTTGGAGCATCCAGACCCCGCCCTCGAACGCTGCCGAGGCCCCACCCCGACGCACAC GCCTAAATGCGGCTGCAAGCCGCCATTGTTCTCGCCACCTAGAGAGGAGTCTGCATCTGTGCTGTGGGAC CTGAAAAAGGAAGCAGAAAACAAATTTAATTATCCTCATCATGGCGAGGTGCCGCCACCACTCAGGATAT CTGGCTGACGACGAGGCTGCTCACAGCACCTATGTGGCCCCGCTGCCCAAGAAGCACCTGTTGCCAGAAA TGAGGCCAACCTGCAAGCTTGGGCGTGTACCACACCTACCATCAATGAACCAATACTCAGAGCACCAAAG CCACCAGCAAAACTTTAGGCACCCTCTGGCATTTGGTGGCTTCCTGGATTTCCTCACGGAGGGTCAGGTA CTGGACAGCTTGCAGACAGTTGTGGAGCAGGCAACAGAACGTTTGGCTGCCATGAAGACAGAGGCTGGCG TGCCACTGGTGGATATTCAAGACCCCGTGGAGGTGCCAAGTAGTAGGCATCGATCTCGAGCCAGACCCAG CATCGACACTGTGCACCGGCACCGTGCTCGGCCCACCCTGTGTGCTGGATGCCCAAACAATTACCCATCT TGCTCCAGTTCCATGTCTGATTCCCATAGCAGCATCACAGCTGGATGGCTGGGGTCCCACAGCCAAGACA GTGACCTGGGTGCCAGAGGCATAGGCTCACTGCCACCTATGAGGGACAAACTCCTGCTTGAGAAAAACCT CAAGAGGCTACTCCGACTGGAAAACAAAGGGAAAATCCTGAATCAATCCTGCTCCCAGAGGGATTCCCTG CTGTGGGACTCACTGGGCAGCCAAACCAGCAGTCAGTGGACCCGGGAGCAGCCCCTGTCTTGGTTCTCTG GGCTATTGGGCTCCAGTCCAGCCACCCCTGAAACTTCAGAGTTGGGACTTGGAGAACAGGAGATGATTTT CCTCAAACAAAAGTTAAACAAGGAGATGAAGTCACTGCTGAACCAGCCAAGACCCTTCAACCTGCCTACC TATTGTCCACTCAGGGAACCCCATCATACCCTGGACTTCCTAGCTAAGCATCGTCTCTTTCCTGCCCTGC AGCGTGTGGTCAGCCAGGCTGTAGACAAGCTCAGCCATGCCTGCCGTCACAATGGCTTCCCCCTCTTCCC AGTTACCTCAGAGACCATTCCAGATCTGCCTGGAAACTCCGATCTTCTGCAGCCCAGCTCTAAGGCTTCC ATACCCACCAACAGGGAGGCTAGGGGAGAGCCTTGTGATTCTCTCACCACAGCCTACAGCCCTAAGACAT CTCACAGAAAAAGCAAGGGCAGAAGGGGCTCCCCACCTAATGCAGTCCAGATGGCTACCAGATTCAGGCT CAAGGTGACACCTACACAAGTCTCTAATGTCCCTATCTCCTCATTCCACTCCATGCAGGAGGCCCCTAAC TCTGAACCCAAACTGCAGAAACAAGCTATGGCCTCAAACTGCAACCATATCTCTCAGCCCTGTCATGGCC TGCACCTCACCTTGCCTGCCCCTGGGATCACAGTTGAGGTGGCCTCCTGCCAGGGACGCCTCAGGGGCCC GGTCCAGCATCGTCTTGCCTCCCCCTGCTGTCTTCATTCCCATTTCCCTTTCCCTCTGTTCCCCCCATTC CTTTCTTTGGGAAAAAGCTTTTCCACTTCCCCTCCTACACTGCATCCAGAGGTGACCTCAAGAGCAGGGC TGGAGGTGTTAGAGCAACCTCTGAAGGGAAGGGGCTCCTTTACCCACCACTTCTAGTAGCCACTGTTACT GGCGGGTGCCCAAGGACAAGATCTCTTGCCCTGCATTGCAGAACTCTTCTGAGGTAGAGATAAGCTGCTT GGGAATCAGGTGTTCTTGTCCATCTATTTCCAGTTTCCAGATGTTGTCAAAGATTAATAAAGGCTATTTT TTACCCTGCTTTTTTTTTTCTTTTTTTCTTTTTCTTTTTTCTGAGACAGGGTATCACAAAGCTCAGGCTG GTCTCCAATTCATTATATAGTAGAGGAAGACCTTGGACTTGTGATCCTCTTGCCTCTACCTCCCAACCCC CTAAATGCTGGGATTATAGGCATGCATCACCATGCCTGCAGTTCAGGGTTTTTGTTGGTGGTGGTGTTTT TGTTTTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTTTGTGAACCTCAGAGGCCCTCCCACAT TTCTCTTATGGATATGAGGTTTTTAATGGTTTGATTTTCTTGTTTGTTTGCTTGCTTTTTTAGGACAGGG TTTCTCTGTGTAGCCCAGGATGTCCTGGAACTCACTCTGTAGAATAGACTGGCTTGGAACTCGGACATCT GCCTGCCTCTGCCTCCTGAGTGCTGGGATTAAAGGCATGCGCCTCAGCCGCTGCTGCTGCTGGCACTGCT GCCACCACCACCCAGCTGGACAGGAGTTTTTGCCAAGAGGGCAACTCTGTATCTTAAGGCCAAGTGTCCT CTAATCTAGCTTTAGGACTCTAGACTTTCTGCTCCATAAAGGGGACCTTGGTCAGGAAGTGTGTATGAAG ACATAGATGGCCTGACTTTGAAGGTCTCTAGACATCTCATCCTACCATCATCAGGGCTCCGATGTAATTC CTTCTGAACACAAGCTGTGTGCAAGCTTTCATGCACACAGCCTGCTCAGGCTGCTGGGCTTGCTCTAGAC CTGACAGGTGCAGCATATGGACTGAATGTTACAGTAGGCAGATGTGGCAGGCCTGTATCCTGGTGTGATG AGCTTGGTTTCTCTGACTGTAATGGGGGAAGATTGGCACAACGGCCAAGGTGGGTGTAGCAATATGATGT CACAGGGCAGCAGGGGGATGACAAGGGTTAAACGTCTCCTTTTCTCTCCTTGGCTGTTGCACAGAGCCCC AACACCAAGTTTTCGAAGAAGAAGCCTCTGCCCGCCATCTCATCTGTTTCCAGCTTGTCCTACATCTCTA ACCCCTGGTTTGAGGAACTCACTAACTTCTTGGTTGAGCAGGCAGTCTCCTTGCTTATCTGCAAGTACAA GTTTGAGGAAAGCCTCAATAAGCAACTTGGCTTCATCTCTTTTCCTATCACTGAGGTTCTCATGGACATC TTTCTGGGCTTCAAGAAGGTGAAGGGTACCCACATCCGCCTGTCCTCAGACATCAACTGGACCTGTCTTC TTCGTAAGTTGGAAGAAGCTGAGTTGTCACAGCAGGCCCTAAGACATGCCTCCAGGTCAGGAACCTCTCC ACGTAGTGTCTCCCGTATTGGCACCACCCAGCATAGCACAGAACCCTCCTCCATGCAACCGGAGCTTACT GGTGACACAGACCAGGACCAGTCTCCAGAGTCCTACCTCTCCCTCCACCCTGAGTTGCCAATCCACCAGC TGCTCAACTCTCAGGATCCTGGAACAGGCCAGGAACAGACACCTAACATGAACCTGTCAGAGGCCAAGCA CTCTATAATCTCCAATGCTAGTATCGGCTCTAGATCTTCCAAGACCAAGAAAGTGGAAAACACTGAAGAG GGTGAGAACGGTGAGGAGGAGGAAGAAGAGGAGGAGGAAGAAGAGGAGGAAGAGGAGGAGGAAGAGGAGG ACAGAGGAAGTAAGGAGGATGACACCAAAGATTTTTTTGAGGGTGAGCACACACCTCCTAGCCCGCCTTT GCATGAGTTGGAGAACTTTATGAATACAACCACTGTAATGAGCCCAAATAACTCCCCATGAGGCCTCTGT GAGCCACAAAGAGTACTCCTGTCCTATCCTAATAGCTGGCCACTCAGCCAGCCCTGTTCTGAGTGAAGGT CAGGATGACTGGGTGCTCAGGAGGAGCTCTGCTAGTATTATCAAGGGAGCCAAAGAGCCAAATGGAAATA AATACATGTTGGTGGACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6836034 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6836034 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13133 same embryo
	EMAGE:13132 same embryo
	EMAGE:13129 same embryo
	EMAGE:13131 same embryo
	EMAGE:13130 same embryo
	EurExpress:euxassay_017309 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source