EMAGE mouse gene expression spatial database

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EMAGE:13219

Olfr1443 olfactory receptor 1443 ( MGI:3031277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017329. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017329_01	euxassay_017329_02	euxassay_017329_03	euxassay_017329_04

euxassay_017329_05	euxassay_017329_06	euxassay_017329_07	euxassay_017329_08	euxassay_017329_09

euxassay_017329_10	euxassay_017329_11	euxassay_017329_12	euxassay_017329_13	euxassay_017329_14

euxassay_017329_15	euxassay_017329_16	euxassay_017329_17	euxassay_017329_18	euxassay_017329_19

euxassay_017329_20	euxassay_017329_21	euxassay_017329_22	euxassay_017329_23	euxassay_017329_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13219_wholemount_strong.wlz

13219_wholemount_moderate.wlz

13219_wholemount_weak.wlz

13219_wholemount_possible.wlz

13219_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13219_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9296
Entity Detected:	Olfr1443, olfactory receptor 1443 ( MGI:3031277)
Sequence:	sense strand is shown >T9296 CACTAAGCCACCTGAATGTTAATATTTTCCCAAACACACCGGTTTTCTTCTGAACTGGAGATTTTCTGAG AATCTTCAACCATGGTTGAAAAAAAAATACCCAAGGCTGTATTAATCAGCCTCATAGAAATGATCAGTTA GCAAGATTTCCTGTGTACCTTCCTCCACTTATATTCTACAAATACTGCTCTGTGGAATATATGAGAGAGG CAACATTATAATCTTTCTTTTACAGGCAAGGAAGAAGTAAGATACCCTTCTAAGGACACAGAGAGAGTTC ATAGTAAATTCATGGATGGACATGTTTGAGTGTCTTTTTTCTGTGTTTTTGTTGCTTTAGAAAGCTCCTA ACCTTTTGATTATTCCCTGTCAGAGAGACCCTCTGATGATACCTGTGAGTTACTCCACAAAAGGAATTCT TATTGCCAACTCTGTTCATAACTGACTGTCACTCTTGTTCAAAGAGCAAGATGATCCATTTGAGTGAAAA CAACAGCTTTTTAGTATGGATAAAACTACTTCAATATGGAATCATCACTCTTTGAACTAGTTATGGAAAA CTAGGCTATCAGGATAACTTCCTAATTACAAAATAACAGTGATGGAGAACAGGACAGAGGTGACAGAGTT CATTCTTCTAGGTGTGACCAATGCCCCAGCACTGCAGACCCCACTCTTTATCCTGTTCACTCTTATCTAC TTCATCAACATGACTGGAAACTTGGGGATGCTTGTGCTGATTCTCTGGGACTCTCGACTCCACACTCCCA TGTACATTTTTCTTGGTAATTTATCCCTGGTAGACATCTTTTACTCCTCTGCTGTTACCCCAACAGTTGT TGCTGGACTTCTTGTAGGAAACCAAGCCATTTCCTACAATGCTTGTGCTGCCCAGATGTTCTTGTTTGTA GTCTTTGCTACGGCAGAAAATTTTCTCTTGGCTGCAATGGCCTATGACCGCTATGCAGCAGTGTGCAAAC CCCTGCATTATACCACCACTATGACTCCAACTACATGTGCATGTCTGACCATGGCCTGCTATGCTGGTGG TTTCCTGAATTCCTCTATCCATACTGGGGACACATTCAGACTCTACTTCTGCAAGTCCAATGTGGTCCAT CACTTTTTCTGTGATGTTCCAGCCGTCATGGTTCTCTCTTGCTCTGATAGACACATCAGTGAGATGGTCC TCCTATATGGAGCAAGTTTTGTCATCTGTTCTGCACTCCTTGTTATTTTGATATCTTACATATTCATTTT TATCACCATCTTCAAGATGCGCTCAGCTGCAGGATACCAGAAGGCTATGTCTACCTGTGTTTCTCACTTC ACTGCTGTCTCTATTTTCTATGGCACTCTTATTTTCATGTACTTGCAGCCCAGCTCCAGCCACTCCATGG ACACTGACAAAATCGTGTCTGTGTTCTACACCATGGTCATTCCCATGCTGAACCCTGTGGTCTATAGCTT AAGGAACAAGGAGGTGAAAAGTGCATTCAAGAAAGTTGTGGAGAAAGCAAAATATACCTTAGGATTTTGA GTCTAGCATTGTGTGGTGCACTGTAATGCAGCTCCATGCTTCCCTAGGTATCCTGTGAGATTTCAGGACA CACTGACGTTAAGTACAAGAATCAATATCCTTATCTTTTCAAATGTTTGCTTTTCAGAAAATGGAAACAA TATTATAAATGCAATATCTGTTTTAGAATTGCTAACGTGAAGTTTAGCATGTGTTGGGTATTTTTTCCTA AATATTCATGGAGCAGACAATTTATTCACCATTTATACAAGCATTTTTTCTACATGCTACTAGTAACATA CATGTAAACTGAGAGGCAAAATAAAAGCCCATGAGAAGAAGATCTACTTGTGAACGCATGGGCATGCACA AATACAAAGAAAAAATATTCAAAGATAAAGGAACACTGAGCTATGGATGTTTTTGTTCTTGCTTTTTTGC ATTTGTGTATGGTATGTATGCTTTTATATTTGCATGTTTTTATATGTGAGGGTGTATGTGTGGATGCTGG TACACATATAGGGGCCAGAGGTTGGGTTTCTTTGTCATTAGTTCTCCACATAACTTATGGTAGTAGGCAA GTGAACACGACTTGGCAGGACAGGCTAGTCTAGCTAGTAACTTTTCTCTGAGATCCACCTCCATCTTCTG AGTTCTGGTATTACAGATGTAGTCCTATGGTCACAGTGCTGGTATTATAATGGGCCCCTATGTTGTCATT TATGTGACATTATATCATTATGTGGTCCATCACTTTTTCTGTTATGATCCAGAAGTCATGATTCATTCTT GCTCTGATAGCCACATCAGTGAGATGCAAACTTGTAAGCTTTGTTATCTGCCCTGCCCTCCTTGTTATTT TGGTATCACACATTTAGGAAAATTTAAGAGAATATGTGGTATTTATGAGAATTCCAACTTAGGTCTTCAT GCTTGTATAACAAGTGCTTTATCCTCTTATCTGTATTCTAGGTCACACTGCTAGTATTCAATAAGATAAG TTAATATAGCTCATCAGAGTATTGGAGTATCAATTATGATTGTTTTTGTTTAGTTGCATAACTATTCTAA GTCCTTTAATGGATGCAGGTTCAAACACACATGTCCATATTCTTGGTGTATTTATTGAGGGATAGAAATC AAAGTATTTTATACAAAATTAGCTTTTAAAAAGGTATATTAAGTGAATTAATATTTCTTTCTTGATGAAA CTAATGTATAGAAATTTATTTGTTATATTGTACATTTCTAAAGCAAAAATTATGCAGAGCATAGTGAAAG TGGAGTAAAAACAAAGAGATTTATTGCAGATGAACACCCTCATATGCACTGCTTTGAACATGCCCTGGGA CATTCATGTTTTCCATGTTCACTCATCACTAATAAAGTTGTGTCATCTCTTGTCACTCACAGTCTGTCCC CTCACCCCATTCATACTGATCCCCTTGATGGTCTTCCCAGCTGCACCCACATCCCTACTTCAGAAAACTT GAGCTTGCAGCTCCCCTGCATGGACATTTCCTTCACTACATTCACTTCTCTGCTTAAACAAGGGCTAGCC ATCTTGGGAGTCTTCCATGACTCCCATATAAGACAGTCACTTTCCTTCCTATTTACACTGTCCCTCCATT CTTCTTTGTGTTCACAATGCTCATGAGCACCTCAGCTGGAATCTAAATCTAAGTAAGAGCATTAGAGTAA TCTATTTTAACTACATGTGCCTGCTTGTTACCCATTCCTGGCATTGCTAATGTAGCCAAGAACCTGAGAC TAGATAGGTCATGGGTATAGGAGGAAAGGATTATTATTCTGCTAAAGGAACATAACTATAAAATGATTCC TGATGACATACATCAGGACCTTGATCAGCCCTCATCAAAACTGTTTCTTCTTGTAGTAAATGGCAATTTA CAGAAACCTGCAATTGAACAATGCAGAGAGTGTGAAAGACTTTGAAGCACTTAGACCTAAATGGGATGTC ATCATTACCTGCCTCTCCCCAGTGCTCAGAGATCTAAATAGAAGAGGAGGCAGAAAGATAACAAGAGCCA AAGGTGATGGATGACTTTCAGACACAACAGGACTGATGTACATATAAAGCAACAGTGACTATGGTAGCAT GCGAAAGGACTGCATATGTTCAAGCCACATGACATCCCAGCATTCTGAAGGGAAGAGGACACTAGTTCCC ACCCCTAACCCATAATCAGTCTGCAACTGATACCCACTGGAAAAGAAAATACCCATTTTCTTCAATGAAA TCTGACTGGATATATTAACAATATATCAGAGCATTCCTGATACTCAGTACTAGCTACCCAACACAAAACA AATTATTTCTTTTCTTCATCTTGTCTCTTGTTAAAATTCTCAGTTGTTTCACAGGAAGACCAACAGAATC AACTAACCTGAACCCTTGGGGCTTTCAGAGTCTGAACCAAAAGAACAACATAGTCAACCAAAGAACACAC ATAGCTTGACCTAGGCCTTCCTGCACATAAGTAGTAGATGCGCAGCTTGGTCTTTATGTGGGTCTTGAAC AACTGAAGTGGAGGGCAGGGGGTAGCTATCCCAAAAGCTGTTGCCTGCCTGTGGGATGTGTTCTTCTATC TGGGCTGGCCTTGTCTGACCTCAGTGGGAAAGGATGCACCTAACCTCACAGAGGCAGAACTGCCAGGGTG GGGGGGATATCCATTGCTCAGAGGAGAAATGGAGAAGGAGTGGGGAAAGATTGTGGGAGGTGTGACGAGG AGAGGGGCAGTGAGCAAGATGTAAAGTGGATAAGTAAAACAAATAAATATAATATAATATTTTTTAAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6841907 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6841907 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13218 same embryo
	EMAGE:13220 same embryo
	EMAGE:13215 same embryo
	EMAGE:13216 same embryo
	EMAGE:13217 same embryo
	EMAGE:13221 same embryo
	EMAGE:13214 same embryo
	EurExpress:euxassay_017329 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source