EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13810

Mybl1 myeloblastosis oncogene-like 1 ( MGI:99925)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019606. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019606_01	euxassay_019606_02	euxassay_019606_03	euxassay_019606_04

euxassay_019606_05	euxassay_019606_06	euxassay_019606_07	euxassay_019606_08	euxassay_019606_09

euxassay_019606_10	euxassay_019606_11	euxassay_019606_12	euxassay_019606_13	euxassay_019606_14

euxassay_019606_15	euxassay_019606_16	euxassay_019606_17	euxassay_019606_18	euxassay_019606_19

euxassay_019606_20	euxassay_019606_21	euxassay_019606_22	euxassay_019606_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13810_wholemount_strong.wlz

13810_wholemount_moderate.wlz

13810_wholemount_weak.wlz

13810_wholemount_possible.wlz

13810_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13810_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 09 10 11 18 19 20

medulla oblongata basal plate ventricular layer

weak

single cell

weak expression: see section 13

pons ventricular layer

weak

single cell

weak expression: see section 08 09 10 14 16

midbrain ventricular layer

weak

single cell

weak expression: see section 08 09 16

neural retina

strong

regional

strong expression: see section 01 02 03 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

vomeronasal organ

strong

regional

strong expression: see section 12

testis

weak

regional

weak expression: see section 05 06 07 08 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55115
Entity Detected:	Mybl1, myeloblastosis oncogene-like 1 ( MGI:99925)
Sequence:	sense strand is shown >T55115 TAGCACTCCTCCAACCATCCTCAGAAAGAAGAAAAGAATTCGAGTGGGTCAGTCTGCAGGCAGTGAGCTT GGCGATGGCTCACTTAGCGAAGTTGGTAATGCAGCACTCAAACACACACCAGTGAAAACACTACCATTTT CTCCTTCTCAGTTTTTTAACACATGTCCTGGAAATGAACAACTTAATATAGAAAACCCTTCCTTTACATC AACCCCAATTTGTGGGCAGAAAGTTCTCATTACAACTCCTCTTCAGAAGGAAGCAACCCCCAAAGATCAA AAAGAAAATGTAGGATTCAGAACTCCTACTATTAGAAGATCTATACTGGGCACCACACCAAGAACTCCTA CTCCTTTTAAGAATGCACTTGCTGCTCAGGAAAAAAAATATGGACCTCTTAAAATTGTGTCCCAGCCACT TGCCTTTTTGGAAGAAGACATTCGAGAAGTTTTAAAAGAAGAAACTGGAACAGATATATTCCTCAAAGAG GAAGATGAACCTGCTTATAAAAGCTGCAAACAGGAGCACTCTGCATCTGTGAAGAAGGTCAGAAAATCCC TTGCCTTAGAGAGCTGGGACAAAGAAGAACCAGGGACTCAACTGCTAACTGAAGACATTTCAGACATGCA GTCAGAAAATATTCTTACAACATCTTTATTAATGATACCATTATTGGAAATACATGACAATAGGTGCAAC TTGACTCCTGAAAAACAAGATATAAATTCAGCCAACAAAACATATACACTTAATAAAAAGAGACCAAACC CTAACCCTTGTAAAGCTGTCAAATTGGAAAAGAGTCTTCAGTCAAATTGTGAATGGGAAACAGTGGTATA TGGGAAGACAGAAGACCAACTTATCATGACTGAACAAGCGAGAAGATATCTGAGTACTTACACAGCTACC AGCAGCACATCAAGAGCTC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TAGCACTCCTCCAACCATCC; Reverse Primer - name:unspecified, sequence:GAGCTCTTGATGTGCTGCTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13809 same embryo
	EMAGE:13808 same embryo
	EurExpress:euxassay_019606 same experiment
	MGI:4826526 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source