EMAGE mouse gene expression spatial database

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EMAGE:14096

Gad2 glutamic acid decarboxylase 2 ( MGI:95634)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001769. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001769_01	euxassay_001769_02	euxassay_001769_03	euxassay_001769_04

euxassay_001769_05	euxassay_001769_06	euxassay_001769_07	euxassay_001769_08	euxassay_001769_09

euxassay_001769_10	euxassay_001769_11	euxassay_001769_12	euxassay_001769_13	euxassay_001769_14

euxassay_001769_15	euxassay_001769_16	euxassay_001769_17	euxassay_001769_18	euxassay_001769_19

euxassay_001769_20	euxassay_001769_21	euxassay_001769_22	euxassay_001769_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14096_wholemount_strong.wlz

14096_wholemount_moderate.wlz

14096_wholemount_weak.wlz

14096_wholemount_possible.wlz

14096_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14096_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 10 11 13 14 15

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 16 17 moderate expression: see section 06 07 08 09 10 11 13 14 15

lateral ventricle

moderate

regional

moderate expression: see section 09

telencephalon mantle layer

strong

regional

strong expression: see section 16 17 18 19 moderate expression: see section 03 04 05 06 07 08 10 11 12 13 14 15 weak expression: see section 20 21

midbrain mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

dorsal grey horn

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4145
Entity Detected:	Gad2, glutamic acid decarboxylase 2 ( MGI:95634)
Sequence:	sense strand is shown >T4145 GCAGCTTGCCACAGCCACTTGGAGGCGACCAGCGCCAGTCTAGCAGAACCCATGGCGTCTCCTGGCTCCG GCTTTTGGTCCTTCGGATCTGAAGATGGCTCTGCGGATCCTGAGAACCCGGGCACAGCGAGAGCGTGGTG CCAGGTGGCCCAAAAGTTCACGGGCGGCATCGGAAACAAGCTGTGCGCTCTGCTCTATGGAGACTCCGGG AAGCCAGCGGAGGGCGGCGGGAGCGTGACCTCGCGGGCCGCCACCGGGAAGGTCGCCTGCACCTGCGACC AAAAACCCTGCAACTGCCCCAAAGGGGATGTCAACTACGCGTTTCTGCACGCAACAGACCTGCTGCCAGC GTGTGATGGAGAAAGGCCCACTCTGGCATTTCTACAAGATGTTATGAACATTTTGCTTCAGTACGTGGTG AAAAGTTTCGATAGATCAACTAAAGTGATCGATTTCCATTACCCCAATGAGCTTCTTCAAGAGTATAATT GGGAATTGGCAGACCAACCGCAAAATCTGGAGGAAATTTTGACGCATTGCCAAACAACTCTAAAATATGC AATTAAAACAGGGCATCCCCGATATTTTAATCAGCTGTCTACTGGATTGGATATGGTTGGATTAGCAGCA GACTGGTTGACATCAACAGCAAACACTAACATGTTCACCTATGAGATCGCCCCTGTATTTGTGCTGCTAG AATATGTTACACTAAAGAAAATGAGAGAAATCATTGGCTGGCCCGGGGGCTCTGGCGATGGAATCTTTTC TCCTGGTGGCGCCATCTCCAACATGTATGCCATGCTCATTGCCCGCTATAAGATGTTTCCAGAAGTCAAG GAGAAGGGGATGGCAGCAGTGCCCAGGCTCATCGCGTTCACATCAGAGCATAGTCACTTTTCTCTCAAGA AGGGAGCTGCAGCCTTAGGGATTGGAACAGACAGTGTGATTCTGATTAAATGTGACGAGAGAGGGAAAAT GATTCCATCTGATCTTGAAAGAAGAATCCTTGAAGTCAAACAGAAAGGATTTGTTCCTTTCCTGGTGAGT GCCACAGCTGGAACCACCGTGTATGGGGCTTTTGATCCTCTCTTGGCTGTAGCTGACATCTGCAAAAAGT ATAAGATCTGGATGCACGTGGATGCTGCTTGGGGTGGAGGGTTACTGATGTCCCGGAAACACAAGTGGAA GCTGAGTGGAGTAGAGAGGGCCAACTCTGTGACATGGAATCCTCACAAGATGATGGGTGTCCCCTTGCAG TGTTCAGCTCTCCTGGTTAGAGAGGAGGGACTGATGCAGAGCTGCAACCAGATGCATGCTTCCTACCTCT TTCAGCAAGATAAGCACTATGACCTGTCCTATGACACAGGAGACAAGGCCTTGCAGTGTGGACGCCATGT GGATGTCTTTAAATTATGGCTCATGTGGAGAGCAAAGGGGACTACTGGGTTTGAGGCACACATTGATAAG TGTTTGGAGCTAGCAGAGTATTTATACACTATCATAAAAAACCGAGAAGGCTATGAAATGGTGTTTGATG GGAAGCCTCAACACACAAATGTCTGCTTCTGGTTTGTACCTCCTAGTTTGCGCACTCTGGAAGACAATGA AGAGAGAATGAGCCGCCTCTCAAAGGTGGCGCCAGTGATTAAAGCCAGAATGATGGAGTATGGGACCACA ATGGTCAGCTACCAACCCTTAGGGGACAAGGTCAACTTCTTCCGCATGGTCATCTCAAACCCTGCAGCAA CTCACCAAGACATTGACTTCCTTATTGAAGAAATCGAACGCCTCGGACAAGATTTGTAATCACCTCACTC ATCAAACTGTTTCAGTTCTCTAGGTAGACAGCTAAGTTGTCACAAACTGTGTAAATGTATTTGTAGTTTG TTCCAGAATAATTCTATTTCTATATCGTGGTGTCACAGTAGAGTACAGTTTAAAAAAATCAAAACCAGTG GGGTGGTGGTGGCGCATGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTAAGTTCAAG GCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4482097 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4482097 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14098 same embryo
	EMAGE:14095 same embryo
	EMAGE:14097 same embryo
	EurExpress:euxassay_001769 same experiment
	MGI:4824971 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source