EMAGE mouse gene expression spatial database

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EMAGE:13179

Edil3 EGF-like repeats and discoidin I-like domains 3 ( MGI:1329025)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017227. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017227_01	euxassay_017227_02	euxassay_017227_03	euxassay_017227_04

euxassay_017227_05	euxassay_017227_06	euxassay_017227_07	euxassay_017227_08	euxassay_017227_09

euxassay_017227_10	euxassay_017227_11	euxassay_017227_12	euxassay_017227_13	euxassay_017227_14

euxassay_017227_15	euxassay_017227_16	euxassay_017227_17	euxassay_017227_18	euxassay_017227_19

euxassay_017227_20	euxassay_017227_21	euxassay_017227_22	euxassay_017227_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13179_wholemount_strong.wlz

13179_wholemount_moderate.wlz

13179_wholemount_weak.wlz

13179_wholemount_possible.wlz

13179_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13179_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9381
Entity Detected:	Edil3, EGF-like repeats and discoidin I-like domains 3 ( MGI:1329025)
Sequence:	sense strand is shown >T9381 GTCAGAAGCCCCCGCAGCCGCCGCGGCAGCAGCGTGAGCCGTAGTCACTGCTGGCCGCTTCGCCTGCGGT GCGCGCACGGAAATCGGGGAGCCAGGAACCCAAGGAGCCCGCGCTCCGCCCGCTGTGCCTCTGCTAGGAC CCACTCGCAGCCCCAGCCTCTCTCAAGCGCACCCACCTCCGCGCACCCCAGCTCAGGCGAAGCTGGAGTG AGGGGTGAATCACCCTTTCTCTAGGGCCACCACTCTTTTATCGCCCTTCCCAAGAATTTGAGGAAGCGCT GCGGGAGGAAAGACGTCCTCTTGAATCTCTGACAGGGCGGGGTTTACTGCTGTCCTGCAGGGCCGCCTCG CCTACTGTGCCCTCCGCTACGACCCCGGACCAGCCCAGGTCACGTCCGTGAGAAGGGATCATGAAGCACT TGGTAGCAGCCTGGCTTTTGGTTGGACTCAGCCTCGGGGTGCCCCAGTTCGGCAAAGGTGACATTTGCAA CCCGAACCCCTGTGAAAATGGTGGCATCTGTCTGTCAGGACTGGCTGATGATTCCTTTTCCTGTGAGTGT CCAGAAGGCTTCGCAGGTCCGAACTGCTCTAGTGTTGTGGAGGTTGCATCAGATGAAGAAAAGCCTACTT CAGCAGGTCCCTGCATCCCTAACCCATGCCATAACGGAGGAACCTGTGAGATAAGCGAAGCCTATCGAGG AGACACATTCATAGGCTATGTTTGTAAATGTCCTCGGGGATTTAATGGGATTCACTGTCAGCACAATATA AATGAATGTGAAGCTGAGCCTTGCAGAAATGGCGGAATATGTACCGACCTTGTTGCTAACTACTCTTGTG AATGCCCAGGAGAATTTATGGGACGAAATTGTCAATATAAATGCTCTGGGCCATTGGGAATCGAAGGTGG GATCATATCTAATCAGCAAATCACAGCTTCATCTACTCACCGAGCTCTTTTTGGACTCCAGAAGTGGTAT CCCTACTATGCTCGACTTAATAAGAAGGGCCTTATAAATGCCTGGACAGCTGCTGAAAATGACAGATGGC CATGGATTCAGATAAATTTGCAAAGAAAAATGAGAGTCACTGGTGTTATTACCCAAGGAGCAAAAAGGAT TGGAAGCCCAGAGTACATAAAATCCTACAAAATTGCCTACAGCAATGACGGGAAGACCTGGGCAATGTAC AAAGTAAAAGGCACCAATGAAGAGATGGTCTTTCGTGGAAATGTTGATAACAACACACCATATGCTAATT CTTTCACACCCCCAATCAAAGCTCAGTATGTAAGACTCTACCCCCAAATTTGTCGAAGGCATTGTACTTT AAGAATGGAACTTCTTGGCTGTGAGCTCTCAGGCTGTTCAGAACCTTTGGGGATGAAATCAGGGCATATA CAAGACTACCAGATCACTGCCTCCAGCGTCTTCAGAACACTCAACATGGACATGTTTACTTGGGAACCAA GGAAAGCCAGGCTGGACAAGCAAGGCAAAGTAAATGCCTGGACTTCCGGCCATAACGACCAGTCACAATG GTTACAGGTTGATCTTCTTGTCCCTACTAAGGTGACAGGCATCATTACACAAGGAGCTAAAGATTTTGGT CACGTGCAGTTTGTTGGGTCATACAAACTAGCTTACAGCAATGATGGAGAACACTGGATGGTGCACCAGG ATGAAAAACAGAGAAAAGACAAGGTTTTTCAAGGCAATTTTGACAATGACACTCACAGGAAAAATGTCAT CGACCCTCCCATCTATGCACGATTCATAAGAATCCTTCCTTGGTCCTGGTATGGAAGGATCACTCTGCGG TCAGAGCTGCTGGGCTGCGCAGAGGAGGAATGAAGTGCGGGGCCGCACATCCCACAATGCTTTTCTTTAT TTTCCTATAAGTATCTCCACGAAATGAACTGTGTGAAGCTGATGGAAACTGCATTTGTTTTTTTCAAAGT GTTCAAATTATGGTAGGCTACTGACTGTCTTTTTAGGAGTTCTAAGCTTGCCTTTTTAATAATTTAATTT GGTTTCCTTTGCTCAACTCTCTTATGTAATATCACACTGTCTGTGAGTTACTCTTCTTGTTCTCTGAATT CTATAGATTGGTTGAAGTATAGTAGGCAAAGCAAGTCTTCTTTTTACAGCAAAACTTAAAAACAATCTCA CAGTCATCAGATGGAAGGGTTAAGGGAGAACTTTGGTCACTATTCAATTAACTCTTATAAAATGTTACAA TGTTAGCAATACAGTTTTTTGTTATGAATTTGATCATCTTGATTGTTTCCCTGTGTCTAATGCTGTTAGT ATGTATTACAGAGACTTGGTGAAAATTATTTGATATGCAGGACCATTAATATGACAATTCTAGTTTTATT TTTTTCACTTACAATAAAATACTAAGTTCTTTATGTTTAATTTTATATTATTTATATGCTACATAAACAG GCCAATATTCTTTGTCATTGCATTTTCTCCCTTTATTTACCTTAAAGTCCTGTTTTTATTTATAGACACA GAACATATATTAATATACGTTGAACATGATTGGATATCAGTAAATGCTTCTATATTAAACATTAATACAT TGAATCTGAATTTTCATTTTTGAAGAAAGCTATATGCATTTCATTTCCAAAGGCTATCTGAGATTCCCTT GTTACAGTTTAGTGAAAAAGAACAGAAAAACACTATGGCCCTTGAATTTAAATCTTCATTTCTTTGAGAT AACAGAATCACTATCTGTGTGTTTAATACACTAGCAAATGTATGTGAGCCTACTGCTGCACAGCTGGGCT AAGTGCACTCAACAATTCTTTGCAAATTTCCACATTTCCTGTGAAATGAAAATTCCAATGCTGTTTAAAA CCAGTGGTAGTTTCTGAAGAAAGCACATTGTAAAATTATTTGTGAGAAGCGACGTATTTACAAATGACAA AGTCATAAAACATCTTCAGGATGAAAATTGATTTTTATTTCTTTTGCTTCAAAGGTCTTAGAAAAACTAA GCAATTATCATAACAGTTTGTTATTGATAATGGAGGCATCATTGACATATGTCTCAAATTAAAGCTCACA CTGCCTCCATAAATGTCCTACATACCTAATTTATAAGCTTAACACATATTTATTTTATAAGGCTTTGACA CAGAATTATTGGAGTTTTAAATTGAAGATCCAGGAAAGGAAAGGGTGGATGTGGATGAAATGCTAAAGAA CTACACAACACTGCTATATTTTATTTTATTTTTGTCTTTAATATGTGTGCTGTATAAGCAAAGCCACTAC TGTTATGTCCTGTCTGTTCACGTGTTCACAGCATCTCTCAATGATGTAAATATGAAGCAGTCTTCAATCA CAGTGAAGAATTGAAAGTGAAAGTCAATCACATTACTGTTTTAAATGACAGCAAATTCATTGTTCTATGG TAACTGTCAGGCCAAATGTTTCTCCTTGCCCACCTGCTTACTGCAACAGATTTGTTAAAAATCTGTTGAT TTTCAGCATATAAAATGCTGAATAATTTTTAACATTTTCCCAAAAGAAAACTGATATGCCCTCAATATTA TTGTCCTAGAAAATGTTTTAAATTATTAATATAGAATATTTAAAATAGTATTTTTAATTAAAATAGTTAT ACATAGAATCACTTATGCCCTAAAGGTACATTTTCTGTCTTGCCATGGTCCTTGTTTCTATCAGTGATAA CCATACTACTGAATTTGTAGCTGTTTTTCCTTGCAGATTCTTTATCCTTAATATAAAATTCCAGAAATTA TGAATAATACGCTGTGCTGAAATACTTTTCAAAACTGTTCTTGATTTCATGTGGCACTGCTGGTACATGT TTAAAATTTGTTCTCCAAAGGAGTTGTTAGTAATGGTATCTGCTACTATTTACAAGGCTGGTGAGAGGCT TTCTCTTGCTTGTCCTCTTCCTCTCAGCTTCATGCGCTACCTACAGCATAGCTTACGCAATGGATATGCG TTTGTCCACAAGGGTGCTTGCACAAAAATTAATGACATGTATTAGGGAGTAGCTTGTATCAGTGGCTTCA AGTCGAAACATGGAATTTTCATTTAGTTTTATTTGCAAGTAAAATTTTACAAACACACAACCTGGAATTC CCTACTCTTCACTATACTACATAATTATTATGAGATAATTTCATTGTATAGAAGATTATGAATTAATTAG CTTTAAAAATATCAATTTCATGTATTTTATTCTAATCATTTGTCACAGACACTAATTCCAACTTTAGGGT TGACTTTCTCTCTGTGAATGTCTTCAGAAAGATAAGAATGGATCTTGAAGACCAGGCATTAATGACTATA TCTTTGCTAGACATCAAATTATGAAATATAACATTGAATGTTGTAGTCTGGTGTGTCTGTAAGGACAGAA ATAACTAAGATACACATAACTTTTGCAAACTTTCAAGCTGTGTAATATTCCAATGTTGTTGTTTTTTTTT TTGTAGATATACTTAAATCATGTAGGATGTTGTAAACTCAGTATGATCCTGTCTAGTCTTCAAACTTAAA ATAAACTTTTGAAAAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6854497 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6854497 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13180 same embryo
	EMAGE:13178 same embryo
	EMAGE:13181 same embryo
	EMAGE:13177 same embryo
	EurExpress:euxassay_017227 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source