EMAGE mouse gene expression spatial database

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EMAGE:13153

AI314180 expressed sequence AI314180 ( MGI:2140220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017280. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017280_01	euxassay_017280_02	euxassay_017280_03	euxassay_017280_04

euxassay_017280_05	euxassay_017280_06	euxassay_017280_07	euxassay_017280_08	euxassay_017280_09

euxassay_017280_10	euxassay_017280_11	euxassay_017280_12	euxassay_017280_13	euxassay_017280_14

euxassay_017280_15	euxassay_017280_16	euxassay_017280_17	euxassay_017280_18	euxassay_017280_19

euxassay_017280_20	euxassay_017280_21	euxassay_017280_22	euxassay_017280_23	euxassay_017280_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13153_wholemount_strong.wlz

13153_wholemount_moderate.wlz

13153_wholemount_weak.wlz

13153_wholemount_possible.wlz

13153_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13153_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9245
Entity Detected:	AI314180, expressed sequence AI314180 ( MGI:2140220)
Sequence:	sense strand is shown >T9245 CGAGTCCGCGTCTCTTCCTCCGTCCGGGCGCGTGTTCCTCCAGCTCCGACCAGGCGGACGTGACAGCCGC AGACCTTTAACGCCACAGCCCGGGGCGGGGAAGCTCTCCGCGGCAGCCAGGACAGCCCGGCTCCGCTCGG CCGCACGCAGGCTCCTCCGGAGCGGCCGCGCGTCTCCTCGGGCCCGGCCCGTCCTCCACCTCGCTGGGTC GCCGCAGCATCCCGGGCTATGGAGACCGGCTCCGACTCAGATCAGCTCGAACGGGTCTTTTTACGACTTG GCCATGCTGAAACAGATGAACAGTTACAAAATATTATATCTAAATTCCTTCCTCCTGTTCTGCTCAAACT GTCCAGCACCCAAGAAGGAGTACGGAAAAAAGTAATGGAACTGCTGGTCCATCTGAATAAACGTATAAAA AGCCGCCCCAAAATACAGCTTCCAGTAGAAACACTATTGGTTCAGTACCAGGACCCTGCTGCAGTTTCCT TTGTCACGAATTTTACTATAATTTATGTTAAGATGGGCTATCCTCGCCTGCCAGTGGAAAAACAATGTGA GCTGGCCCCTACGCTTCTTACTGCCATGGAAGGGAAACCTCAGCCACAGCAGGATAGCTTAATGCATCTT TTAATACCAACTCTTTTTCACATGAAGTACCCGGCTGAATCATCAAAGTCAGCTTCTCCTTTTAATCTTG CTGAGAAACCAAAGACTGTGCAGCTGCTCTTGGACTTTATGCTAGATGTCCTTCTGATGCCTTACGGATA TGTGTTAAATGAATCCCAGAGTCGCCAAAATTCATCATCATCATCACAGGGTTCTTCTTCAAACAGTGGG GGAGGTTCTGGAATCCCACAGCCTCCCCCAGGAATGAGCTTTTATGCAGCTAAACGAGTTATTGGTGACA ACCCGTGGACACCTGAACAGTTGGAACAGTGCAAATTGGGTATAGTGAAGTTCATAGAAGCTGAGCAGGT GCCTGAACTTGAAGCTGTTCTCCACCTGGTGATTGCTTCTAGTGACACACGCCATAGTGTCGCGACAGCC GCAGACCTGGAGTTGAAAAGCAAGCAGAGCTTAATTGACTGGAATAACCCTGCCATCATTAATAAGATGT ACAAGGTGTACCTTGGGGATATACCACTGAAAACAAAAGAGGGAGCAGTTCTAAAGCCGGAGCTGAAAAG AGACCCAGTCAGTACTAGAGTGAAGTTAAAGATTGTTCCTCATCTCCTGCGCTCTAGACAAGCTGCTGAA ACGTTCCCAGCTAACATTCAGGTGGTATATGATGGACTTTTTGGTACAAATACAAACTCAAAGTTAAGAA CACTGTCCCTGCAATTTGTACATCACATTTGTTTAACTTGCCCAGAAATCAAGATTAAACCACTAGGTCC TATGCTTTTGAATGGTCTCACTAAGCTAATAAATGAATATAAAGAGGACCCTAAACTACTGTCAATGGCA TATTCAGCTGTTGGGAAACTCTCCAGTCGAATGCCCCATTTATTCACTAAGGATATAGCTCTTGTGCAGC AGCTCTTTGAAGCCCTCTGTAAGGAAGAACCGGAGACTCGGCTTGCTATTCAGGAAGCGTTATCTATGAT GGTTGGAGCTTACAGTACCTTAGAAGGAGCACAGAGAACTTTAATGGAGGCACTTGTAGCTTCATACTTA ATAAAGCCTGAAGTTCAAGTTCGGCAAGTTGCAGTGAAATTTGCCAGCACGGTGTTTCCCTCAGATCATA TACCTTCCAGATACTTGCTCCTACTAGCTGCAGGAGACCCACGAGAAGAAGTCCATGGTGAAGCACAGCG CGTATTACGATGTCTTCCTGGTAGAAATAAAAAGGAAAGTGCTTCTAAGCAGATGCCATCTTTTCCAGAA ATGGTATATTACATTCAAGAAAAGGCTTCCCATCGAATGAAAACTCCAGTCAAATATATGACCGGAACCA CAGTCCTTCCATTTAATCCAGCAGCATTTGGAGAGATAGTTCTGTACTTGCGCATGTGCCTGGCTCATAG TGCAGGGGTGGTACCCACTTCCCAGAGTTTGGCTGATATGCAAGATCATGCTCCGGCTATTGGACGGTAC ATACGGGCTTTGATGTCAAGCAGCCAGGCGACAGCTTCATCTTCTAATAAGAGTGGAGAAACCAACCCTG TTCAGATCTACACTGGCCTGCTTCAACAGCTGCTAGCGGGGGTTGGAGGTTTGCCAGTCATGTACTGTCT ATTGGAAGCTGTGTCAGTGTATCCAGAAAAATTGGCTACCAAATTTGTAGACAAAACAGAATGGATCAAG AGTCTGATGAGTAGCAGTAAAGAAGAAATGCGTGAATTGGCGGCTTTGTTTTATTCTGTGGTTGTGTCTA CAGTGTCCGGAATTGAGTTAAAGTCAATGATAGAACAGCTGATAAAGGCCACAAAAGACAATCATAGCCC TGAGGTTCAACATGGATCGTTGCTTGCCTTGGGATTCACTGTTGGAAGATACTTGGCTAAGAAGAGAGTG AGGATGGCCGAGCAGCATGACCTAGAGACAGATGCTGACCTCTTGCCTGAGCAAGAGGAGATCATTCGGA GTGCTACAGAAACAATAGGTTCATTTTTAGACAGTACATCACCTCTGTTAGCAATTGCTGCCTGTACAGC CTTGGGTGAAATCGGCAGAAATGGTCCTCTTCCGATCCCCAGTGAAGGGTCTGGATTTACTAAACTGCAC CTCGTAGAAAGCTTACTCAATAGAATTCCGTCTAGTAAAGAAACAAACAAGATGAAAGAACGAGCAATCC AAACACTGGGCTATTTTCCTGTTGGAGATGGGGTTTTTCCCCACCAGAAACTCCTTTTGCAAGGTCTGAT GGATTCTGTGGAGGCCAAGCAGATTGAGCTTCAGTTCACTATTGGTGAAGCCATTACCAGTGCTGCAATA GGAACTAACTCTGTGGCTGCCCGAGATGCCTGGCTGGTGACTGAAGAAGAATATATACCTCCTGCTGGTG CCAAAGTGAATGATGTTGTCCCATGGGTTTTAGATGTTATTTTAAATAAACATATCATCAGCCCCAACCC ACATGTCAGGCAAGCAGCCTGCATCTGGCTTCTTTCTCTTGTGAGAAAACTAAGTACCCACAGAGAAGTG AAGTCCCATCTTAAAGAAATTCAGAGCGCATTTGTTTCAGTTCTGTCAGAAAATGATGAACTCAGCCAAG ATGTTGCCTCAAAGGGGCTTGGGTTGGTTTATGAGCTGGGCAATGAACAAGATCAACAAGAATTGGTGTC TACACTTGTAGAAACACTTATGACTGGCAAAAGAGTCAAACATGAAGTTTCTGGAGAGACAGTGGTATTT CAAGGGGGTGGCCTTGGCAAAACACCTGATGGTCAGGGCCTTTCTACTTACAAGGAGCTTTGCTCTCTGG CAAGTGATCTCAGTCAGCCAGATCTGGTATATAAATTTATGAATCTGGCAAATCATCATGCAATGTGGAA CTCCAGGAAGGGTGCTGCTTTTGGTTTTAATGTAATTGCTACCAGAGCTGGAGAACAACTGGCTCCTTTT CTGCCTCAACTAGTTCCTCGCCTTTATCGATACCAGTTTGATCCCAACCTTGGAATTCGACAAGCTATGA CAAGCATTTGGAATGCATTGGTCACTGACAAGTCAATGGTGGATAAATATTTGAAGGAAATTCTTCAAGA TTTGATTAAAAACCTTACCAGCAACATGTGGAGAGTTCGAGAATCCAGCTGTTTAGCTCTGAATGATTTA TTGAGAGGAAGACCTTTAGATGACGTCATTGACAAACTTCCAGAAATGTGGGAAACACTTTTTAGAGTAC AAGATGATATTAAGGAGTCTGTACGGAAGGCAGCAGAACTGGCTCTGAAAACTCTGAGCAAGGTTTGTGT GAAAATGTGTGACCCTGCCAAAGGAGCAGCTGGCCAGAGAACCATTGCTGTCCTCCTGCCCTGCCTTCTG GACAAAGGAATGATGAGTCCTGTGACGGAAGTCCGAGCCCTCAGCATTAACACTCTTGTGAAGATCAGCA AAAGTGCAGGAGCCATGTTGAAACCACACGCACCCAAACTCATACCAGCACTCTTAGAGTCCTTAAGTGT GTTGGAGCCTCAAGTTCTCAATTATTTGAGTCTCCGGGCTACAGAACAAGAAAAGGATGTAATGGACAGT GCTCGACTCAGTGCTGCCAAATCCTCTCCCATGATGGAGACAATCAACATGTGCCTGCAATATCTTGATG TTTCGGTGCTAGGTGAACTAGTTCCTAGGCTATGTGAGCTGATCAGAAGTGGTGTAGGTCTTGGGACTAA GGGGGGCTGTGCCAGTGTCATCGTGTCTTTAACTACTCAGTGTCCCCAGGACCTGACACCTTACTCAGGT AAACTTATGAGTGCTTTGCTTAGTGGTCTGACAGATCGGAACAGTGTAATTCAGAAATCCTGTGCATTTG CCATGGGCCATTTAGTTCGGACTTCACGGGATAGCAGCACTGAGAAACTGCTACAGAAGCTCAATGGCTG GTACATGGAGAAAGATGAACCTGTCTATAAGACGTCTTGTGCTTTGACAATTCATGCAATTGGAAGATAC AGCCCAGATGTATTGAAGAACCATGCTAAAGAAGTTCTGCCCTTAGCATTTCTAGGCATGCATGAAATTG CTGATGAGGAGAAATCTGAAAAAGAAGAGTGTAATATGTGGACTGAAGTGTGGCAGGAAAATGTACCTGG CTCCTTTGGAGGTATTCGATTGTACCTGCAGGAATTGATTACTATTACCCAGAAGGCTTTACAATCACAA TCTTGGAAAATGAAAGCCCAGGGTGCAATTGCTATGGCATCAATTTCAAAACAAACAAGCTCTCTTGTAC CTCCTTACCTGGGGATGATCCTGAGTGCATTGATGCAAGGCCTGGCTGGAAGAACATGGGCAGGAAAGGA AGAACTATTGAAAGCCATTGCCTGTGTAGTGACGGCTTGCAGTACAGAGTTGGAAAAGTCTGTGCCCAAT CAACCCACCACAAATGAAATTCTTCAGGCTGTCTTGAAGGAGTGTTGCAAAGAGAATCTCAAATATAAGA TTGTTGCAATCAGCTGTGCAGCTGATGTTTTGAAAGCCACCAAAGAAGACAGATTCCAGGAGTTTTCTGA CATTGTCATACCCCTCATCAAGAAGAACTCACTTGAAAGTATGGGAGTCCGTACCACCAAAGCTGAAGAT GAAAATGAGAAGGAAAGAGAGCTGCAGCTGGAGTCTCTACTGGGCGCCTTTGAGAGCCTGGGCAAAGCAT GGCCCAGGAACCCAGACACCCAACGTTGTTATCGGCAGGAGCTGTGCAAACTGATGTGTGAGCGGCTAAG ACTCAGCACGTGGAAAGTGCAGCTAGGTGTCCTCCAGTCAATGAACGCCTTTTTCCAGGGGTTAATGCTT CTGGAAGAGGAACACGCAGATCCTGAAGCTCTGGCTGAGATTCTTCTCGAAACATGCAAATCAATTACTT ACTCTTTAGAAAATAAGACCTACTCATCTGTGAGAACAGAAGCTTTATCTGTGGTAGAATTACTGCTTAA AAAACTTGAAGAAGCTAAACAATGGGAAAGTTTGACAGCAGAATGCAGAGGACTTCTGATTGAGTCTTTA GCTACTATGGAGACAGACAACAGACCAGAGCTGCAGGAGAAAGCCTCAGTATTGAAGAAAACACTTGAAA GTCTGGAATGAATGAGAAGGGGAAGAAACAAACAAGTGCCATGTTCTTTTGGGATTGGAAATGGTGGTAT TTAAAAAAAAAAAAACCAAGTGGGGGAAAGTAAAGGTTACTCTGTAGCATGCATCATTCCGTGGCTGAAA TAAAAAGAAAAAAAGCCTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834912 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834912 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13152 same embryo
	EMAGE:13154 same embryo
	EMAGE:13151 same embryo
	EurExpress:euxassay_017280 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source