EMAGE mouse gene expression spatial database

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EMAGE:13505

Fasl Fas ligand (TNF superfamily, member 6) ( MGI:99255)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017572. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017572_01	euxassay_017572_02	euxassay_017572_03	euxassay_017572_04

euxassay_017572_05	euxassay_017572_06	euxassay_017572_07	euxassay_017572_08	euxassay_017572_09

euxassay_017572_10	euxassay_017572_11	euxassay_017572_12	euxassay_017572_13	euxassay_017572_14

euxassay_017572_15	euxassay_017572_16	euxassay_017572_17	euxassay_017572_18	euxassay_017572_19

euxassay_017572_20	euxassay_017572_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13505_wholemount_strong.wlz

13505_wholemount_moderate.wlz

13505_wholemount_weak.wlz

13505_wholemount_possible.wlz

13505_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13505_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8086
Entity Detected:	Fasl, Fas ligand (TNF superfamily, member 6) ( MGI:99255)
Sequence:	sense strand is shown >T8086 AAGAGGTAATCCCTTGTGATCAACGAAACTGGGTTGTACTTCGTGTATTCCAAAGTATACTTCCGGGGTC AGTCTTGCAACAACCAGCCCCTAAACCACAAGGTCTATATGAGGAACTCTAAGTATCCTGAGGATCTGGT GCTAATGGAGGAGAAGAGGACAGGAGAAAGGTGTTTCCCTTGACTGCGGAAACTTTATAAAGAAAACTTA GCTTCTCTGGAGCAGTCAGCGTCAGAGTTCTGTCCTTGACACCTGAGTCTCCTCCACAAGGCTGTGAGAA GGAAACCCTTTCCTGGGGCTGGGTGCCATGCAGCAGCCCATGAATTACCCATGTCCCCAGATCTTCTGGG TAGACAGCAGTGCCACTTCATCTTGGGCTCCTCCAGGGTCAGTTTTTCCCTGTCCATCTTGTGGGCCTAG AGGGCCGGACCAAAGGAGACCGCCACCTCCACCACCACCTGTGTCACCACTACCACCGCCATCACAACCA CTCCCACTGCCGCCACTGACCCCTCTAAAGAAGAAGGACCACAACACAAATCTGTGGCTACCGGTGGTAT TTTTCATGGTTCTGGTGGCTCTGGTTGGAATGGGATTAGGAATGTATCAGCTCTTCCACCTGCAGAAGGA ACTGGCAGAACTCCGTGAGTTCACCAACCAAAGCCTTAAAGTATCATCTTTTGAAAAGCAAATAGCCAAC CCCAGTACACCCTCTGAAAAAAAAGAGCCGAGGAGTGTGGCCCATTTAACAGGGAACCCCCACTCAAGGT CCATCCCTCTGGAATGGGAAGACACATATGGAACCGCTCTGATCTCTGGAGTGAAGTATAAGAAAGGTGG CCTTGTGATCAACGAAACTGGGTTGTACTTCGTGTATTCCAAAGTATACTTCCGGGGTCAGTCTTGCAAC AACCAGCCCCTAAACCACAAGGTCTATATGAGGAACTCTAAGTATCCTGAGGATCTGGTGCTAATGGAGG AGAAGAGGTTGAACTACTGCACTACTGGACAGATATGGGCCCACAGCAGCCACCTGGGGGCAGTATTCAA TCTTACCAGTGCTGACCATTTATATGTCAACATATCTCAACTCTCTCTGATCAATTTTGAGGAATCTAAG ACCTTTTTCGGCTTGTATAAGCTTTAAAAGAAAAAGCATTTTAAAATGATCTACTATTCTTTATCATGGG CACCAGGAATATTGTCTTGAATGAGAGTCTTCTTAAGACCTATTGAGATTAATTAAGACTACATGAGCCA CAAAGACCTCATGACCGCAAGGTCCAACAGGTCAGCTATCCTTCATTTTCTCGAGGTCCATGGAGTGGTC CTTAATGCCTGCATCATGAGCCAGATGGAAGGAGGTCTGTGACTGAGGGACATAAAGCTTTGGGCTGCTG TGTGACAATGCAGAGGCACAGAGAAAGAACTGTCTGATGTTAAATGGCCAAGAGAATTTTAACCATTGAA GAAGACACCTTTACACTCACTTCCAGGGTGGGTCTACTTACTACCTCACAGAGGCCGTTTTTGAGACATA GTTGTGGTATGAATATACAAGGGTGAGAAAGGAGGCTCATTTGACTGATAAGCTAGAGACTGAAAAAAAG ACAGTGTCTCATTGGCACCATCTTTACTGTTACCTAATGTTTTCTGAGCCGACCTTTGATCCTAACGGAG AAGTAAGAGGGATGTTTGAGGCACAAATCATTCTCTACATAGCATGCATACCTCCAGTGCAATGATGTCT GTGTGTTTGTATGTATGAGAGCAAACAGATTCTAAGGAGTCATATAAATAAAATATGTACATTATGGAGT ACATATTAGAAACCTGTTACATTTGATGCTAGATATCTGAATGTTTCTTGGCAATAAACTCTAATAGTCT TCAAGAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30073084 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30073084 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13507 same embryo
	EMAGE:13506 same embryo
	EMAGE:13504 same embryo
	EMAGE:13503 same embryo
	EMAGE:13502 same embryo
	EurExpress:euxassay_017572 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source