EMAGE mouse gene expression spatial database

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EMAGE:13423

Rbm12 RNA binding motif protein 12 ( MGI:1922960)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017452. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017452_01	euxassay_017452_02	euxassay_017452_03	euxassay_017452_04

euxassay_017452_05	euxassay_017452_06	euxassay_017452_07	euxassay_017452_08	euxassay_017452_09

euxassay_017452_10	euxassay_017452_11	euxassay_017452_12	euxassay_017452_13	euxassay_017452_14

euxassay_017452_15	euxassay_017452_16	euxassay_017452_17	euxassay_017452_18	euxassay_017452_19

euxassay_017452_20	euxassay_017452_21	euxassay_017452_22	euxassay_017452_23	euxassay_017452_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13423_wholemount_strong.wlz

13423_wholemount_moderate.wlz

13423_wholemount_weak.wlz

13423_wholemount_possible.wlz

13423_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13423_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9229
Entity Detected:	Rbm12, RNA binding motif protein 12 ( MGI:1922960)
Sequence:	sense strand is shown >T9229 GAGGTGTTGGCTGCAGTGGGCTCCGGGAAAGCCGTTCGGGCTGGGGCTGTCGGCCGCAGGGCTGAGGCAC TCGCGCGGGGGGTAACTCGGGTCTGGGTTCTGGTGCCGCGCAAGCTCTCCCCGTCTAAAAGTTGGTTTTA ATTGGTTGCCCACAGGATTGACTTGGCCTCTGCTTCTTAAGAAAATACATCTCTTGTTTTACAGGTGTGT GTGGTTTCAGCGCAGCATGGCTGTGGTCATCCGTTTGCAAGGTCTCCCAATTGTGGCGGGGACCATGGAC ATTCGCCACTTCTTCTCTGGATTAACCATCCCCGATGGGGGCGTGCATATTGTAGGGGGTGAACTGGGTG AGGCTTTCATCGTTTTTGCCACTGATGAAGATGCAAGGCTTGGTATGATGCGCACAGGTGGTACTATTAA AGGGTCAAAAGTAACACTATTATTGAGTAGTAAAACAGAAATGCAAAATATGATTGAACTGAGTCGTAGG CGTTTTGAAACTGCCAACTTAGATATTCCGCCAGCAAATGCTAGTAGATCAGGACCACCACCTAGCTCAG GGATGAGCAGCAGGGTGAACTTGCCAGCAATAGTACCCAACTTTAATAATCCTTCGCCAAGTGTAGTAAC TGCTACCACTTCTGTTCATGAGAGCAACAAAAACATACAGACATTTTCCACAGCCAGCGTAGGAACCGCT CCTCCAAGCATGGGGACTTCTTTTGGGAGCCCAACGTTCAGTTCAACCATTCCAAGTACAGCTTCTCCAA TGAACACGGTCCCACCGCCACCGATTCCTCCAATCCCAGCGATGCCATCTTTGCCACCGCTGCCATCCAT TCCCCCAATACCAGTTCCTCCTCCGGTACCAACATTGCCTCCTGTGCCACCTGTTCCTCCCATTCCCCCA GTCCCTTCGGTGCCACCTATGACCACACTGCCTCCTATGTCAGGCATGCCACCCTTGAATCCACCACCTG TGGCACCTTTACCTGCTGGAATGAATGGCTCTGGAGCACCTATCGGTCTGAACAATAATATGAATCCTGT GTTTCTGGGTCCATTAAATCCTGTTAACTCTATACAGATGAATTCTCAAAGCAGCGTGAAGTCTCTTCCC ATCAACCCTGATGATCTGTATGTGAGTGTGCATGGAATGCCCTTTTCTGCAATGGAAAATGATGTCAGAG AGTTTTTCCATGGGCTCCGAGTCGATGCAGTGCATTTGTTAAAAGATCATGTAGGTCGAAATAATGGGAA TGGATTGGTTAAGTTTCTCTCCCCTCAAGATACATTTGAAGCTTTGAAACGGAACAGAATGCTGATGATT CAACGCTATGTGGAAGTCAGTCCTGCCACAGAGAGACAGTGGGTAGCTGCTGGAGGTCATATCACTTTTA AGCAAAGTATGGGGCCTTCTGGACAAGCCCACCCTCCTCCACAGACACTTCCAAGGTCAAAATCGCCCAG TGGGCAGAAAAGGTCAAGATCAAGATCACCACATGAGGCTGGCTTTTGTGTTTACTTAAAAGGGCTACCA TTTGAAGCAGAAAACAAACATGTCATTGATTTTTTTAAGAAGTTGGATATTGTGGAAGATAGTATTTATA TAGCTTATGGACCCAATGGGAAAGCAACTGGTGAAGGCTTTGTAGAATTTAGGAATGATGCTGACTATAA GGCTGCTCTCTGTCGTCATAAACAATACATGGGCAATCGCTTTATTCAAGTTCATCCAATAACTAAGAAA GGTATGCTTGAAAAGATAGATATGATTCGAAAACGACTTCAGAACTTCAGCTATGACCAGAGGGAACTGG TGTTAAATCCAGAGGGGGAAGTCAGTTCTGCCAAAGTCTGTGCCCATATAACAAACATTCCATTCAGCAT CACCAAGATGGATGTTCTTCAGTTCCTAGAAGGAATCCCAGTGGATGAGAGTGCTGTGCATGTTCTTGTT GATAACAATGGGCAAGGTCTAGGGCAAGCATTGGTTCAGTTTAAAACTGAAGATGACGCACATAAATCTG AACACTTACACCGTAAAAAGCTGAATGGGAGAGAAGCTTTTGTTCATATAGTTACCCTAGAAGATATGAG AGAGATTGAGAAAAATCCCCCAGCCCAAGGAAAAAAGGGTCTAAAGATATCTGTTCCAGGTAATCCTGCA GTACCAGTGATACCTAGTGCAGGGATGCCTGCTGCTGGAATTCCCACTGCCGGGATACCAGGGGCTGGAT TGCCTAGTGCTGGAATGCCTGGTGCTGGGATGCCCAGCTCTGGAATGCCTGGTCCTGGAATGCCCGGTCC CGGAATACCCGGTGCGGGAATACCTGGTCCCGCAATGCCTGGTCCTGCAATGCCTGGTCCCGCAATGCCT GGTCCCGCAATGCCCGGTCCCGCAATGCCCGGTCCCGCAATGCCCGGTCCCGCAATGCCCGGTCCCGCAA TGCCCGGTCCCGCAATACCTGGTCCCGCAATACCTGGTCCCGCAATACCTGGTGCTGGCATCCCCAGTGC AGGAGGAGAAGAGCATGTCTTCTTGACTGTAGGATCAAAGGAGGCCAATAATGGGCCACCATTTAACTTC CCTGGTAATTTTGGTGGGCCCAATGCCTTTGGGCCACCACTCCCCCCTCCAGGATTAGGGGGTGCCTTTG GTGATGTTAGGCCTGTTATGCCTTCGGTTGGAAATAGTGGTTTGCCTGGCCTAGGACTGGAAGTTCCAGG TTTTGGAGGTGCACCAAATAATATAAGTGGGCCATCAGGATTTGGGGGGATCCCTCAGAACTTTGGAAAT GGCCCAGGTAGTTTAAATGCTCCTCCTGGTTTTGGAAGTGGACCCCCTGGTCTTGGAAGTGTTCCTGGGC ATTTGAGTGGGCCTCCAGCCTTTGGGCCTGGGCCTGGGCCTGGCCCAATCCATATTGGGGGTCCCCCTGG CTTTGGAGCTAGCTCTGGAAAACCAGGACCTACAATAATTAAGGTGCAGAACATGCCTTTTACTGTATCT ATTGATGAAATTTTAGATTTCTTTTATGGTTATCAAGTAATTCCAGGTTCAGTATGTTTAAAATACAATG AAAAAGGTATGCCTACAGGTGAAGCCATGGTGGCCTTTGAATCTCGGGATGAAGCCACAGCTGCTGTCAT TGACTTAAATGATAGGCCTATAGGTTCAAGAAAGGTAAAACTTGTTTTAGGGTAGCCGTTCACATCAGTT CCTTATGGGGTATATCTTTATTTTGCTGTGATTAATGCATCCAGATTGTTTTCCTAGTAGTTCCAGGTTA GAACCTGTGGATTGTTTCAATTGCAGATAGATTGGTTTCTATAACACAGAGCATTGGTTGGCTGTTTTAC AACTCACAGAATAAACATTGCAATTTGTTACAGTAAAGCTATCTGGAGAGAACACAAAAATAATTTTGGC ATACAGTAGAGACCATTTGTAATTCTCGATGATCAAGGAATCTAGATTGGTTTTCTTTTATACCACATGG GATGAAGAAAATTGAAACGTATAAATAGAAACGTGAGTAGAGCTCGTGGAGGGGGCTCATGCCAGTGATG GCATTGGAAGTTGGCTACTTTTGCCATTTGTTTTAAAGCTGGACATACTTGCTTTGTTATAGGGTCAAAG CTTTGTCTGAAACCTTCATTTTCTTTTAAAATTGAATAAAGTCTCTGTATCCAGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6833669 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6833669 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13425 same embryo
	EMAGE:13424 same embryo
	EMAGE:13427 same embryo
	EMAGE:13422 same embryo
	EMAGE:13426 same embryo
	EurExpress:euxassay_017452 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source