EMAGE mouse gene expression spatial database

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EMAGE:14027

Mcm3 minichromosome maintenance deficient 3 (S. cerevisiae) ( MGI:101845)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001694. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001694_01	euxassay_001694_02	euxassay_001694_03	euxassay_001694_04

euxassay_001694_05	euxassay_001694_06	euxassay_001694_07	euxassay_001694_08	euxassay_001694_09

euxassay_001694_10	euxassay_001694_11	euxassay_001694_12	euxassay_001694_13	euxassay_001694_14

euxassay_001694_15	euxassay_001694_16	euxassay_001694_17	euxassay_001694_18	euxassay_001694_19

euxassay_001694_20	euxassay_001694_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14027_wholemount_strong.wlz

14027_wholemount_moderate.wlz

14027_wholemount_weak.wlz

14027_wholemount_possible.wlz

14027_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14027_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 12 13 17 18

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 21

metencephalon rest of alar plate marginal layer

moderate

regional

moderate expression: see section 02 03 04 16 17 18

metencephalon rest of alar plate ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 16 17 18 19

midbrain ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3060
Entity Detected:	Mcm3, minichromosome maintenance deficient 3 (S. cerevisiae) ( MGI:101845)
Sequence:	sense strand is shown >T3060 TGGCCTCGAGCCAGAATTCGTCGACATCAAGAGAAGACCGATGATTCTCAGGAGACCCAGGACTCCCAGA AAGTGGAACTCAGTGAGCCCAGGCTGAAGGCTTTTAAGGCGGCCCTCTTAGAAGTGTTCCAGGAAGCTCA TGAGCAGTCTGTGGGCATGCTCCACCTCACAGAATCCATCAACCGGAATAGAGAAGAGCCTTTCTCCTCA GAGGAAATCCAGGCTTGCCTGAGCCGGATGCAGGATGATAACCAGGTCATGGTGTCTGAGGGCATCGTCT TCCTTATCTGAAGTTGTTGCTACCAAGTACTGCCCTGTCCCCAGCCTTTGTCTCCCATCCTCCAGTAACT TTTTTAAGGGATGGAAGCTGATGGTGAGATGATCAGGACTTGGTGGAAGCTTTGTGTATGACTTCTGCCT TGGGGCAAGAGCTGGAGGGAGGGCCAGGACTCAGGCAGAAGGCCTGCCTCACAAGCCCAATCCTAAACAT GGGGAGTCTTCATGATTTGAGCTGTGTTTCTGGGCACAGCTGTGTCCTGCGTTTGT
Notes:	The probe template was PCR amplified from IMAGE:1546461 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546461 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14028 same embryo
	EMAGE:14026 same embryo
	EMAGE:14025 same embryo
	EurExpress:euxassay_001694 same experiment
	MGI:4826128 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source