EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13882

Cdh16 cadherin 16 ( MGI:106671)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007740. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007740_01	euxassay_007740_02	euxassay_007740_03	euxassay_007740_04

euxassay_007740_05	euxassay_007740_06	euxassay_007740_07	euxassay_007740_08	euxassay_007740_09

euxassay_007740_10	euxassay_007740_11	euxassay_007740_12	euxassay_007740_13	euxassay_007740_14

euxassay_007740_15	euxassay_007740_16	euxassay_007740_17	euxassay_007740_18	euxassay_007740_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13882_wholemount_strong.wlz

13882_wholemount_moderate.wlz

13882_wholemount_weak.wlz

13882_wholemount_possible.wlz

13882_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13882_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thyroid gland

strong

regional

strong expression: see section 09 10 11 13

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 weak expression: see section 08

urinary system

strong

regional

strong expression: see section 03 04 05 16 17

kidney calyx

strong

regional

strong expression: see section 04 05 06 07 08 09 13 14 15

ureter

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15

male reproductive system

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11

right lung

strong

regional

strong expression: see section 12 13 14 15 moderate expression: see section 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1371
Entity Detected:	Cdh16, cadherin 16 ( MGI:106671)
Sequence:	sense strand is shown >T1371 CCTCGAGNCTGTTGGCCTACTGGAAATTTGCCAGAGCTCCCTGAAGAAGGATTCCTTTCTCCTGGAAACT GGACCAAGGGAGAGGCTTTGGGCATCTGAAGGTCTGCCTTGACCATGATCTCTGCCCGGCCGTGGCTACT TTACCTCTCTGTTATTCAGGCTTTCACCACTGAGGCCCAGCCTGCAGAAAGCCTGCACACAGAAGTCCCT GAAAACTATGGTGGAAATTTCCCTTTTTACATACTCAAGCTACCACTACCCCTGGGGAGAGATGAAGGCC ACATTGTCCTATCAGGAGACTCAAACACGGCAGATCAAAACACCTTTGCTGTGGACACAGACTCTGGCTT TCTAGTGGCGACAAGGACCCTGGACCGGGAAGAGAAAGCAGAATACCAACTACAGGTCACCTTGGAGTCT GAGGATGGACGTATCTTGTGGGGTCCACAGCTTGTGACTGTGCATGTGAAAGATGAGAATGACCAGGTAC CCCAATTCTCCCAGGCCATCTACAGAGCTCAGCTGAGCCAGGGCACCAGGCCTGGGGTCCCCTTCCTCTT CCTTGAGG
Notes:	The probe template was PCR amplified from IMAGE:2331777 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2331777 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13884 same embryo
	EMAGE:13883 same embryo
	EMAGE:13885 same embryo
	EMAGE:13886 same embryo
	EMAGE:13881 same embryo
	EurExpress:euxassay_007740 same experiment
	MGI:4823765 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source