EMAGE mouse gene expression spatial database

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EMAGE:13177

Ece1 endothelin converting enzyme 1 ( MGI:1101357)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017226_01	euxassay_017226_02	euxassay_017226_03	euxassay_017226_04	euxassay_017226_05

euxassay_017226_06	euxassay_017226_07	euxassay_017226_08	euxassay_017226_09	euxassay_017226_10

euxassay_017226_11	euxassay_017226_12	euxassay_017226_13	euxassay_017226_14	euxassay_017226_15

euxassay_017226_16	euxassay_017226_17	euxassay_017226_18	euxassay_017226_19	euxassay_017226_20

euxassay_017226_21	euxassay_017226_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13177_wholemount_strong.wlz

13177_wholemount_moderate.wlz

13177_wholemount_weak.wlz

13177_wholemount_possible.wlz

13177_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13177_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9376
Entity Detected:	Ece1, endothelin converting enzyme 1 ( MGI:1101357)
Sequence:	sense strand is shown >T9376 GTAGAGCTCAGCCCAGCGCACTTGGAGCTGGAGCCTTAGCGGGAGGTGCATCCTAAGCCCGGCCGTTCGG AGCCCGGGAGCGACGGCGCAGCCTGCCCACCAGGGTAACACAAATACTAATTACCACTACTTCCACAAGC TGTTTCCTGGTTGAACCTCATTACCGAGCAGATGTCAACCTACAAGCGGGCCACGCTGGACGAAGAGGAT CTGGTGGACTCACTCTCCGAGGGCGATGTGTACCCCAATGGCCTACAGGTGAACTTCCGCAGCTCCCGGA GCGGCCAGAGGTGCTGGGCAGCTCGGACCTCGGTGGAGAAGCGGCTGGTGGTGCTGGTGACGCTTCTGGC GGCAGGGTTGGTGGCCTGCCTGGCAGCCCTGGGCATCCAGTACCAGACAAGAACGCCTCCGGTATGTCTG ACGGAGGCCTGTGTCTCAGTGACCAGCTCTATCCTAAACTCCATGGACCCCACAGTAGACCCGTGCCAGG ACTTCTTCAGCTACGCCTGCGGGGGCTGGATCAAGGCCAACCCCGTGCCCGATGGCCACTCACGCTGGGG AACCTTCAGCAACCTCTGGGAACACAACCAAGCTGTCATCAAGCATCTACTGGAAAATGCCACGGCCAGT GTGAGCGAGGCAGAGAGAAAAGCCCAAGTGTACTACCGTGCGTGCATGAACGAAACTAGGATTGAGGAGC TTCGGGCCAAGCCCCTGATGGAGCTGATTGAGAAGCTGGGAGGCTGGAATATCACAGGACCCTGGGCCAA GGACAACTTCCAGGACACACTGCAGGTGGTCACAGCTCACTACCGCACCTCACCCTTCTTCTCTGTTTAT GTCAGCGCGGACTCCAAGAATTCCAACAGCAATGTGATCCAGGTGGACCAGTCCGGCCTTGGCTTGCCCT CCAGAGACTATTACCTGAACAAGACAGAGAATGAAAAGGTCCTGACTGGATACCTGAACTACATGGTCCA GCTGGGGAAGCTCCTGGGCGGTGGGGACGAGGATGCCATCCGGCCACAGATGCAGCAGATCCTGGATTTT GAGACGGCGCTGGCCAACATCACCATCCCCCAGGAGAAGCGCAGGGATGAAGAACTCATCTACCACAAAG TCACGGCTGCCGAGCTGCAGACCTTGGCACCTGCCATCAACTGGTTACCCTTTCTGAACACCATTTTTTA CCCAGTGGAGATCAATGAGTCTGAGCCAATCGTGGTCTACGACAAGGAATACTTGAGACAAGTCTCCACC CTCATCAACAACACCGACAAATGTCTGCTCAACAACTACATGATGTGGAACCTGGTACGCAAAACAAGCT CCTTCCTGGACCAGCGCTTTCAGGACGCCGACGAGAAGTTCATGGAGGTTATGTATGGGACGAAGAAGAC CTGTATTCCCCGCTGGAAGTTTTGTGTGAGTGACACGGAAAACAACCTGGGCTTTGCCCTGGGACCCATG TTTGTGAAAGCAACCTTTGCAGAAGACAGTAAGAATATAGCCAGTGAGATCATCATGGAGATCAAGAAGG CGTTTGAGGAGAGTCTGAGCACTCTGAAATGGATGGATGAAGAGACTCGGAGATCAGCCAAGGAGAAGGC GGATGCTATCTACAACATGATAGGCTACCCCAACTTCATCATGGACCCCAAGGAGCTGGACAAAGTCTTC AATGACTACACAGCAGTTCCTGATCTCTACTTTGAGAACGCCATGCGCTTTTTCAACTTCTCATGGAGGG TCACAGCTGACCAGCTCAGAAAAGCTCCCAACAGAGATCAGTGGAGTATGACGCCGCCCATGGTGAACGC TTACTACTCGCCCACCAAGAACGAGATCGTGTTTCCAGCTGGAATACTGCAGGCGCCATTTTATACCCGC TCTTCACCCAACGCCTTGAACTTCGGTGGCATTGGTGTCGTAGTGGGCCACGAGCTGACTCACGCTTTCG ATGATCAAGGTCGGGAGTACGACAAGGATGGGAACCTTCGACCCTGGTGGAAGAACTCGTCCGTGGAGGC GTTCAAGCAGCAGACGGAGTGCATGGTGCAGCAATACAGCAACTACAGCGTGAACGGGGAGCCCGTGAAC GGTCGACACACCCTCGGGGAGAACATTGCCGACAACGGGGGACTCAAGGCGGCCTACCGGGCGTACCAGA ACTGGGTAAAGAAGAACGGAGCTGAGCAGACGCTGCCCACCCTGGGTCTCACCAGCAACCAGCTCTTCTT CCTAGGATTTGCACAGGTCTGGTGCTCTGTCCGCACACCTGAGAGCTCCCACGAAGGCCTCATCACGGAT CCGCACAGCCCCTCTCGCTTCCGGGTCATCGGCTCGCTCTCCAACTCCAAGGAGTTCTCAGAACACTTTC GCTGCCCGCCTGGCTCTCCCATGAACCCTCATCACAAGTGCGAAGTCTGGTAAGGGCTAAAGCGCAGAGA GCACGGACAGAAAAAGGGAAGGAGCTGCAGCTAGCTCCCAGCGGAGCCATGTTGCCACCCTCCTCCCAGC CCCTCGGCCTAGGGCCCCTTCCCCACTCTGGAGAGTATGCAGCCATCCTAAGCCTAAGATGGATGGAGCC TCACCACTCGAAGCCAAGATTTGACCCCCTGTGAAGCTCCGGCATCCTGGACACCGGTGCGTGATGCTAG GCGGGCCTTTGGATGTGTCAAGCTGGTGGCTTGCCAGCCCTGGGCCTCACACTGACAATGGCAGTGGGAC ATGGCCCCCTGCCCACATCCAATGCCAGATACACCACAATACCACTGTGTCAAATGCTTTAAAGATATAT TTTTGGGGAGACTATTTTTTAAGCATTATGGAATACACTGGAAATCTTCAGGGAAAATACATTTAAAACA CTTTTTTTTAAAAAAAGAAAAGAAAAGATTAGTATATTTATTATGTTCTCTTTTTTCTAAACAACCTGTG GACAAAGGAAACCCCACTAATTTACCCCCTGGGAACCCCAGGCTGTGGAGAAGGCTGCCAGTTTGAGCGA GCACTACTTTAGCCCATTGCTGGTGTAATTACTTCTATAGTCACCGGTGCAGTCAGGAGATGTAGGGGCC CAGGCAGAGGGGGTGAGCAGCTGAAGGGCCTGGTTATGGGCGTGACCTTCTCAGCTTACCTGTCCTGTCG CTGGAGTCCAACCATGGGAAGCCCAACAAGGATGGGTTGATACCCAAGTGATAGTTGAGGCAGCACAAAG GCAGAGTAATGACCTTCATATCACGTGGCACCCGAACACTCTAGACTAGAACATAGGAGCCTGTTGGAGC TGAGAACCCACACCCCACCAGATGCACCCTGTTGTCCAAAGATGTCTTTTTTTTTTTTTTTTTTGGTCCC ACCTCTTCTGGCCCTGAGACCCGTTGCCTCTCTGTAGCAATTCTGACATCCTGAAGTGGTCAGCTTCACT GATCCTCTGTGCAGAGGGAAAGGGGAAAAAAAACTCAGCCCAGTTCTCCCTCCAAGCTCTGTAGCCTGTA GTTGCCCTGGCCTGGCTCCTGGGACCCCCTTCTCTAGTGCCTTACCCCAGGCTGCAGACCCTGAGCCCCA CTAGGAGGCAGCATTTGGCTTGCTTTCCCAGTGGAGCCCCCAAGTGTCCTGACTAGAAGCCAACACCATA GCCCCATTCCCAGAAGCCCCAGGGTACCACCCCAACCTGCCTACAGGACAGCCATTCCAGATAGACGTCC AGTGTCAGGAGCTGCTGACCTCTCAGGCTTTCAGCCACCCGTGGCACGCACATTGAATCAATTCAGTCCT TGTAGATAGAGGGATTCCCCTCACCCCCTCCCCCTCTCTGTTTTTTTGGAGGTGGATGCTGTAGCCAGAG CAGGCCAAGACTGGAATGTTCCCAGCCCCATTGGGCTCCAGGGAATGGCAGAAAGTCATCCACCCACAGC ATCACGTAGTAGATATGTGGGCTCCCACTAGGTGGCGCTCACCGCAGTTCTCATGACTCCTGCAGGGAGC AAGCTCTTGTGATATGTGTGGGAGAAGCCTAGTAGTGCCCATTACAGCCCCTGGCAGGCCATGCTCCCAC ACCACACAGCACGGCGTGCGCTCACACACACACACACACACACACACACACACACACACACACACACACA CACACACACACACACCTGGAAGCCGGAGTCCTCCTTGACCAAGACGCAGAGACAATATGGTCTCGGCCCC GCTTGCTAGGATGGGAACTACAAGCAGGCACCTGGCAAGGTTCTTGGTCCCTGTAAACACATTCGAGGTC TCGGCTCTTCGGGGGAAAGGTAACATAAGAAGGCAGGAAGGTGCTGGAGCCGTGCCCTGCCACACAAAGG GGGACCTTCTGGGTCTGGATCATCTGCCCTTTCTATCCCGTCGCCTTGCCTCTCCATAGAGTGGCAGTCC TGGATACTGGTATCTAGAAGCGGGGTCCTGAACTGGGTTCAGCCATATGGACATTGCTCAGGGTGTGCAG GGAGCCAAGGCAGGAAGACGCAGACTGGTTAGGGATGGATGGGCACAAAAGCAGAACCCCAAGCCCCGCC CCTCCAGAGATTTGAGAAGGGCGGTAGCGGGAAGGGCATCTGCCCTCAGAAAGACCCCTTCTCTCAAGCC CGGAGTTTCCCTGCAGGCACAAGGACATGGGGTTTGGAGCTGGGGACTCTTATTTTTTTGTATTATTGTG TTTTGTGCTACTGTAGTTTTGGTGTGGCACTATTATAATTAAAATAAAGTACTTATACCAAGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6854311 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6854311 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13180 same embryo
	EMAGE:13178 same embryo
	EMAGE:13181 same embryo
	EMAGE:13179 same embryo
	EurExpress:euxassay_017226 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source