EMAGE mouse gene expression spatial database

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EMAGE:14029

Stom stomatin ( MGI:95403)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005540. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005540_01	euxassay_005540_02	euxassay_005540_03	euxassay_005540_04

euxassay_005540_05	euxassay_005540_06	euxassay_005540_07	euxassay_005540_08	euxassay_005540_09

euxassay_005540_10	euxassay_005540_11	euxassay_005540_12	euxassay_005540_13	euxassay_005540_14

euxassay_005540_15	euxassay_005540_16	euxassay_005540_17	euxassay_005540_18	euxassay_005540_19

euxassay_005540_20	euxassay_005540_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14029_wholemount_strong.wlz

14029_wholemount_moderate.wlz

14029_wholemount_weak.wlz

14029_wholemount_possible.wlz

14029_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14029_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

strong

regional

strong expression: see section 10 11 12

rectum

strong

regional

strong expression: see section 11

midgut

strong

regional

strong expression: see section 10 11 12 13 14

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10

liver right lobe

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 21 moderate expression: see section 19 weak expression: see section 20

bladder

weak

regional

weak expression: see section 11

bladder

weak

regional

weak expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2727
Entity Detected:	Stom, stomatin ( MGI:95403)
Sequence:	sense strand is shown >T2727 GGCCTCGAGGCCAGATTCGGCACGAGGCTGGAAGAATGGTTCCCTGTCACAGTAGTACTGTTGACACCTG CCCTGAGCTGTTCACTGCCAGGCCCACGTGGAGCCCATGACAGACACTCTGTCCCTAAGCCTTGAGCCCA GCAGGGTTTTCTAGAGGTGTATAGAGCACTTCCACCTGGACTCAAGTTGCATTTACATTTTGAGTTTAAA AAGATGTTTTTTTTTTAATCTGTTTATAGAAGTAGCTCACCTTCCACTGAGGACCAGGAAACCCTGTGTC CTGGTAAAACACCTTGGAGACAGGAAACAATGCCCCTGGTTTTCTAAATTTCCTGTCCAGACACCAACTG GCCTTTGCTTCTGTGCCTTGTGAATTAGTAATCCAGTAGGCTAAAACTCAGTCACAGGGAGGGGAGGAGA AGAGCAAGCCTAGAGGAAGGGGTGCTGGTGGGAGGGTTTCCACGCATTCCCCCCTTCAGCTGTGTACAAT AAAGTGCGTGCCCTGTGACCCGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1513184 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1513184 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14031 same embryo
	EMAGE:14032 same embryo
	EMAGE:14030 same embryo
	EurExpress:euxassay_005540 same experiment
	MGI:4828511 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source