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EMAGE:13809

Arid1a AT rich interactive domain 1A (SWI-like) ( MGI:1935147)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809
"Pseudo-wholemount" of euxassay_019588. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019588_01 euxassay_019588_02 euxassay_019588_03 euxassay_019588_04
EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809
euxassay_019588_05 euxassay_019588_06 euxassay_019588_07 euxassay_019588_08 euxassay_019588_09
EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809
euxassay_019588_10 euxassay_019588_11 euxassay_019588_12 euxassay_019588_13 euxassay_019588_14
EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809
euxassay_019588_15 euxassay_019588_16 euxassay_019588_17 euxassay_019588_18 euxassay_019588_19
EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809 EMAGE:13809
euxassay_019588_20 euxassay_019588_21 euxassay_019588_22 euxassay_019588_23 euxassay_019588_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13809Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13809_wholemount_strong.wlz
13809_wholemount_moderate.wlz
13809_wholemount_weak.wlz
13809_wholemount_possible.wlz
13809_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13809_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 13 14 15 17
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 10 18 19 20 21 weak expression: see section 09
thyroid gland
weak weak
regionalweak expression: see section 11 17
vibrissa
moderate moderate
regionalmoderate expression: see section 21 22 23 weak expression: see section 07 08 09 10 20
pharyngo-tympanic tube
weak weak
regionalweak expression: see section 01 02 03 23 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 13 15 17 18 19 weak expression: see section 09 10 11 14 16 20
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 13
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 13 17 18 weak expression: see section 11 12 16
lower jaw molar
moderate moderate
regionalmoderate expression: see section 19 weak expression: see section 07
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13 17 18 weak expression: see section 12 16
upper jaw molar
moderate moderate
regionalmoderate expression: see section 19 weak expression: see section 07 20
metanephros
moderate moderate
regionalmoderate expression: see section 09 10 11 18 19 21 22 23 24 weak expression: see section 20
lung
moderate moderate
regionalmoderate expression: see section 03 05 06 07 09 10 11 13 14 15 17 18 19 20 21 22 23 24 weak expression: see section 04 08 12 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55005
Entity Detected:Arid1a, AT rich interactive domain 1A (SWI-like) ( MGI:1935147)
Sequence:sense strand is shown

>T55005
GCAAAACCACCTCAGAATCCAGTTTACCCTGTGCTGTCCAGCTTCTCCCTTGGGAAAGCCTCTCCTGTTC
TCTCTCCTCCCCACCCTCACTCCCTCACACCTTTCTGTTCCCCATCCTCACCTGCTTCCCTCAGGACCCC
ACCCTATTTGAAAAGACAAAGCTCTGCCTACATAGAAGACTTTTTTATTTTAACCAAAGTTACTGTTGTT
TACAGTGAGTTTGGGGAAAAAAATGGCTTTCCCAGTCCTTGCATCAACGGGATGCCACATTTCATAACTG
TTTTTAATGGTTAAAAAAAAAAAAAAAAAAAGGAAAAAAAATACAAAAAAACCCTGAAGGACAAAGGTGA
CTGCTGAGCTGTGTGGTTTGTCGCTGTCCATTCACAATCTCGCAGGAGCCGAGAAGTTCGCAGTTGTGAG
CAGACCCTGTTCACTGGAGAGGCCTGTGCAGTAGAGTGTAGATCCTTTCATGTACTGTACTGTACACCTG
ATACTGTAAACATACTGTAATAATAATGTCTCACATGGAAACGAGAGAAGACGCTGGGTCAGCAGCAAGC
TGTAGTTTTTAAAAATGTTTTTAGTTAAATGTTGAGGAGAAAAAAAATGGCTTTCCCCCCAAAGTATCCT
GTGTGAACCTACAACGCCCTGACCTCTTTCTCTCCTCCTTGATTGTATGAATAGCCCTGAGATCACCTCT
TAGACCTGGTTTTAACCTTTAGCTGCAGCGGCTGCGCTGCCACGTGTGTATATATATGATGTTGTACATT
GCACATACCCTTGAATCTCCACAGTTTGGTCCCCTTCCCAGCTACCCCTTTATAGTATGGCGAGTTAACA
AGTTGGTGACCTGCACAAAGCGAGACACAGCTATTTAATCTCTTGCCAGACATTGCCCCTCTTGGTGCAG
TGCTCTACAGGTCTCTGTAAAAAGCCCTTGCTGTCTCAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCAAAACCACCTCAGAATCC; Reverse Primer - name:unspecified, sequence:CTGAGACAGCAAGGGCTTTT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13808 same embryo
 EMAGE:13810 same embryo
 EurExpress:euxassay_019588 same experiment
 MGI:4823233 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS