EMAGE mouse gene expression spatial database

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EMAGE:13400

Nedd1 neural precursor cell expressed, developmentally down-regulated gene 1 ( MGI:97293)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017529. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017529_01	euxassay_017529_02	euxassay_017529_03	euxassay_017529_04

euxassay_017529_05	euxassay_017529_06	euxassay_017529_07	euxassay_017529_08	euxassay_017529_09

euxassay_017529_10	euxassay_017529_11	euxassay_017529_12	euxassay_017529_13	euxassay_017529_14

euxassay_017529_15	euxassay_017529_16	euxassay_017529_17	euxassay_017529_18	euxassay_017529_19

euxassay_017529_20	euxassay_017529_21	euxassay_017529_22	euxassay_017529_23	euxassay_017529_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13400_wholemount_strong.wlz

13400_wholemount_moderate.wlz

13400_wholemount_weak.wlz

13400_wholemount_possible.wlz

13400_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13400_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9256
Entity Detected:	Nedd1, neural precursor cell expressed, developmentally down-regulated gene 1 ( MGI:97293)
Sequence:	sense strand is shown >T9256 GGGAGCCGGAAGCGCAGCGAGGAACGGGTGGAGCTGGGCCTCCCCTGTGGCCCCACGCTTGAGAGGCCCA GTCATGCAGGAAAACCTCAGATTTGCTTCATCAGGAGATGACGTTAAAATATGGGATGCTTCGTTCCTGA CATTGGTGGATAAATTCAACCCTCACACGTCACCGCATGGAATCAGTTCAATCTGTTGGAGCAGCAATAA TAACTTTCTAGTGACAGCTTCTTCCAGTGGTGACAAAATAGTGGTGTCAAGTTGCAAATGTAAGCCCGTT CCACTTTTGGAGCTTGCGGAAGGGCAAAAGCAGACATGTGTCGATTTAAATTCTACATCAATGTATTTGG CAAGTGGAGGCCTAAATAACACTGTTAATATTTGGGATTTAAAGTCCAAACGACTTCATCGGTCTCTTAA GGATCATAAATGTGAAGTAACTTGTGTGGCATACAATTGGAATGACTGTTACATTGCTTCTGGGTCTCTC AGTGGTGAGATTATCTTACACAGTGTAACCACTAATACATCCAGCACACCTTTTGGCCATGGGAGTAAGC AGCCTATCCGGCACATCAAGTACTCATTGTTTAGGAAGTCTCTCTTGGGCAGCGTTTCGGATAATGGAGT AGTGACTCTCTGGGATGTGAACAGTCAGAGTTCATACCACACCTTTGACAGTACACACAAAGCCCCAGCT TCAGGCATCTGCTTCTCTCCTGTCAATGAACTGCTGTTTGTAACCATAGGCCTGGATAAAAGAATCATCC TCTATGATACTTCAAGTAAGAAGCTAGTGAAAACTTTAGTGGCCGACACCCCCCTGACTGCAGTAGATTT TATGCCGGATGGAGCCACTTTGGCTATTGGATCTTCCCGTGGGAAAATATATCAGTATGACTTAAGGATG CTGAAATCTCCCGTGAAAACCATCAGTGCCCACAAGACATCTGTGCAGTGTATAGCATTTCAGTACTCCA CATCTCTGACGAAGGCAAGTTTAAGCAAAGGCAGCTCAAATAAGGCCACAGCAGTGAACAAACGGAGTGT TCCTGTGAGCAGCAGCAGTGGGGCTGCCCAGAATTCTGGAATTGTCAGAGAAGCACCCAGCCCTTCCATT GCCACAGTTCTCCCACAGCCCGTGACAACAGCTCTGGGCAAAGGATCAGGGGCTGCTCAGGACGAAGCAG GTTTGGCTCGAAGCAAAAGTACAGATATTTTCTCTAAGGAAACAGATGCTGGAAAAAGTCAGGATTTCTC CAGCTTTGATGATACTGGGAAAAATAGTTTAGGTGACATGTTCTCACCAATCAGAGATGATGCTGTAGTC AGCAAAGGAGGTGATGAATCTATAGGCAAAGGAGATGGCCTTGACTTTCTACCACAGCTAAACTCAGTGT TCCCTCTGAGGAAGAATGCAGGTGCCTCCAGCAGTCTGGTGCTGCACTCCAGCCCTCTTAATGTCCTCAT GGGATCTTCAGGGAAAGAAGAGAATGAAAGCCATGAGCCGTCAGCTGAGTCTAAAAGAGCATACCTAGGG AAGCAGGAACCTAAGGACGCCATGAAGCAGTTTACAAAGTTGATCTCCTCTGGTGCTGAACCTGGAATTC TAAATACCTGTCCATCGTCTAACCAAGCAAGAAATCTTGAAAAATTTGAAAAGCCAGAAAAAGATATTGA AGCCCAGTTGATACATGAACCCTCAGTCAACGGCTCCTCAACTACAGTACCAAAGGCAGCATCCTCCGTC ACTGCTGGAGTGGCCAGTTCACTCTCAGAGAAAATAGTCGATACCCTTGGGAACAGTCGGCCAGGGGCGC CGCTTACCTCAGTCCAGATCCGCTTCATTCAGAACATGATACAAGAAACACTGGATGACTTCAGGGAAGC TTGCCATAGAGACATTGTGAATCTGCAAGTGGAAATGATTAAACAGTTTCATATACAGCTGAATGAAATG CACTCTTTGCTGGAAAGATACTCCGTGAATGAAGGCTTAGTGGCTGAGATCGAAAGGCTGCGAGAAGAAA ACAAAAGGCTGCGGGCCCATTTCTGAAGACTCTGGGCACCTTGATTTGTTATGAGTTAGGATGTTTCTGG CAACTATAATACACGGAATCAGTATTGTTTGCATGGCCTTGAAAGTGGATTTTGCAGCACGCACTATTCC ATCTCTGTCAGACATTTATATTTTTATATTAAAACTGCACAGAATACCAATCTTCCTAGTGGGGAAGTCA ACAGGATCTTTATTTTCTGAATGCCTTAGCTATACACTAAGTGCCCTTTTCCTAAAAGAAGAAAATGTTC ATAAGAATAGGTTTATGTTTTGTAAATGACTTTTAAAAATAGATATATTTTTGATGGACAAATTGTATTC CAAATTTTTTTAGGTAGCCAACTTTTAAAACCTCGGTATACCTTCTATTTTATAAAGAAATAATTGTAAA GTGGCAATTGGATTTTTTCACAGTTTAGTAATTATTTTGTTAACATGATGACATCAGGTATGCCTTATTT ATTTAGTGTATATGTAATACATTTTTGAAACTTTGCATTGTACAGATACATGGTACCTTTGTTCTGGTTA CCTAATTTTATATATATATAATTTTTTTAAGGGTTTGGAAGATTCAAGGGCTATATATCTGCTGATTGTC TAATGAATTTACTATGTGCAAAATCATTACTATAAGATTTTCATCACTCAACTTAGGATCAGTGAAATTT TCTTATACTAACTAAATATTAGAATATTGTTGCCATTTTTTCCCTCACAAGTAACAGGCATAGATTATTT CTTCTGTGTTGTGTATCATTAACTTTCTCTAATTACAGTGTGCTGTGAACAGCACTTTCTATCCTTAGCC TTACCATAATATTTATAAAGGTTCATCATGCAGAGAATGTACATGTTGGCGGAGCTGGCCGTGTTCATCT GCCTGCTGGAACCTACACTTTGATAGGGCTGGACTAGGGGCTACTCACCGTTTATATTCTTGTCGCCTCT TGCCCAAAGATCTCAACTGTGTACTTTATATGTACATCTGTTCCCCACTTTTGCTTTCCAGGATCAAGAT GTTTACTAAAATGTAGCTTAGATTGATAACTTTAAGTGTTTCTAAAGAGATAGAATCTGTAAGGGTGTAT TTAATAAATATAAACCTATGAATAATTATCTCCAAAAGTGGAACAGGAGTAAGACTTTTCCAAGTGACTC AGAAAGTCTGTTTGTGTGTCAAGTTTCTGTGGCCTTTTCTTGGCAGCTATGGCCGCATTATGACTTGTAA CAAGTCCCCTTACATGTCACTCAAACAACGTTAAAATGCAGACGTCTTTTGTCTATATACTACATTTTAA CCTATTAATATTTTCCTATTTCCTTTTTTCTATAGGTGTATATATTGTGTATTAAAAAGGGTCTGATAGA GCATGTATATAGGTAAAACACTTGTCATGGATTTAAGCACTGAGTACAATAAATTTTCACATTCAAAATG TAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6836583 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6836583 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13402 same embryo
	EMAGE:13401 same embryo
	EurExpress:euxassay_017529 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source