EMAGE mouse gene expression spatial database

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EMAGE:13801

Banf1 barrier to autointegration factor 1 ( MGI:1346330)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_019258_01	euxassay_019258_02	euxassay_019258_03	euxassay_019258_04	euxassay_019258_05

euxassay_019258_06	euxassay_019258_07	euxassay_019258_08	euxassay_019258_09	euxassay_019258_10

euxassay_019258_11	euxassay_019258_12	euxassay_019258_13	euxassay_019258_14	euxassay_019258_15

euxassay_019258_16	euxassay_019258_17	euxassay_019258_18	euxassay_019258_19	euxassay_019258_20

euxassay_019258_21	euxassay_019258_22	euxassay_019258_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13801_wholemount_strong.wlz

13801_wholemount_moderate.wlz

13801_wholemount_weak.wlz

13801_wholemount_possible.wlz

13801_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13801_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T548
Entity Detected:	Banf1, barrier to autointegration factor 1 ( MGI:1346330)
Sequence:	sense strand is shown >T548 TCCTCGAGNCTGTTGGCCTACTGGAGCGGCCGAGTGGCGCGAGGAGCCGTTACCAGAGCCGGAGCCTCCG GCGGGGGGGATTCCCAGGTTAAGCCTGATCACGATGACAACCTCCCAAAAGCACCGAGACTTCGTGGCAG AGCCCATGGGGGAAAAGCCAGTGGGGAGCCTGGCCGGGATTGGTGACGTCCTGAGCAAGAGGCTGGAGGA AAGGGGCTTTGACAAGGCTTATGTGGTCCTTGGCCAGTTTCTGGTGCTAAAGAAAGATGAAGACCTCTTC CGAGAATGGCTGAAGGATACATGTGGTGCCAATGCCAAGCAGTCCCGGGACTGCTTTGGGTGCCTTCGAG AATGGTGTGATGCCTTCTTGTAGTGTTCTCTGGGGATCCCTCATACCCCAGCCACAGCACAGTTCCCAGA GTCTGCAGCAGGGCAGGGCTCCTCCCTGTCCTCCATAAAGGAAAAGATGGCTGTTGTCACCCTCACCTCC TATGTACTCCAGGGTCTATGGGGAGTTCTCTCCCCTCAGCGTTTCAATCTTTCTGGGATCG
Notes:	The probe template was PCR amplified from IMAGE:1499061 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1499061 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13799 same embryo
	EMAGE:13798 same embryo
	EMAGE:13800 same embryo
	EurExpress:euxassay_019258 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source