EMAGE mouse gene expression spatial database

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EMAGE:13425

Atg4c autophagy-related 4C (yeast) ( MGI:2651854)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017446. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017446_01	euxassay_017446_02	euxassay_017446_03	euxassay_017446_04

euxassay_017446_05	euxassay_017446_06	euxassay_017446_07	euxassay_017446_08	euxassay_017446_09

euxassay_017446_10	euxassay_017446_11	euxassay_017446_12	euxassay_017446_13	euxassay_017446_14

euxassay_017446_15	euxassay_017446_16	euxassay_017446_17	euxassay_017446_18	euxassay_017446_19

euxassay_017446_20	euxassay_017446_21	euxassay_017446_22	euxassay_017446_23	euxassay_017446_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13425_wholemount_strong.wlz

13425_wholemount_moderate.wlz

13425_wholemount_weak.wlz

13425_wholemount_possible.wlz

13425_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13425_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9168
Entity Detected:	Atg4c, autophagy-related 4C (yeast) ( MGI:2651854)
Sequence:	sense strand is shown >T9168 TGCGTGGCGGGTAGTAGCTGAAGTCCAGTAGTTACAGTTACGAGGGACAGCTGTAAATCTAACTTGGGCC TGCCCCTCACAAATGAACTGCTCTAGGCTCTGAGCTTGCAGCCCGGGAGCTTAGGGTCTCCGACAGGGCT TCCTGCAGGACCGCGGCCTGCGGAGGCTTCGGTGTGCTGTGCTACGGTGGCCGCGCGGGGCCGCGGGCCG CACGTCCTGCGGTGCGTACGCGCTGTTCGGAGCGGCTGCTGGAGCCGACGCGCGTCTGGTGTGGGGACTC CTGCGCGGCTGTACAGTTATCCAGTCCCGTGTCGCCGAGGGGTCGGCTCGAGGCTGGCGGAGTGGAGAAG GTTGGAGCTTGGTGTTTGTAGTCTACGAATGGCCGGTTCTGGGGGCGGCCCTGCCCTTGGTGCAGACCCG TGGCCAACCGTCTGGGTTCCTGGAGGGTTTTGGAGCCTCTCTCGATTCTGCTTCCTCCTGAGGGTTATCT TCTGACGACTCCTCCTCGCCCTTTGAACTTATATTGGATTTTCTAAAAGAATGGAATCAAGTGATGGCTT TTTCTCCAGAATTTGAATATGGAGGCTTCAGGAACAGATGAAGTTGACAAGCTAAAAACTAAATTTATAT CTGCTTGGAACAATATGAAATATAGTTGGGTGTTGAAAACAAAGACATATTTTAGTAGAAATTCACCTGT TTTATTGCTTGGAAAATGTTATCATTTTAAATATGAAGATGAAAGCAAGATGTTGCCTGCAAGATCAGGA TGTGCCATTGAAGATCATGTAATTGCAGGGAATGTGGAAGAGTTCCGTAAGGATTTCATTTCTAGATTAT GGCTGACCTACAGGGAAGAATTTCCTCAAATCGAAGCTTCAGCCTTGACGACAGACTGTGGCTGGGGCTG CACGCTGCGGACCGGCCAGATGCTGCTGGCTCAGGGACTCATACTCCATTTTCTCGGTAGAGCTTGGACC TGGCCTGATGCGCTGCACATTGAGAACGCAGACTCTGACTCATGGACTTCCAACACTGTCAAAAAGTTTA CTGCATCGTTTGAAGCTTCACTTTCGGGGGACAGAGAACTCAGAACTCCAGCAGTTTCTCTGAAGGAAAC ATCTGGGAAGTGTCCCGATGACCACGCAGTGCGGAATGAGGCTTATCACAGGAAGATCATCTCTTGGTTT GGAGATTCCCCCGTGGCCGTCTTTGGCTTACATCGTCTGATAGAGTTTGGGAAGAAGTCTGGGAAAAAAG CTGGCGATTGGTATGGACCAGCGGTGGTTGCTCACATTTTAAGAAAAGCAGTTGAAGAAGCAAGGCATCC GGATTTGCAAGGGCTAACTATTTATGTTGCCCAGGACTGTACAGTTTACAATTCTGACGTGATTGATAAG CAGACTGACTCCGTGACAGCTGGAGATGCACGTGACAAGGCTGTCATTATTTTAGTTCCTGTTAGACTCG GTGGAGAAAGGACCAATACCGACTATCTGGAGTTTGTGAAGGGTGTTTTAAGCCTTGAATACTGTGTAGG TATCATTGGCGGCAAACCTAAACAGTCATATTACTTTGCTGGATTTCAAGATGACAGTTTGATTTACATG GATCCTCATTACTGCCAGTCTTTTGTAGATGTCAGCATAAAGGATTTCCCTCTCGAGACATTCCACTGTC CATCTCCTAAAAAGATGTCATTTCGAAAAATGGATCCTAGTTGTACGATAGGATTTTACTGTCGAAATGT CCAGGACTTTGAACGAGCGTCTGAAGAAATCACTAAGATGTTGAAAATTTCCTCTAAGGAGAAATATCCA CTATTTACTTTTGTAAATGGCCATTCCAAAGACTTTGATTTTACATCTACCGCAGCCAGTGAAGAAGACC TTTTCTCAGAAGATGAAAGGAAGAATTTCAAAAGATTTAGCACAGAAGAGTTTGTCCTCCTTTAAAGGGG AAGACATTTGTGACATTTGTGCTTCTGAGAGGCGCTCGAACACAGCAGAGTCAAGGAGCGCAACAGTGAG CTGGAGACTCTAACGGAGCGCGTTCCTCTAACAGCACACATTTGAAAACACCTAGACTATAAAGGTATTT TTCTGAATACCTTTTCTAAATGACTTGATGGCTCTTGCTTTCCAGTGATAAATAACAAGCGATTCTGGTT GCATTCCTGTTTCCCAAAGATGTGCTAATGGAACACTACATCAACTGCAAGCCTTTCGGTCCCCAGCATT TTCTCCCTGAGCCCATTGGTCCCCTGAAGGCTTTATGATACATGTTTCCTCAAAGAGTCAGATCACCAAG TTACTTTTAAAAGCCTCCCTTTGTATTAATGACAGGAGACTGGATCTTCAGAGCTACCCAACCTCCTGCT CTTAATGTAACCAGAGGACTTTCGGGTTTGAGTAACTGGATAGTGAAATGGACACATATAATTATGTAAG CAGGATTTTAAGGCAATACTTACCCATATGATTTTATAGCAGCTACTCTTTATGCATTTTGCGGAGTCTT CTATGAGGAATGAATACAAAACACTTGTTTCCCTCCCCCTCCCTCCCTCCCTTCCTCCTTCCTTTCTTTC TTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCCTTCCTTCCTTCCTTCCTTCCTTCCT TCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCTTTTCCTTCTTTATTTCCTTTCTTCTTCCTGTCAGG ACTGGGTCTGGGGGTTGAACCCAGGGCCTTGTATATGATAGGTATGTGTTCTACCTCTGAGCTGTCCTAA AGTATTGATTTTCTCTACATATTTAAAAGTTAAATTTTCAGTAGTATCTATATTTATTTTAATTAGGTAA AGGAAATTACAGACTTATTGTAGCTTTTGTTTTCAATCTTAAAAGTGAAATTTTATGGAAATCCAATGTA TTACTTAATTTTTAACTCAAATGATGAATACAAGGAATATATGGATGCTTTCTGCTTTTACTGTTTGTGT AAAATGGTGTCATGACATTTAGGAGCTGAGACATGTACAGGTTAAATCTGCCTTAAGTGTGCTGTGAGTT TACTTAAAGACAAACTTGTGATATAAGAGTGTAAGAGCTATTACCAAGCCAGGATTATAAAACAAATACT TTAATTAAAAACATGAAAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827140 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827140 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13424 same embryo
	EMAGE:13423 same embryo
	EMAGE:13427 same embryo
	EMAGE:13422 same embryo
	EMAGE:13426 same embryo
	EurExpress:euxassay_017446 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source