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EMAGE:13889

Dbt dihydrolipoamide branched chain transacylase E2 ( MGI:105386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13889 EMAGE:13889 EMAGE:13889 EMAGE:13889 EMAGE:13889
"Pseudo-wholemount" of euxassay_007754. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007754_01 euxassay_007754_02 euxassay_007754_03 euxassay_007754_04
EMAGE:13889 EMAGE:13889 EMAGE:13889 EMAGE:13889 EMAGE:13889
euxassay_007754_05 euxassay_007754_06 euxassay_007754_07 euxassay_007754_08 euxassay_007754_09
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euxassay_007754_10 euxassay_007754_11 euxassay_007754_12 euxassay_007754_13 euxassay_007754_14
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euxassay_007754_15 euxassay_007754_16 euxassay_007754_17 euxassay_007754_18 euxassay_007754_19
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euxassay_007754_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13889Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13889_wholemount_strong.wlz
13889_wholemount_moderate.wlz
13889_wholemount_weak.wlz
13889_wholemount_possible.wlz
13889_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13889_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1378
Entity Detected:Dbt, dihydrolipoamide branched chain transacylase E2 ( MGI:105386)
Sequence:sense strand is shown

>T1378
TCCTCGAGCCTGTTGGCCTACTGGAGTAAATGGCTGCAGCGCGTGTACTGAGGACCTGGAGCCAGAACGC
TGTCAGACTGACCTGTGTTCGCTATTTTCAGACATTTAATAGCGCCCGTGTTTTGAAGCCAAAATGTGTG
TGCTCTGTGGGTTATCCATTGTTCAAGTACAGTCAGCCACGTCACTCCCTGAGAACAGCGGCCGTGCTCC
AGGGACAGGTTGTTCAGTTCAAACTCTCCGACATCGGAGAAGGAATTCGAGAAGTAACTATTAAAGAATG
GTATGTAAAAGAAGGAGATACGGTGTCTCAGTTTGACAGCATCTGTGAAGTTCAAAGTGACAAGGCCTCT
GTCACCATCACCAGCCGTTACGATGGCGTCATTAAGAGGCTCTATTACAATCTAGATGATATCGCTTATG
TGGGGAAACCACTGATCGACATTGAGACAGAAGCTTTAAAGGATTCAGAGGAAGATGTTGTTGAAACCCC
CGCTGTGTCCCATGATGAGCACACTCACCAAGAGATAAAAGGCCAGAAAACACTAGCAACGCCTGCAGTG
CGTCGGCTGGCAATGGAAAA
Notes:The probe template was PCR amplified from IMAGE:2331907 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2331907 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13887 same embryo
 EMAGE:13888 same embryo
 EurExpress:euxassay_007754 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS