EMAGE mouse gene expression spatial database

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EMAGE:13221

Hdac7 histone deacetylase 7 ( MGI:1891835)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017319. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017319_01	euxassay_017319_02	euxassay_017319_03	euxassay_017319_04

euxassay_017319_05	euxassay_017319_06	euxassay_017319_07	euxassay_017319_08	euxassay_017319_09

euxassay_017319_10	euxassay_017319_11	euxassay_017319_12	euxassay_017319_13	euxassay_017319_14

euxassay_017319_15	euxassay_017319_16	euxassay_017319_17	euxassay_017319_18	euxassay_017319_19

euxassay_017319_20	euxassay_017319_21	euxassay_017319_22	euxassay_017319_23	euxassay_017319_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13221_wholemount_strong.wlz

13221_wholemount_moderate.wlz

13221_wholemount_weak.wlz

13221_wholemount_possible.wlz

13221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9156
Entity Detected:	Hdac7, histone deacetylase 7 ( MGI:1891835)
Sequence:	sense strand is shown >T9156 CGCCAGGCTGTGTGCTGCTGAGCGAAGCCCGGGTGGTGTGTCCCAGGGCCGCCAGCGCCGGCCCCCTCCC TCCCCTCCCCCTTGGCCCGCTCTCAGACTCAGATAAAGCATTTCCTTCCATTGTCATCCTACCCGGCCGG CCAGGCTGCCAGGGCCCTCCCCCTCCCGGCCCCCTCCCTTCCTCTCGCCGTCTCACAGTCGCTCTGCAGC CTCCGGCGACCGGGGGGATGTGAGGCCGGCGCCCCGGCCCCCCGCCCGCCATGAGCCCCCGGCTCTGAGG GCCCCGGCCCCTGGATGCACAGCCCCGGCGCGGGCTGCCCTGCCCTCCAGCCAGACACACCAGGCTCTCA GCCCCAACCCATGGACCTGCGGGTGGGCCAGCGGCCCACGGTGGAGCCCCCACCAGAGCCTGCGCTGCTG ACCCTGCAACACCCCCAACGCCTGCACCGCCATCTCTTCCTGGCAGGCTTACACCAGCAACAGCGCTCAG CCGAGCCCATGAGGCTCTCCATGGACCCACCAATGCCGGAGCTGCAGGGGGGACAGCAGGAGCAAGAACT TCGGCAACTTCTCAATAAAGACAAGAGCAAGCGAAGTGCCGTAGCCAGCAGTGTGGTCAAGCAGAAGCTG GCTGAAGTGATCCTGAAGAAACAGCAGGCAGCCCTTGAGAGAACAGTCCATCCCAGCAGCCCCAGTATTC CCTACAGAACTCTTGAGCCCTTGGACACAGAGGGTGCTGCCCGCTCCGTGCTTAGCAGCTTCCTGCCTCC TGTTCCCAGCCTGCCCACTGAACCCCCGGAACACTTTCCCTTGCGTAAAACAGTGTCTGAACCCAACCTG AAGTTGCGCTACAAACCCAAGAAATCCCTGGAGAGACGCAAGAATCCCCTGCTCAGGAAGGAGAGTGCCC CGCCCAGCCTTCGGAGGAGGCCTGCCGAGACCCTTGGAGATTCCTCCCCCAGTAGTAGCAGCACACCCGC GTCAGGGTGCAGCTCCCCTAATGACAGCGAGCATGGCCCTAACCCTGCCCTAGGCTCAGAGGCTGATGGT GACCGCAGGACCCATTCAACTTTAGGCCCTCGGGGTCCTGTACTGGGGAACCCCCATGCTCCCCTCTTCC TGCACCACGGTCTGGAGCCAGAGGCTGGGGGCACCTTACCCTCTCGCCTGCAACCCATTCTCCTGCTGGA CCCCTCAGTCTCTCATGCCCCACTGTGGACTGTGCCTGGCCTTGGGCCCTTGCCCTTCCACTTTGCCCAG CCCTTACTGACCACCGAGCGGCTCTCTGGGTCAGGCCTCCATCGACCACTTAACCGGACCCGCTCAGAGC CCCTGCCCCCCAGCGCCACAGCCTCCCCTCTGCTGGCCCCCCTGCAGCCCCGCCAGGATCGGCTCAAACC TCACGTCCAGCTGATCAAGCCAGCCATCTCCCCTCCCCAGAGGCCTGCCAAGCCCAGTGAGAAGCCCCGA CTGCGACAGATACCCTCGGCTGAGGACCTAGAGACAGATGGTGGGGGAGTGGGACCTATGGCGAATGATG GCCTGGAACATAGGGAGTCAGGCCGTGGGCCTCCTGAGGGCAGAGGCTCCATTTCTCTGCAGCAGCATCA ACAGGTGCCACCCTGGGAGCAGCAGCATCTAGCCGGGCGGCTCTCTCAGGGAAGCCCCGGGGACTCCGTG CTGATACCTCTGGCCCAGGTTGGACACCGGCCCCTGTCCAGAACCCAGTCTTCCCCAGCAGCACCTGTCT CCCTGCTGAGCCCAGAGCCCACCTGTCAGACCCAAGTCCTCAACAGCTCAGAGACACCTGCTACAGGGCT GGTCTATGACTCGGTGATGCTGAAACACCAATGTTCCTGTGGAGACAACAGCAAGCATCCCGAGCATGCA GGCCGCATCCAGAGCATCTGGTCCCGGCTGCAGGAACGGGGTCTCCGCAGCCAGTGTGAGTGTCTCCGAG GCCGAAAGGCTTCCCTAGAGGAGCTGCAGTCAGTCCACTCTGAACGGCACGTGCTCCTCTACGGCACGAA CCCACTCAGCCGCCTCAAACTGGATAACGGGAAGCTTACAGGACTCCTGGCACAGCGGACGTTTGTGATG CTACCCTGTGGCGGGGTTGGGGTCGATACTGACACCATCTGGAACGAGCTGCATTCCTCCAATGCAGCCC GCTGGGCTGCGGGCAGTGTCACCGACCTTGCCTTCAAAGTAGCTTCCCGAGAGCTGAAGAATGGCTTTGC TGTGGTGCGACCCCCGGGACACCATGCAGATCATTCTACAGCCATGGGCTTCTGCTTCTTCAACTCCGTG GCCATCGCCTGCCGACAGCTACAGCAACACGGCAAAGCCAGCAAGATCCTCATTGTTGACTGGGATGTTC ACCATGGCAACGGCACACAGCAGACTTTCTACCAGGACCCCAGTGTGCTCTACATTTCCCTTCATCGCCA TGACGACGGCAACTTCTTCCCAGGCAGTGGGGCCGTGGATGAGGTGGGAACTGGCAGTGGCGAGGGCTTC AATGTCAACGTGGCTTGGGCTGGGGGCTTGGATCCACCCATGGGGGATCCTGAGTACCTGGCTGCTTTCA GGATAGTGGTGATGCCCATTGCCCGAGAGTTTGCTCCAGACCTGGTCCTGGTGTCTGCTGGGTTTGATGC TGCGGAGGGTCACCCAGCCCCGCTGGGTGGCTACCATGTTTCTGCCAAATGTTTTGGGTACATGACGCAG CAGTTGATGAACTTGGCAGGAGGCGCCGTGGTGTTGGCCTTAGAGGGTGGACATGACCTCACGGCCATCT GTGATGCCTCGGAGGCCTGTGTAGCTGCTCTTCTGGGCAACAAGGTGGACCCCCTTTCAGAAGAAAGCTG GAAACAGAAACCCAACCTCAGTGCCATCCGCTCGCTGGAAGCTGTGGTCAGGGTGCACAGGAAATACTGG GGCTGCATGCAGCGCTTGGCCTCCTGTCCAGACTCCTGGCTACCCAGAGTGCCGGGAGCTGATGCAGAAG TGGAAGCCGTGACCGCGCTGGCATCCCTTTCTGTGGGCATCCTGGCTGAAGACAGGCCCTCGGAGCGGCT GGTGGAAGAGGAAGAACCCATGAACCTCTAGGGTTTCAGAACAGATCGCGCTTCAAATGTGGCTCTCCTC GTCTCTGATGTCAGCCCTGGTCCTGGGTCTTCAGAGATCCTCTGGGCAGGTAGTCAGGTCCAGAGCACAG CCTACCCTGACGGCTATCCCAAGGAACTTGAGAAGACCATGGGATCTGGGTAGGGGACCAGGGAGGACTC TGGGAGGAAGCAGGTTGGGCACTAGGGCACAGCTGGGCTCTTCCAGAACAGATTAGGCCGTCTGGTGTCC ACACCCCTAGCTGTGGTCTCCATCCCTCCCTCAGGACTGCACAGAGGGGGACTGGCTCAACTGGGCCACC CATAACCACTATTCTTAACTTGGCCTCGGCCAGGGTGGCCTTTCTCAGGTTCTGAAGGGCTAGGAGCTGG AGGAGCCAAGGGTCCCCTATTCCTGAGTACAGGAGAGTTTCCTCTCTCCTGTTTCCTCAAATGCCTTTCT GGTAGCTCTGGGCTCTGAGAGCCATTCTGAGCTGCTCTCTCCCTGTGAGTAGTAGTCACCTGCAAGTCTC CCATCAGTCACTGTCTCGCCAGAGGCCACCCTATGTGGACCATCTCAGTGCCCCGGTGCAGCAGACAGGT GCTCTCTGAGTCCTGTGTCTCCTAACCCAACGGTGCCAAACCTTCATCTCCCTTCAAAAGCACAACACAA TCCCAGGGATGCCTGGTCACTGTCCCCAGTATGAGCCTTCTGTTTCTGGGGTCCCTCCCACCCCTCAGCT CTGGCTGCTGCCCCACATAGGACTAGCTTGGCTGAGGGGAACAAGATGGGAGAGTAGACAGACATGGGGG AGGGGAGGCCACCCCAGAAGTCTTCGAGGCTTTTGAGGTTTAGGTCTGGGACCAAGAAAGAGTATTTGTG TGTATGTGTGTGTATGAGTGCGTGCAAGAGGGTATATGTGTGTGTGTCTTCCCACAACCCCACCGTACCT TGTCTATGTATGCATGTTTTTGTAAAAAAGAAAAAAGTGAAAAAAAAAAACAAACTTCTGAACAATAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6826982 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826982 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13218 same embryo
	EMAGE:13220 same embryo
	EMAGE:13215 same embryo
	EMAGE:13216 same embryo
	EMAGE:13217 same embryo
	EMAGE:13219 same embryo
	EMAGE:13214 same embryo
	EurExpress:euxassay_017319 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source