EMAGE mouse gene expression spatial database

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EMAGE:14090

Hbp1 high mobility group box transcription factor 1 ( MGI:894659)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001734. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001734_01	euxassay_001734_02	euxassay_001734_03	euxassay_001734_04

euxassay_001734_05	euxassay_001734_06	euxassay_001734_07	euxassay_001734_08	euxassay_001734_09

euxassay_001734_10	euxassay_001734_11	euxassay_001734_12	euxassay_001734_13	euxassay_001734_14

euxassay_001734_15	euxassay_001734_16	euxassay_001734_17	euxassay_001734_18	euxassay_001734_19

euxassay_001734_20	euxassay_001734_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14090_wholemount_strong.wlz

14090_wholemount_moderate.wlz

14090_wholemount_weak.wlz

14090_wholemount_possible.wlz

14090_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14090_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4105
Entity Detected:	Hbp1, high mobility group box transcription factor 1 ( MGI:894659)
Sequence:	sense strand is shown >T4105 GAAAGACTTGGTAATGGCGACGGGTTTGTCGGAGCACCATAACATGGTGTGGGAAGTGAAGACAAATCAG ATGCCTAATGCAGTACAGAAACTCCTGCTGGTGATGGACAAGAGAGCACCAGGGATGAGTGACTCGCTGG AGTTGCTGCAGTGTAATGAGAATTTGCCATCCTCTCCAGGATACAACTCCTGTGATGAGCACATGGAGCT TGATGACCTTCCTGAACTTCAGGCTGTTCAAAGTGACCCTACCCAATCTGCCATATACCAGCTAAGTTCA GATGTATCTCATCAAGAATATCCAAGATCATCTTGGAGCCAGAATACCTCAGATATACCGGAAAATACTC ACCGTGAAGATGAGGTGGACTGGCTAACAGAGTTAGCAAATATTGCCACTAGTCCACAAAGTCCCCTGAT GCAGTGCTCATTTTATAACAGATCATCTCCTGTACACATCATAGCTACTAGCAAAAGTTTACATTCCTAT GCACGCCCACCACCAGTGTCCTCTTCTTCAAAAAGTGGGCCAGCCTTCCCTCATGACCACTGGAAGGAAG AAACACCAGTAAGACATGAAAGGGCAAACAGTGAGTCAGAGTCAGGCATTTTCTGTATGTCCTCCCTGTC AGATGATGATGATCTGGGCTGGTGCAATTCCTGGCCATCCACCATTTGGCACTGCTTTCTGAAAGGCACG CGGCTGTGCTTCCATAAGGAAAGCAATAAGGAATGGCAAGATGTGGAAGACTTTGCTAGAGCCGCCAGCT GTGATAATGAGGAAGAGATCCAAATGGGCACTCACAAGGGCTATGGGTCTGATGGTCTAAAGTTGTTATC CCATGAAGAGAGTGTGTCATTCGGTGAGTCTGTACTGAAGTTGACATTTGATCCTGGTACAGTAGAAGAT GGCTTGCTCACTGTAGAGTGCAAGCTGGACCACCCTTTCTATGTTAAAAATAAAGGTTGGTCGTCATTTT ATCCAAGCTTGACTGTGGTACAGCATGGCATTCCATGTTGTGAAATTCATATTGGTGACGTATGTCTACC TCCTGGACACCCTGATGCCATTAATTTCGATGATTCAGGTGTTTTTGATACATTTAAAAGCTATGACTTC ACACCTATGGACTCTTCTGCGGTCTATGTTTTGAGTAGTATGGCTCGTCAGCGCCGTGCATCTCTGTCTT GTGGAGGAGGACCTGGTACTGGTCAAGAGTTTTCGGGATCTGAATTCAGTAAAAGCTGTGGTTCACCTGG ATCATCACAGCTCTCTTCTAGTTCTTTGTATGCTAAAGCTGTCAAAAGCCACAGCTCAGGGACTGTGAGT GCCACTTCTCCTAATAAGTGCAAAAGACCAATGAATGCCTTCATGCTTTTTGCCAAAAAATACAGAGTTG AATATACTCAGATGTATCCAGGGAAAGATAACAGAGCCATAAGTGTGATCCTTGGTGACAGGTGGAAAAA AATGAAGAACGAAGAAAGGAGGATGTACACATTAGAAGCAAAGGCTTTGGCTGAAGAACAAAAGCGTTTA AATCCTGACTGTTGGAAAAGGAAAAGAACCAACTCAGGCTCTCAACAGCATTAAACCTGATGCTTGTGTT TGTAAGTCTGCATCTGCATAAGTACTGACTCTGGATGGAAAAGCTTGGAAGACCTTGGTCTCACCATTTG TCTACATTTGTGTGACCATAAAATGCTGGGAGCATTGAGTTTTGTAATGATTTAATGGGTGAGGATTGCT TTCTCCATTAGAGCATTAAGCTATAACTATCAACATTGTAAACCAAATTGCCTTATTTTCCTTCCAAACT TCATATATCTATCAGGTAATATAGGCTTGAAAATTGATATCCTGTGGTGCTAAAGTACAGTAGAAAGATG TGTATACGTTTTATTTTAAATTGTACGAAGGGGAATTTAAAAATACGTAACTGCTGTTTATACATCAGCT CCTTACTGCTTATTAATCTGTATTGTACCCACGATATCAGGAGTGAAGCAGACGCTGGCCTTTCCATGAA TAGCCACCACATGGCCCAGCATCTCTGCCAAACACAGTTGCTTCTAGTCTGGCCAGTGCCAAGAGACAAC ATAACATGGCAGGTGGATGGTGTCGGTGTCATTGTCTCAGCTCAAAGAGCCACCTGCTTCAGATACCATG GGCATGTGCTGAGTGGATGCACCAGGGGGCAGCACAGCTCCATGATTCCCAGTGGGGCCCAAGCCAGGCC AAGGTGCAGTCAGTTAATAGCATTGGGACAGTCATTGTAATGGTGCTGTTACCAGTCGACACCATTGTGT TTGACCGCGTTCCTGTCTCCTCAGCCACATACCCTCATCTGTGCTGTCAGTGTGTCTCTGGTGGGGCAGA CTTTGCACTTACTGCAGTGCACCACTTGCACTTTACTCCCTCCGGCTTCTGAGACAGAGCAAGTACTTCA GCAGTCCTGCTGCTCTTGCTCTGAGCTACAATCATGGCTTCTCATGTTACTTACCAAGTGAGAGTTTTGG TTAGTAATCACAGCTGTAAAATTGATTTACACTTGTTAAACATATTGCCCCTCAATTTTGCACACTATAT TCTTGTATATTATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4458743 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4458743 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14094 same embryo
	EMAGE:14093 same embryo
	EMAGE:14092 same embryo
	EMAGE:14091 same embryo
	EurExpress:euxassay_001734 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source