EMAGE mouse gene expression spatial database

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EMAGE:13180

Fam123c family with sequence similarity 123, member C ( MGI:3026939)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017223. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017223_01	euxassay_017223_02	euxassay_017223_03	euxassay_017223_04

euxassay_017223_05	euxassay_017223_06	euxassay_017223_07	euxassay_017223_08	euxassay_017223_09

euxassay_017223_10	euxassay_017223_11	euxassay_017223_12	euxassay_017223_13	euxassay_017223_14

euxassay_017223_15	euxassay_017223_16	euxassay_017223_17	euxassay_017223_18	euxassay_017223_19

euxassay_017223_20	euxassay_017223_21	euxassay_017223_22	euxassay_017223_23	euxassay_017223_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13180_wholemount_strong.wlz

13180_wholemount_moderate.wlz

13180_wholemount_weak.wlz

13180_wholemount_possible.wlz

13180_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13180_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9371
Entity Detected:	Fam123c, family with sequence similarity 123, member C ( MGI:3026939)
Sequence:	sense strand is shown >T9371 CGGGCACGGCCGCGCCCTGCGACACTCCCCAGCCCCGGCGTGGCAAGAAGACTGTCAACATGGAGCTGAG GAGAGGAAAGACCTTCATCAAATCTAGCGTGCAGATTTCTCACGAGAAACTCATAGACTCACCAGCCAAG GAGGACCCAGACAAGTGGCCACTGTCGCTGGGAGAGCAGCAGCGAGCCTATGGCGAGAAGAGCTCCCAAA CAAGTCCCTGTTCCCAAGGATACGGCCGGTGCCCCAACAAAGAGGTCCTGTCTGATCCTGAAGGGGGTCC CGTGCCCCTCTGTGGAACCACCTTCAAGTTGGTGCGCAAGAGTAAGACTCATGACAGTGTTCCAGGGGCT GTGAAGGCGGCGGCCCCCACAGGGCAGATGGTAGGTAGCACAAGCTTCTCAGAGACCCCAGGAGGTCAGC GCATGATCGACTACCGCCACTTTGTGCCCCAGATGCCCTTCGTGCCAGCTGTGGCTAAGAGCATCCCAAG AAAAAGGATTTCTCTGAAAAGGTCCAAGAAGTGCTTTCGAAACCTATTTCACATGCGCAGAAGCAAGACT GAGAACTTGGCCTCGCTCTCAGCCAAGGGGAAGAACCTCTCACCCTCCGGGGTCCCAGCGCAGCAAGGCA CGGCTTTCCTCTCCATGGGTGAGGGGCTGGGGCTGGACAGCCTATGCCAGGACCTGTCTGACAGTGAGTT TCTACATGACTCACCCTTTGACCTCTGCAGCGCCCTGTGTGAGGATGTGGCCTCACTGAAAAGCTTCGAT TCACTTACAGGTTGTGGGGAGATCTTTGCAGATGGGAGCTCGGTGCCATCTGTGGAGCTGAAGGACGGCC CAGAGAGCCCAGCCCATTCACCTCAGGCTCTGGACTGCAAGACTCCCTGTGGCCCCGCCCAGGGTGGTAT GGAACAGCTGATGTCACCTGCCCAGAATGAAGCATCAGACTTCAACAAGTTCTGGGACAGTGTGAATCGC TCTGTGCAGCAGCAGCAGCGTGCTCTGATGGGCCCATGGTTGACGAGTCCCGAAGGGACAGAAACAGACC AGACCAGGCTAGACACATCTGGATTAGCTGAACTACCCCTGTTCCCTTGCAGGGGTCCCCCCAGCGGTTC CAAAGCCAGCTCCATAGACACAGGTACCCCCAAAAGTGAACAGCCAGAATCTGTGTCCACAAGCGATGAA GGTTACTATGACTCCTTCTCTCCAGGCCTTGAGGAGGAGAAGAAGGAAGCTGCGAGCCCAGGCACGCCTG CAGCCACTTTCCCCAGGGACAGCTACAGCGGGGACGCCCTCTACGAACTATTCTATGACCCCAGCGAGGC CCCTGTTGGCCCAATCCTGGATGATGACTGTGTGTCTGAGAGTCTCTCGGGGCCTGCCCTAGGGACCCCG TTGTCTATGTGCAGCTTCCGGGTGGGAGCCGAGGAGAACCTGGCCCCAGCGCCAGGCCCTGACTTGCTCA GCCAGGGTTTCCTACAGAGTACCTGGAAGGGCAAGGAATGTTTGCTGAAGCTCTGTGACACAGAACTTGC CATTACCATGGGCATTGTCAACTGGCTGCGCAGAACCCCACCGGCCACTTCCCCTACCCCTGCATCTACC CCTGCCCCTACCCCTGCCCTTGTCCTTAGGGAGCCTGCTGCTCCACCTGACCCCCATAGAGTTCTCAGAG GAGCATCAGTGGGTGTGAAAGGCAGGGAAGACCAGGCCACGACATGCTTTCCACCCAGCAGGCAGGAGCC CTGGGCACATTCAGGAACCAAAAACTTGCTTGTTAGAGAGTGTGAGGTCTTAGGAGAGCCTGCAAGGGGT AGTAAGACTCCATCTAAGGATGACTCTCTAGAGGAAGGAACACAAGACTTCTCTGAAGGCCAGTCCTCCT CTGAAGCTACCATGACAAGCATTTCTGGGAACAACAAAGCTGTGACCTCTGCCACCTGTCTCAGCTCACA GAAGGAACTTGGGACACCTGGAAACCTGAGGTATTCTCAAGGCCCCTTAAGACCAGGGCACAGAGGAAGT GCTCTTGATCCAGGGCCCATGCTCGTAGGCTGTGTGACCCATGTGGCAGCCCTGCAAATCTATCCAGACA GCAATTCTCCCAGACAGGATAAGGGCAATGGGCTCTTCTGGAAGCCTCAGGCTTGGGGTCCCAACATTCT GCAAAAGAACCCAATAAGCAGCAAGCCCAATGAAGCAGCTGGCTGTGGCCTCTCCTCCTCAGCCAGCCCA CAGGACCAGAAATGCCGTGACCTATTCCTGGACCTGAACCAGCTCAAGTTGGAACCCTCCAGGCTAGGCC CCCAAGCCTGTTCCTCTGTAGACAGCCAACCTCAGCAGCTCTGCCCCAGGGCTCCAGAGCAAGTGCCACA TAGGGGTTCAGTGGGGTCCTGAGCTACTCTGGGCCCCTGGTGCCCAGTCTCTGCGGCTATTGTCCAGAAG TCCTGTGGCTCTCTTTGGACAGTGAACAGCGGTAAGTCGCTCCTTCCCTTGGGCCATGAAATTTTTCGAT GGCCTTCTGACCTGATATGGACTTCATGTGGATAACCAGAAGTGTGGACTTACAAGCTGGCAGTGGCGCA GGCTCACCTACAGGATGGGCTCACACACTTCAGGAGCTGCATACGCATGGCCTCATTCAGGAGAACCAAG GACCAGAATCTTCATCTTTATTTATTTATTTTCTGGTGTAAGGATTGTGTTTGGGGTAGAGATGGCCAAG CTCAGGCATGTGGGGAGGAGGGGTAGAGGATCCCTGCCTGAGCCTGGGGCTAAAGACTGATGGGAGCTGC CGGCACCTCACACCACAAGGACAGACGACGGCCAGAAAGCAGTTCACGTTGTAAAGGAAGACAGGGGTAG CCAATCCATCGGCTTGAGGTACAGGAAAAGGATGAGAGCCGTGCACGGCTCAGGAGGCAGAACATCTCAT GAGGCTGAGTTCACAAGGACCCGCAGGTTTCATCCAAGTCTACTCTTTACCCAGGAAAGGAGAACAAGAT ACACCGCAGTGTCACAGTGCTTCTGTTTGGGAATGGCAAGGACCAGTCCTATCTTAGCAGGAGCCTGGGG CCTCCAGCATGGTATACGTGCACAGCTTTGCCAGGGCTGACCAGACCCTGCCTGACCAATAGGTTCTCTC CTAGTGATTCTGTGCACTTCCTTCAGCTTGTTCTCTGAGGCCTTGGGTGGGAGGATACCCACTGTTCTCC CCACCTCTGCCATTCTGCCTTGGAGTTGCAAAGGCCTTATCTTCTGCCCTTTCCACTCCCAGCGTGGCCA TCTCGCCTCCAGGGAGCTCTCTGAATGAAGCTGTGCTCACCACCCCAACCCAAAAGCAACACACGCACCG AACCCAGACATCCAGAAACAGGAAAGGACCTGCTGGCTGCTATCCCCAGGAAGGGTAGATATCATTTTTG ACCCAGCCTGAGGGCAGGGACAGAGGGTCCATCCTCCTTGTCAAGGATTTGATTCTACCTCAGCAAAAAA TAACACAGTGGCAGGCAGAGGATAAGGACTTGACAGGTAGCTGTAAAACTTGTCACCCGGACCATATGTA GGCAGGTTCCTGGAGAGGCCACACTTCATACAGAGCTCCTGTGGAGAGGTACAGGAACTACAGGTAAGCA CTGAAGTCCCATTGATATCACATGTTCAGGGCCCAAGGGCTCCACTTCAGGCGAGAAAGTCTCTTGTGAC CATTGGGACCAACAGCTCTCTAAGGCTGCCTACTGTGTGCGGATGAGGGCTGTAGGTTAAAAACAGATAT CAAGGTTGTGGCCATGTGGACCTGGACACAAGAAAGCACGGGGCCGTTCACCTGGGAAGCCACATCTTCT TCTAGGACCCAGACTCCATCATGACATCATAATGACATCACTGAGCCTGGCTTTGAGTAAGTGACAGATA GAAGAACATTCTGCATGACCCCTGCAGCAGTATGAGGTAAATTATGCTCATTTTCAGACCAGGCACAGCT TGTGTGATCTTTCTATGGACATACACAAGTAGTGAGTGCAGGTAGGGGGTTCAGATCCCTTCCAAACCCT ACTATGAAAGCTCTGCTATGCAGTAGGTTCTGGAACATTCCACTGGTTTCCCTCAGCTGATACTCCCAGA GCCTTCAGCCCTTTCTTCTTCCAGGCCACACATGCCTGAGCACCGTAGGGTGCCAACCACATGTCTCTGT GAACGGGTTGAAAGCTCAGTAAGGAACCACTCAACCCGTCACAGCCTTTGGGGCTCGGTACTGTACCTGA CAGAGAAAGTGCTCAGTAAATACTTTTTTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6853754 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853754 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13178 same embryo
	EMAGE:13181 same embryo
	EMAGE:13177 same embryo
	EMAGE:13179 same embryo
	EurExpress:euxassay_017223 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source