EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13455

Gspt1 G1 to S phase transition 1 ( MGI:1316728)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017576. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017576_01	euxassay_017576_02	euxassay_017576_03	euxassay_017576_04

euxassay_017576_05	euxassay_017576_06	euxassay_017576_07	euxassay_017576_08	euxassay_017576_09

euxassay_017576_10	euxassay_017576_11	euxassay_017576_12	euxassay_017576_13	euxassay_017576_14

euxassay_017576_15	euxassay_017576_16	euxassay_017576_17	euxassay_017576_18	euxassay_017576_19

euxassay_017576_20	euxassay_017576_21	euxassay_017576_22	euxassay_017576_23	euxassay_017576_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13455_wholemount_strong.wlz

13455_wholemount_moderate.wlz

13455_wholemount_weak.wlz

13455_wholemount_possible.wlz

13455_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13455_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7096
Entity Detected:	Gspt1, G1 to S phase transition 1 ( MGI:1316728)
Sequence:	sense strand is shown >T7096 TGGATCCCAGCAGCGGCGGTGGCGGCGGTGGCGGCGGCGGTGGGAGCAGCAGCAGCAGCGACTCTGCGCC CGACTGCTGGGACCAGACAGATATGGAGGCCCCCGGGCCCGGGCCTTGCGCCGGCGGCGGGAGCGGCAGC GGCTCCATGGCCGCGGTGGCCGAGGCCCAGCGTGAGAACCTCAGCGCGGCTTTCAGCCGGCAACTCAACG TCAACGCCAAGCCTTTCGTCCCCAACGTCCACGCCGCTGAATTCGTGCCGTCCTTCCTGCGGGGTCCGGC CCAGCCGCCGCTATCCCCAGCTGGCGCAGCCGGCGGCGACCACGGAGCAGGCAGCGGCGCGGGAGGCCCT TCGGAACCTGTAGAGTCCTCTCAAGACCAGTCGTGTGAAGGTTCAAATTCTACTGTTAGCATGGAACTTT CAGAACCTGTTGTGGAAAATGGAGAGACAGAAATGTCCCCAGAAGAATCATGGGAGCACAAAGAAGAAAT AAGTGAAGCGGAGCCAGGGGGTGGTTCCTCAGGAGATGGAAGGCCACCAGAGGAAAGCACCCAAGAAATG ATGGAGGAGGAGGAAGAAATACCGAAACCTAAGTCAGCGGTGGCACCACCAGGTGCTCCCAAGAAGGAAC ATGTAAATGTAGTGTTCATCGGACATGTTGATGCAGGCAAGTCAACCATTGGAGGACAAATAATGTATTT GACTGGAATGGTCGACAAAAGGACACTTGAGAAGTATGAACGAGAAGCTAAAGAAAAAAACAGAGAAACT TGGTACTTGTCTTGGGCCTTAGACACGAATCAGGAAGAACGAGACAAGGGCAAAACCGTGGAGGTGGGTC GTGCCTATTTTGAAACGGAAAAGAAGCATTTTACAATTCTAGATGCTCCTGGCCATAAGAGTTTTGTCCC AAATATGATTGGTGGTGCCTCTCAAGCTGACTTGGCTGTACTGGTAATCTCAGCCAGGAAAGGAGAGTTT GAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCAAAGACAGCAGGTGTCAAAC ACTTAATTGTGCTTATTAATAAGATGGATGATCCAACGGTAAACTGGAGCAACGAGAGATATGAAGAATG CAAAGAGAAACTAGTACCATTTTTGAAAAAAGTTGGCTTCAATCCCAAAAAGGACATTCATTTTATGCCG TGCTCAGGACTGACAGGAGCGAATCTTAAAGAACAATCAGATTTCTGTCCTTGGTACATTGGCTTACCAT TTATTCCATATCTGGATAATTTGCCAAACTTCAATAGATCAGTTGATGGACCAATCAGGCTGCCAATTGT CGATAAGTACAAGGATATGGGGACTGTGGTCCTGGGAAAGCTGGAATCTGGATCTATTTGTAAAGGCCAG CAGCTTGTAATGATGCCAAATAAGCACAACGTGGAAGTTCTTGGAATACTTTCTGATGATGTAGAAACGG ACTCTGTAGCTCCAGGTGAAAACCTCAAAATCAGACTGAAAGGAATTGAAGAGGAAGAGATTCTTCCAGG ATTCATCCTTTGTGATCTTAATAATCTTTGTCATTCTGGACGCACATTTGATGCTCAGATAGTGATTATA GAGCACAAGTCCATCATCTGCCCAGGGTATAATGCGGTGCTGCATATTCATACCTGTATCGAAGAAGTCG AAATAACAGCCTTAATCTGCTTGGTAGACAAGAAATCAGGAGAGAAAAGTAAGACTCGACCCCGTTTTGT AAAACAAGATCAAGTATGCATTGCGCGTTTAAGGACAGCAGGAACCATCTGCCTGGAGACCTTTAAGGAC TTCCCTCAGATGGGACGTTTTACCTTAAGAGATGAGGGTAAGACCATTGCAATTGGAAAAGTTCTGAAAC TGGTTCCAGAGAAAGACTAAGCATTTTCTTGATGACCCTGCACAGTACTGTGAGGAAAATTGACTGCAAA AGCCTACTTCACACCGCCTTCTCTTACTTTCTGCCCATTGACAAACCTCTCCCCATACTTTGCAAAGAAA ATTCACAGCAAAAGTCCACATTATGTCAGCTTTCTCATATTGAGAGCTCTGCTATGCCACTGTTGAATTT TCCCAAGATTTTGCCCCCCTTTCAAACTTCGCTTCCTTGGACAGATTTGGCAATAGCTTTGTAAGTGATG TGGACATAATTGCCTACAATAATGAAAACCTACAGGAATTTTTTTATTTTTCATTTTCTTCTTAGGCATA GTTAGTCTTTTCCCCCCAGGCAGATGATTCTGAGTGTGGGAGTGTGTCTGCACATGTTAAAAAGACAACT ACCATATTAATAAAATATTCAATTTGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4015544 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4015544 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13451 same embryo
	EMAGE:13454 same embryo
	EMAGE:13453 same embryo
	EMAGE:13452 same embryo
	EurExpress:euxassay_017576 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source