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EMAGE:13850

Sp9 trans-acting transcription factor 9 ( MGI:3574660)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850
"Pseudo-wholemount" of euxassay_019628. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019628_01 euxassay_019628_02 euxassay_019628_03 euxassay_019628_04
EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850
euxassay_019628_05 euxassay_019628_06 euxassay_019628_07 euxassay_019628_08 euxassay_019628_09
EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850
euxassay_019628_10 euxassay_019628_11 euxassay_019628_12 euxassay_019628_13 euxassay_019628_14
EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850
euxassay_019628_15 euxassay_019628_16 euxassay_019628_17 euxassay_019628_18 euxassay_019628_19
EMAGE:13850 EMAGE:13850 EMAGE:13850 EMAGE:13850
euxassay_019628_20 euxassay_019628_21 euxassay_019628_22 euxassay_019628_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13850Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13850_wholemount_strong.wlz
13850_wholemount_moderate.wlz
13850_wholemount_weak.wlz
13850_wholemount_possible.wlz
13850_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13850_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55189
Entity Detected:Sp9, trans-acting transcription factor 9 ( MGI:3574660)
Sequence:sense strand is shown

>T55189
CGACCACCTGAGCAAACACATTAAGACGCACAACGGGGGCGGCGGGGGCAAAAAGGGCAGCGACAGTGAC
ACGGACGCCAGCAACCTGGAGACACCCCGTTCCGAGTCCCCGGACCTCATCCTGCACGATTCCGGCGTCA
GTGCAGCCCGGGCGGCTGCGGCGGCGGCAGCGGCGGCGGCGGCAGCGGCAGCGGCGGCTTCGGCGGGAGG
CAAGGAAGCTGCGACCGGTCCCAACGACTCCTAGAGGTTGGCGGAGAGGCGCGAGCGAGCAAGTAGAGAC
ACCCAGAGCGAGCGAGCGAGAGGTTCGGAGGGCGAGCGAGTGGGCGCACGGGAGGGCAGGGGCTCTCAGA
GAAGCCCCTCGGAGCCCAGGCTGGGAGCGAGGTGGAGCAGCGCGGAACCCCGGGCGGTGGCTTGCTGGCG
GCGCCGGAGTTGCCCTCAACCCGTTGCGCCCAGATGAGAATCGATCTCGAGGTCCAGAAACAAAAGCGAG
CCATCCTCCGACATAAGTGCAGGTTCAAAACTACACACACACACACACACACACACACACACACACACCA
CCCCGGAGTCCCACAAACGCAAGAAC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CGACCACCTGAGCAAACAC; Reverse Primer - name:unspecified, sequence:GTTCTTGCGTTTGTGGGACT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13851 same embryo
 EMAGE:13849 same embryo
 EMAGE:13848 same embryo
 EurExpress:euxassay_019628 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS