EMAGE mouse gene expression spatial database

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EMAGE:13883

Letm1 leucine zipper-EF-hand containing transmembrane protein 1 ( MGI:1932557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007739. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007739_01	euxassay_007739_02	euxassay_007739_03	euxassay_007739_04

euxassay_007739_05	euxassay_007739_06	euxassay_007739_07	euxassay_007739_08	euxassay_007739_09

euxassay_007739_10	euxassay_007739_11	euxassay_007739_12	euxassay_007739_13	euxassay_007739_14

euxassay_007739_15	euxassay_007739_16	euxassay_007739_17	euxassay_007739_18	euxassay_007739_19

euxassay_007739_20	euxassay_007739_21	euxassay_007739_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13883_wholemount_strong.wlz

13883_wholemount_moderate.wlz

13883_wholemount_weak.wlz

13883_wholemount_possible.wlz

13883_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13883_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1376
Entity Detected:	Letm1, leucine zipper-EF-hand containing transmembrane protein 1 ( MGI:1932557)
Sequence:	sense strand is shown >T1376 TCCTCGAGACTGTTGGCCTACTGGACGTTTGTGCCGGGACATGGCCGCTGCGGATGCCGGGTCCTTGAGC TGAGCGCTTGCTGCCGGAGCCAACCTCTGCCGTCAACCGTCCGCGCGGGCTGGGCCCAGGCGGCGGGACG GCCAAGATCCGCCGAGGAAGCTGAGGCAGCTATAGAACGCCGCCGCGGCGGGCGCATGGCGTCCATCTTG CTCAGGAGCTGCCGGGGCCGGGGGCCTGCCCGCCTCGCGCCACCTCGGGCCGCCTCCCCGCGGGGTAGTC TGAGGGATCGAGCTTGTCTCAGCTGTACCAGGACCCTGGGGTTGACGAGCCGTGAGAGTGTTCTGTCTCG TTGCTGTACTCCAGCCCACCCTGTGTACCTCTGCTTCAAAGGTGAGCCCCTCAGCTGTTGGACTCAGAGG CCTGAGTGCCAGGGCACCGCAGCAAGAACAACATGGACACCTGCCTCTGCAAGGCTGGTGGTTACGGGAC CTCAGTACCTTCCTGTGCGCGGCTGGCACTCATCATCTCCGCTAGGAGAGGACTCTGTGATAGAGAAGTC CCTT
Notes:	The probe template was PCR amplified from IMAGE:2331891 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2331891 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13884 same embryo
	EMAGE:13882 same embryo
	EMAGE:13885 same embryo
	EMAGE:13886 same embryo
	EMAGE:13881 same embryo
	EurExpress:euxassay_007739 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source