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EMAGE:13884

Mto1 mitochondrial translation optimization 1 homolog (S. cerevisiae) ( MGI:1915541)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884
"Pseudo-wholemount" of euxassay_007216. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007216_01 euxassay_007216_02 euxassay_007216_03 euxassay_007216_04
EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884
euxassay_007216_05 euxassay_007216_06 euxassay_007216_07 euxassay_007216_08 euxassay_007216_09
EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884
euxassay_007216_10 euxassay_007216_11 euxassay_007216_12 euxassay_007216_13 euxassay_007216_14
EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884 EMAGE:13884
euxassay_007216_15 euxassay_007216_16 euxassay_007216_17 euxassay_007216_18 euxassay_007216_19
EMAGE:13884
euxassay_007216_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13884Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13884_wholemount_strong.wlz
13884_wholemount_moderate.wlz
13884_wholemount_weak.wlz
13884_wholemount_possible.wlz
13884_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13884_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1377
Entity Detected:Mto1, mitochondrial translation optimization 1 homolog (S. cerevisiae) ( MGI:1915541)
Sequence:sense strand is shown

>T1377
TCCTCGAGNCTGTTGGCCTACTGGGCTACGCGCTGACTCGGCCAAGATGGCGACGCCCAGCTGGCTTCTA
CTCCGGTGACAGTTCCGGGCGTCTCTAGCTAGCTTCCCTCAACATGTTCCTTCTCCGAGGCCGTGGCCAT
TGGGCCGCAGCGTCCCTCGGCAGACGGCTGCCTTTGCGTCGCCTCCGCAGCGACAGCGCGGCGCCCTGCA
CCCCGCACTTCGACGTGGTAGTTATCGGAGGAGGGCACGCGGGGACCGAGGCGGCCGCCGCCGCCGCTCG
CTGTGGCTCGCGGACCCTGCTTCTCACGCACCGCGTGGACACGATCGGTCAGATGTCCTGTAATCCTTCC
TTTGGTGGCATCGGAAAGGGGCATTTGATGAGAGAAGTGGATGCCTTGGATGGTCTGTGTTCCCGGATCT
GTGACCAGTCTGGTATACATTACAAAGTGCTAAACCGGCGTAAGGGCCCTGCTGTGTGGGGACTGAGAGC
ACAGATTGACCGGAAACTTTATAAACAGAACATGCAGAAAGAAATCCTGAGCACGCCCCTACTCACTGTT
CAGAAGGGAGCTGTAGAAGATCTAGTTCTTGCAGA
Notes:The probe template was PCR amplified from IMAGE:2331896 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2331896 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13883 same embryo
 EMAGE:13882 same embryo
 EMAGE:13885 same embryo
 EMAGE:13886 same embryo
 EMAGE:13881 same embryo
 EurExpress:euxassay_007216 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS