EMAGE mouse gene expression spatial database

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EMAGE:13421

Klf11 Kruppel-like factor 11 ( MGI:2653368)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017541. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017541_01	euxassay_017541_02	euxassay_017541_03	euxassay_017541_04

euxassay_017541_05	euxassay_017541_06	euxassay_017541_07	euxassay_017541_08	euxassay_017541_09

euxassay_017541_10	euxassay_017541_11	euxassay_017541_12	euxassay_017541_13	euxassay_017541_14

euxassay_017541_15	euxassay_017541_16	euxassay_017541_17	euxassay_017541_18	euxassay_017541_19

euxassay_017541_20	euxassay_017541_21	euxassay_017541_22	euxassay_017541_23	euxassay_017541_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13421_wholemount_strong.wlz

13421_wholemount_moderate.wlz

13421_wholemount_weak.wlz

13421_wholemount_possible.wlz

13421_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13421_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9344
Entity Detected:	Klf11, Kruppel-like factor 11 ( MGI:2653368)
Sequence:	sense strand is shown >T9344 GCCGCGCGGAGGCAGAGCCGCGCGGCAAGGAAGGCGGAGGTGCGCGCCGGGAACAGACGCTCGGGACACC GCCGCCCGCCGCACACGTGCAGCCTCCGCCGCACGCAGGGCTCCTGCCTCGCAGCATTGCTGCCTGCAGG CCATACGGCACCATGCACTCGCCGGGCTCCACCGGCCCCGGCGACGGGCGCGCGGCTGACATCATGGACA TTTGTGAGTCGATCCTGGAGAGGAAGCGGCATGACAGCGAAAGGTCCACATGCAGCGTCCTGGAGCAGAC GGACATCGAAGCTGTGGAAGCTCTTGTCTGCATGAGCTCTTGGGGTCAGAGGTCCCAGATGAGACCCCTC ACGCCTGTCTCTGACTCTGGTGACGTCACCACTGCTGTGCTTATGGACACAGCTGCACCTGATCTACCAA AGGACTTCCATTCTTTTTCAACTCTGTGTATAACTCCTCCTCAGAGCCCAGAGCTCACGGAGCCGTCAAC AGGGACCCCTGTTCCTTCCCAAGTAGTTAACTCCAAAGGATGCATGGTCACTGCCCTCCCCCCATCCCCT GCTGGGGGCCCCAGAACACTGAGCAAGAGGGAACCACTGGAGCCAGCCTCAGGGTCCTCCTGCAGGGCTG TGATGACCAGTGTGATCCGTCACACCGGGGAGAGTCCTGCTCCTACGCGCTTCCCAACTGGCCCCACTCA AGAGCAACGAGCATCCGACAGCGGAGAAGGACAGGAGCGTTTGCTGGACCACCTCGAAGCCTTGCAGGAC ACACGCTTGGCAAATGGCTTACTCGTCACTAACTTGGTTTCCTGTCAGCCTTGCTTGCACAAGTCCGGTG GTTCGTTCCCCACTGACAAAGGTCAACAGACAGGATGGCCCGCTGCTGTTCAGACCTGCTTACCAAAGAA TCCTGAAAGTGACTTGTCAAGGAAAATCACCCCTCTGATTTCTGTCCCTGTATCCAGTCCCCCTGTCCTT TGCCAGATGATCCCTGTGGCTGGACAGAATGGCTTGTTCTCAGCCTTTTTGAAACCTCCTACCCAGTTGC CTGCAGGGACTATCAAGCCCATCCTACCCCAAGCTGCTTCAATGTCTCAGCCTGTGTTCATGGGCCCACC TGTGCCTCAGGGAACTGTGATGCTGGTCTTGCCACAGAACACCTTCCCCCAGCCTGCTGCCTGTCCATCC AGTGTCATGGCCATTGGAAATACCAAGCTGTTGCCCCTTGCCCCTGCACCAGTGTTCCTTGCCTCCAGCC AGAACTGTGCCCCTCAGGTAGACTTTTCCCGAAGGAGGAACTATGTTTGCAACTTCCCAGGATGCCGGAA GACCTACTTCAAAAGTTCCCACCTTAAAGCTCATCTTCGCACTCACACAGGGGAGAAGCCTTTTACCTGC AGCTGGGACGGCTGTGACAAGAAGTTCGCAAGGTCAGATGAACTGTCTCGCCACCGCAGGACTCACACGG GAGAGAAGAAGTTTGTGTGTCCTGTGTGTGACCGGCGCTTCATGCGGAGTGACCACCTGACAAAGCACGC TCGTCGCCACATGACAACCAAGAAGATCCCAGGCTGGCAGGCAGAGGTGGGCAAGCTGAACAGAATCACT TTGGCAGAGAGCCCCGGGAGCATCCTCGAACCCTTGCCGGCCTCTGGCTGAGGCATCCTTCTGTGGCATC CGTCACCTGTGGGCTTCTTCAAAAGCCTCTTCAGGAATGGAACCTGTGTGTTCCTCTCCAAAAATAAATG TGGTCTCTGGGCTGTTGAAGTGGGGAGCTGGTTCTGATGGAAGTATATTAATTTTACTTTTCTGGTTGGG ACCCTGTTTGATATCTTTTGTAGTTTTAGAACTCTGACTGTCTTTGTCTGTGTGTGTGTCTCTGTGTGTG TGTCTCTGTGTGTGTGTGTGTCTGTGTCTGTGTGTGTCTGTGTCTCTGTCGTGTCTCTGTCTCTCTCTCT CTTTTGTAAGGAAATGGAAGAAAATTTGCTCTAAATCACCAGCATTCAAACAAATATTTCTGCAATAAAG TTTACAAAGTCTGGATTTTTTTTTATACCTTTCTATTCATGTTTTGTAGAAATGTGACAGGGTACTAATT TTTATACTTTTTATAAAAATATTTATATTGTGCTCAAATCACCAGTTTGTGGATAGAAAATTTTACAGCC TTCTTTTCCATGTTTTATAACAAAGTTTTGTAATAACCCTGCTTAAGAGTAAGCCTGGTAGTTAGTCAGA AAGAAGCAAAAATAACAAGTTTGTTACCAGCCTTTTATAGTCAATATTTTAGATCAGAACTGGTTGAATA AAGAACTCTTAAAGGGGAAAAATAAACTTTGTAGTTAGCTTCTTTGTATCAGTTTCAGGGGAAATTCTAG CAATCGATCCTATAGCCCACAGAACTAAGATTGTGGTTAGGTAAAACTAATCATGTGAGTCTCGTGTCCC TGTGGAAGGTGTGCCCAGTCTGCCTGGGCTGCTGGCCAGCTGAGCAGGGGCAAATATGGAATGTGGACTC CTGGGCCATCTGTCTGCTAAGAGCAAATGACCCCATGTCCTGTCTGTGTTAAGCACCAACACTAAGAATG TAGACGCAGAAACTCTGTATGTTACCTGTTGGCTTCTGTTGTGGTTTTGTACTGCGTGCTAGATTGAATG ACCACAGGGAAAAGGAAATGTCTGTACTGGTTGGTTAAACTCTCCGCCAGAGTGCAGCTTCCACAAAGCA AGGCCTTTGTGCACATTCGGCTCTGGGGTTCGGATGCCTCTCTGACTATGGACAAAACACTGAGAAACAT ATCATTAAATGGCAGTGGCTGTCACCCAACTGCTTTTTCAATCATTGTGTGTGAAGGAAATTAGTTTGCT CAAACAAGAAGACTTGGAATGGGTATATTGTAGCTTTCCTGAAAACAGATTTCAGGCACTGCTTTTACGC CTCGTGAGCGGGTGTTCTGCATCCAGGTGATCACAAAAACAGAATGGTTTTAGATAAATTCTGGGAAGCT CAGGCAGTACAGCAAGTGTGATAACAGGTATATTGTGCTGTTTGGGCAGCCTGAGACTATTTTTGTGTGG TATGTATTAGGTTTTGTATTTGCCTTTTAATCATTGGTATTTAACTTTATTAAATGATGGCAAGGTTGAA ATAATTAAACGTAAAATGAGGCTTTTGTGTTCAGGTCTTTTGAGCCTTATATAACCGTTATGTGGTCATA CCTTGTATGTTACTTGACCCCTGTTAAGCTGCTGTGGTAGCCGAGTTCTGTATGTACATGTGTTTAGGAA CTGCCCAGACAGCAGTCTTGCTAATGTCTTTCAACATAATGGAGTGCAGGAAAACTAACTGTATATAGCT CACGTGTGTCCCAGCACCCTGGTCCTGTCCACCTGTTTTAAGTCAGCATCCACACTGGCGACCGTGTAAG TGATAAATGGCATCCAACTTGGATGTCTTTTTTCATTGTGACATGCAGAGTGCTGTTCCTTGTCCTGGCA TTTCTGTGGGTAGCGGAAGGCCTTGGGGTGTTTGGTTGCTCATCACAGGACTTTATCTGACCAGTGTTGT TTCTAAATGACTGGTGTGTTGCAATGCCATTAGGTTTCCATTTCCCAATTAGATTTAGAAACCCCTTTTC TGAAAGAGCCCCCAACATTTGTCTACTGAAAGACTAAGTTGGAAAACAGTTCCTATCTGAAAAGGACCCC TGAGCCCCAGAGCCCCGGGAGTGGCTGCTCCCTTGAGGGCCCTCCCCAAATCCCCACGGCGTGGTGGTGA CCCCAGTGCACTTTATCGCTTGAGAAGTTCTTGATTTTTTTTTAATTATTTTGTTGAGCCTTTCAGTAGT TTTAGTAATAAAAGTTTAAATGTTAACAACTTTTGTTTTATGTAAAAGACTTTGACTTATTTAAATGAAC ATGTGTAATGTAACTTTTTTTTTTCTTTGAATCGTATAAAATTCTCTTGGTTCGCAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6850350 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6850350 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13419 same embryo
	EMAGE:13420 same embryo
	EMAGE:13417 same embryo
	EMAGE:13418 same embryo
	EMAGE:13413 same embryo
	EMAGE:13414 same embryo
	EMAGE:13415 same embryo
	EMAGE:13416 same embryo
	EurExpress:euxassay_017541 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source