EMAGE mouse gene expression spatial database

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EMAGE:13401

Tshr thyroid stimulating hormone receptor ( MGI:98849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017542. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017542_01	euxassay_017542_02	euxassay_017542_03	euxassay_017542_04

euxassay_017542_05	euxassay_017542_06	euxassay_017542_07	euxassay_017542_08	euxassay_017542_09

euxassay_017542_10	euxassay_017542_11	euxassay_017542_12	euxassay_017542_13	euxassay_017542_14

euxassay_017542_15	euxassay_017542_16	euxassay_017542_17	euxassay_017542_18	euxassay_017542_19

euxassay_017542_20	euxassay_017542_21	euxassay_017542_22	euxassay_017542_23	euxassay_017542_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13401_wholemount_strong.wlz

13401_wholemount_moderate.wlz

13401_wholemount_weak.wlz

13401_wholemount_possible.wlz

13401_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13401_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9466
Entity Detected:	Tshr, thyroid stimulating hormone receptor ( MGI:98849)
Sequence:	sense strand is shown >T9466 CAACGATGAAGTAGCCCAGAGGGTCCCTTGGAAAATGAGGCCAGGGTCCCTGCTGCTGCTTGTTCTGCTG CTCGCCCTGTCCAGGAGCCTGCGGGGCAAAGAGTGTGCGTCTCCACCCTGTGAGTGTCACCAGGAGGACG ACTTCAGAGTCACCTGCAAGGAGCTCCACCGAATCCCCAGCCTGCCGCCCAGCACCCAGACTCTGAAGCT CATCGAGACTCATCTGAAGACCATACCCAGTCTTGCATTTTCGAGTCTGCCCAATATTTCCAGGATCTAT TTATCTATAGATGCAACTCTGCAGCGGCTGGAACCACATTCTTTCTACAATTTGAGTAAAATGACTCACA TAGAAATCCGGAACACCAGAAGCTTAACCTATATAGACCCTGATGCCTTGACAGAGCTCCCCTTGCTCAA GTTTCTTGGCATTTTCAATACTGGACTTAGAATATTCCCTGACTTGACCAAAATTTATTCCACGGACATA TTCTTTATACTTGAAATCACAGACAACCCTTACATGACTTCGGTCCCTGAAAACGCATTCCAGGGCCTAT GCAATGAAACCTTGACCCTGAAACTGTACAACAATGGATTTACTTCAGTCCAAGGACATGCTTTCAATGG AACAAAGCTGGATGCTGTTTACCTAAACAAGAATAAATACCTGACAGCTATAGACAACGATGCCTTTGGA GGAGTATACAGTGGACCAACTTTGCTAGATGTGTCTTCCACCAGCGTCACTGCCCTTCCTTCCAAAGGCC TGGAGCACCTCAAAGAACTGATCGCAAAAGACACCTGGACTCTCAAAAAGCTCCCGCTGTCGTTGAGTTT CCTCCACCTCACTCGGGCTGACCTCTCTTACCCGAGCCACTGCTGCGCTTTTAAGAACCAGAAGAAAATC AGGGGAATCCTGGAGTCTTTGATGTGTAATGAGAGCAGTATCCGGAACCTTCGTCAAAGGAAATCAGTGA ACATCTTGAGGGGTCCCATCTACCAGGAATATGAAGAAGATCCGGGTGACAACAGTGTTGGGTACAAACA AAACTCCAAGTTCCAGGAGAGCCCAAGCAACTCTCACTATTACGTCTTCTTTGAAGAACAAGAGGATGAG GTCGTTGGTTTCGGCCAAGAGCTCAAAAATCCTCAGGAAGAGACTCTCCAAGCCTTCGAGAGCCACTATG ACTACACGGTGTGTGGGGACAACGAGGACATGGTGTGTACCCCCAAGTCGGACGAGTTTAACCCCTGTGA AGATATCATGGGCTACAGGTTCCTGAGAATCGTGGTGTGGTTTGTCAGTCTGCTGGCTCTCCTGGGCAAT ATCTTCGTCCTGCTCATTCTGCTAACCAGCCACTACAAATTGACCGTGCCGCGGTTCCTCATGTGCAACT TGGCCTTTGCAGATTTCTGCATGGGGGTATACCTGCTTCTCATTGCCTCTGTAGACCTGTACACACACTC TGAGTACTACAACCACGCCATCGACTGGCAGACGGGCCCTGGGTGCAACACGGCTGGCTTCTTCACTGTT TTCGCCAGTGAGTTATCAGTGTACACACTGACGGTCATCACCCTGGAGCGATGGTACGCCATCACCTTCG CCATGCGCCTGGATAGGAAGATCCGCCTCAGGCACGCGTACACCATCATGGCTGGGGGCTGGGTTTCCTG CTTCCTTCTCGCCCTGCTCCCGATGGTGGGAATCAGCAGCTATGCCAAGGTCAGCATCTGCCTGCCAATG GACACCGACACCCCTCTTGCACTCGCATACATTGTCCTCGTTCTGCTGCTCAATGTTGTTGCCTTTGTTG TCGTCTGTTCCTGCTATGTGAAGATCTACATCACGGTCCGAAATCCCCAGTACAACCCTCGAGATAAAGA CACCAAGATTGCCAAGAGGATGGCTGTGTTGATCTTCACTGACTTCATGTGCATGGCGCCCATCTCCTTC TATGCGCTGTCGGCACTTATGAACAAGCCTCTAATCACTGTTACTAACTCCAAAATCTTGTTGGTTCTCT TCTACCCCCTCAACTCCTGTGCCAATCCGTTTCTCTATGCTATTTTCACCAAGGCCTTCCAGAGGGACGT GTTCATCCTGCTCAGCAAGTTTGGCATCTGCAAACGCCAGGCCCAGGCCTATCAGGGTCAGAGAGTCTGT CCCAACAATAGCACTGGTATTCAGATCCAAAAGATTCCCCAGGACACGAGGCAGAGTCTCCCCAACATGC AAGATACCTATGAACTGCTTGGAAACTCCCAGCTAGCTCCAAAACTGCAGGGACAAATCTCAGAAGAGTA TAAGCAAACAGCCTTGTAAAGGAAAGGCTACGCTAGTCACAGTGAGACTTACAAAAGGCTGGTTTCTTGA ACATGCGTTCCAGTCCCGTGACATGTGAACACATAGGTTCATGCAGGTGATGATTCATAGGGTCAGAGTT CATCTCTAGAAAGTATTGCCTCTAAGAAGGAAGAGACGCTATCAGCATATCTGAGTCCTAGGTGATATGA CAATCGCCTATGCTCGCTGGGAGATTTGCTTGTTGCTTGTCTATGATGCCATGCAACATGTTCCACAGAC ACCAGTTGAACCATTCCCACATCTTGCTTCTCCATTTTATGTAGTGTTTTATACTAAAGACTCAACAACT GGCCAGTGTTCATCATACTCCATATTAGCACCCCTGAAAGAACCAAAGAAATGTTTTTATTCCTTAAAAA TAAAATGCCAAGGTAAAATCTGGACTGTTAATAAAAAAAGATTAAATAAAATTAAATAGATAAATAAAAT GCCACACTTGGAAGGTGGAGGTAGGGGAATCCAGTTCATACTTAGCCACATAGAGAATTCCAAGCCAACT TGTGCTACCTGAAGCTCCACCTCAAGCTACCAAAAGAAGCCTGGAAAGCCAAACATATAGATGTCACATA ATTGCTTTTATTAAACTGAAAAAAAGAGCACGTCTAGTTTTGAGAGGTTTTTCTTTGTTTTTCTTCCTTA GACTCTGAATCAATCATCACCAGGCTCTGCCTGCCGTGACCCAGCTGTTACACGCTGACTGGAGAAACTG CCAAGGTTTCAGCTTTTGTGGCTGAGCAAACTTAAGAATTATCTTCTCATATAAAACATGAGCTCCGGGA GAGATTTCTAAGGACCACATGGGAATGAGGACAGGGCACACTTGGCCACGTGTTGAATAAGACAACTATA TACCAGTTAACTTGTCGGGCTTTGAGCAAGTCTCTGGACCTCTAAATTCTGTAGAAATGGGGGGATTTGA TGCCCTCTTCCATTAAAAAACAAAAACAAAAAAACCTAGGTATATATCCCCCTCAAAAGATATGTGTGAC AAAGACAAAGAAAACACTACTTAAGTAGACTCGGTTTTTTCTACCTTGTGACGCTGCTGTCTACTTCTTT TAGGAACCAGGACATGTGACCCAAGATGCTTTTTCTTTATTTCATTCTGATCATCGTGTCTGAACCAAAA GTTCTATTGAGTGACTGACTATGTGGAGCCAGATCCTAACTATTCATCTAAATTATTGTATCTATACTAC TGTCAAGTCCAAGACATACTGGAATTTATATAAACTGTTAATAATACAGCCCAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:30650002 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30650002 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13402 same embryo
	EMAGE:13400 same embryo
	EurExpress:euxassay_017542 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source