EMAGE mouse gene expression spatial database

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EMAGE:13218

Paqr8 progestin and adipoQ receptor family member VIII ( MGI:1921479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017230. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017230_01	euxassay_017230_02	euxassay_017230_03	euxassay_017230_04

euxassay_017230_05	euxassay_017230_06	euxassay_017230_07	euxassay_017230_08	euxassay_017230_09

euxassay_017230_10	euxassay_017230_11	euxassay_017230_12	euxassay_017230_13	euxassay_017230_14

euxassay_017230_15	euxassay_017230_16	euxassay_017230_17	euxassay_017230_18	euxassay_017230_19

euxassay_017230_20	euxassay_017230_21	euxassay_017230_22	euxassay_017230_23	euxassay_017230_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13218_wholemount_strong.wlz

13218_wholemount_moderate.wlz

13218_wholemount_weak.wlz

13218_wholemount_possible.wlz

13218_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13218_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9303
Entity Detected:	Paqr8, progestin and adipoQ receptor family member VIII ( MGI:1921479)
Sequence:	sense strand is shown >T9303 CGGAGCTGGAGTCGGGAGTGCGGCTGGCGGAGCTGGGCTGCAGGAGCTCGGCCGCGCTGGTGTTCGAGTT CCTCAGGTCCTGATTGAAGCACTTGAAGTAGCTTATCTGCCAGCTCTACCTCCCTGCTTGTTTGCAGAAA CCCCATGGTTCATCTCACACCTCAGCTGGAGCGTGGATTGCACATCTGTCATAATCCCACTACACGTCTC TCTATGGAACACTACAGACGCTAATCTCTACAAAGGTTGTCTAACTGAGCCCCGAGGAGCCCAGGGCCAC CGCTGTCATGACGACTGCCATCCTAGAGCGCCTGAGCACCCTGTCAATGAGTGGGCAGCAGCTGCGCCGT CTGCCCAAGATTCTGGAAGAAGGACTTCCCAAGATGCCATGCACCGTCCCAGAAACCGACGTGCCCCAGC TCTTTAGGGAGCCGTACATCCACGCGGGCTACCGCCCCACGGGGCACGAGTGGCGTTACTACTTCTTCAG CCTCTTTCAGAAGCACAACGAGGTGGTCAACGTCTGGACCCACTTGCTGGCTGCTCTAGCGGTCCTTTTG CGATTCTGGGCCTTTGTGGAGGCAGGGGCATTGCAGTGGGCCTCTCCCCACACCCTCCCCCTGCTCCTCT TTATCTTGTCGTCCATCACTTACCTCACCTGCAGCCTCTTGGCCCACCTGCTCCAGTCCAAATCAGAGCT GTCCCACTACACGTTCTACTTTGTGGACTACGTCGGGGTCAGCGTCTACCAGTATGGCAGCGCCCTGGCT CACTTCTTCTATAGCTCCGACCAGGCCTGGTATGAGCTGTTTTGGATTTTCTTCCTGCCGGCGGCTGCTT TCTGTGGCTGGCTCTCCTGCGCTGGCTGTTGTTATGCCAAGTATCGCTACCGACGGCCTTATCCAGTTAT GAGGAAGATCTGTCAAGTAGTACCAGCAGGGCTGGCCTTCGTCCTAGACATCAGCCCGGTGGCACACCGC GTGGCTCTCTGCCACCTGGCCGGTTGCCAGGAGCAGGCGGCCTGGTACCACACCCTTCAGATCCTCTTCT TCCTCGTCAGCGCATACTTCTTCTCGTGCCCTGTCCCCGAGAAGTACTTCCCCGGTTCCTGTGACATTGT GGGCCACGGACATCAAATCTTCCACGCCTTCCTGTCCGTCTGCACGCTCTCCCAGCTGGAGGCCATTCTT CTGGACTACCAGGGACGCCATGAGATCTTCCTCCAGCGCCACGGTCCCCTGTCTGTGTACAGCGCCTGTC TCTCCTTTTTCGTTTTAGCTGCCTGCAGTGCGGCCACCGCCACCCTCCTGAGGCACAAGGTCAAGGACAG ACTGATTAAGAAAGATTCCTGAGGTCCTTCAAATGAGGAAAGGTGTGGAGGTGGGCTACTGTGACTTGGA GAAAACTTGACCCAATAATAGAAGTTGACTTTTTATTTTTCAGGCTTGATTTTTTTTCTAAATTAATATG TATTACGGGCTGAGGATGAAACTCACATTGGTAGAGAGTGCTTATACAGCATGCAGAAAGCCCAGGGTTT GATTTCCCAGGGCCACAGAAGCCAGGGGGAGGCACAGGCAGGAAGATCAGAAATTCTAGGTCACCCTCTG ACATATAGTGAGTTCAAGGCTAGCCTTTGATACATGATAGATACCATATCTCAAACAAAATTATTATGTA TTTTCAAAGGCATGGTATGACTATGGAATTTCAAATGCAGGCCATTTGAGAGTTTTAAAACAGTGCGTTC ATTTTTCTGTCTGGGTGATAGCCTTGTTTGGGAAGAGATCTTGGCTAAGATTCATGAGACCCCAAACTAA GGAGAGCCATCTTCACCCATGAGCAATGAGCAGAATTCTGGTTGTAGCTTCCAAAGAGTAGTCCTGAAAC TAGACAGATATTTAAACTGGACTGCCAAGTATAACCTTGCCCTAACAGAACAGCCACCCTCCGCGCTGCT GGGCTTGGTGTCTGTGTAAACTGACCAACGAACTCTGTCTTATCAGGATTTCAGATTGCTTTGGGTGGAA AAAAACAGAATCTTCAAGAAGTTTCCTTGAAACTCTTAGACAAAATGGACTATTCAATTTATCATTCCTG GTCTCGACCACTGGTTCTTCTCTCAGGATAACCAACTAAGCTGGCCAATTTGCCTGAAGTACTACTGTTC TCATCCCTAGTCTGCATGGTAACAAAGAATCCCGAAGCTATTACCCTTCCAGGAAATGCTTGGAGCCATA TGAACATTCAGGAAGCTGATTCCCGCGTAATACAGAGGTATCGACAGATTTAACAGTGCCAAGAGCTGCC AAGAGACCCAGCCAGCAAGCACGGCTACCCTTGCCTGCCTGCTGCCCATGGGACCCTTGTAGTTTATATC TGAGGACAGCAGTATTAACTGCCAGCCACTTAGGGTTGTCTTGCAGGTCAGGAGATTGGTTGTCTGAAGG TTTGAAGAGACTCCTTTACAAGCATGGTTCTAAAGTTCTCCGAGAAAGCAGACTATGTCAAGCGGATCCT AAGATCCAAGGTTCAAGCTGCTGTTAGATGCTGCCCCGTGAAGGACCAACGCAGCTACCCCAGTTCAGAG TCCTCATGAGGCAGTTCCACCTCCGACTTGAGAAGAAACCTTAAAATGGGTCCTAGGAGGGGTGAATGTG TGCTCACCGTCGTTGGCCCCTGAAGGTCCCTTGAGTAAGAACGAGGCACCTGATGGCCGAAGGCACTGTC CGTGATATGGCTGCCAGCCCCTGATGTGAGGGGAGTGGTGCTTGCAGCTGAGATGTTCTGGCCACACAGC TCTCCCCGAGGACTGTCTTCTGCATGAACCCAGTGGAGGAGGCTGTGTTTGAGGAAGGGCTGAGGTGCCC TCTACTCGCAGGCTGCACTTCCTATCATTATAAGGTCCCTGTGTACTAAAAAAGGAAGCTAAAGTTTCCA ACTCCAGTTAATGAACCTCCTCTTGCTAGTCTTAAAACCTTTGTTTTCTATATATATATTTTTTGTGCGA GTGACTTGTGAATTTTGTGCCTTATACATGGGAATGTCTAAACTCTTTTATTTTAATATATGCACACATG GAATTTTTCTGTTTTGTGTTCTTGGGACAAGAAAAAAAAAGGAACCCCCAAAAGCTAGAGTGACCTGTCA ATCAACTATTAATCTGTATGTGAGAGGGGTCAGCAGCAGTATCCCTGCTGCTTTTAAATCAAGAGAGCAT GTGTCTCCTGCACTGTTTTATTAGATGGGGTTCTTGTTCATTCATGGTCAAGAAGATGAACGCCTATGCT AGCCAAATGACCCAAGGTTCTGATTGTCTAGGTCAAGTTCATGCCAAGGAATGTTAGCAAAACTGAACGG GTTTGGACTGAGCAAAGACTTCTTAGGGTGTGTATGTGTAAACGCTCCAAGTAGAGGGTCTGTTTAAAAT GGGAACCAGAAAGTGTTAGAAGTTCTGCTGTTGTGGATTCAAAGACTGTATTCTTTTAAAAGTTGGTGGG AAACGTGAGAGAGGAAAGGGATGAGGAAGGCGGTCTTCTGGTGGTATCTGTGAACAAAGATTTTTTTTTT TCCTTTTTCCTGAGATCAGATCTCACTGTATAACCCAGGCTGTCCTGGAAATCCTTATGTAGACCATGCA AACCTTGACCTCTAGAACTCTATAGAAATCAAGCTGTCTCTGCCTCCCACATGGCAGGGCTAAAGGTGTG GCATTCTGAGTCTTGTGGACAGGGATCTGTGGTATTCAATGGTCCTGCAGCCTAGGGTCGAGCTGAACTC TGTTCCTCACAAGTCTGTGACACCCGTGACACCCGTGACACCCGTGACGCCCGTGAGAAACGTGAGATAC TGGCGTTCAGTTCCTAGTTTAATTATACAACTATTGCTAACTATGGACTTGGGAACTGATGAAAAAGAAA AAGAAAGTGATTTCTATGGAGTTGGGACTTGAACCTAAGACCTGCAACATGTTAACAAAGAGTTCTCCCA TTGATGTGCACCCCAGGCCAAGCAACCGTGTATTTATAATAGATATTTGGGGAGGGCACTGAGGTGGGGG GAAAACACCAGTATCTAATTGGAATAATGCAAATGCCTTTACAAATGCATACACAAATGGGTGATTTTTA ACTCTTCAGAGTCCTCAAACAGTGTCATTAAAATGGGCCTATCAGGATGGTAAAGACTAGGCTAGTTTAA AAGTAACAACCAAAAATTGTAAAGAGGAGTATGCATGCCTCTTGATTTTACAATGGAGTTTGTCACCCTG CAAAATCAGCATAATGTTTAAAAAGGTATAAGGGAGATAGTTTATCTTGCTCTCCAAAACTAGAAATTTT GGTTGTTGCCTCTCTTAGTTAAGACTTTCAACCCATTAATATGTAAACGCCTCTGATAAGATTAAACCAT CAGTCATCTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6843180 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6843180 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13220 same embryo
	EMAGE:13215 same embryo
	EMAGE:13216 same embryo
	EMAGE:13217 same embryo
	EMAGE:13221 same embryo
	EMAGE:13219 same embryo
	EMAGE:13214 same embryo
	EurExpress:euxassay_017230 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source