EMAGE mouse gene expression spatial database

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EMAGE:14097

Tagap T-cell activation Rho GTPase-activating protein ( MGI:3615484)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001811. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001811_01	euxassay_001811_02	euxassay_001811_03	euxassay_001811_04

euxassay_001811_05	euxassay_001811_06	euxassay_001811_07	euxassay_001811_08	euxassay_001811_09

euxassay_001811_10	euxassay_001811_11	euxassay_001811_12	euxassay_001811_13	euxassay_001811_14

euxassay_001811_15	euxassay_001811_16	euxassay_001811_17	euxassay_001811_18	euxassay_001811_19

euxassay_001811_20	euxassay_001811_21	euxassay_001811_22	euxassay_001811_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14097_wholemount_strong.wlz

14097_wholemount_moderate.wlz

14097_wholemount_weak.wlz

14097_wholemount_possible.wlz

14097_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14097_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4155
Entity Detected:	Tagap, T-cell activation Rho GTPase-activating protein ( MGI:3615484)
Sequence:	sense strand is shown >T4155 CCACGCGTCCGGGACTCCTAGGGTCAGAGTGTCATGTTGTGTCTCCCGTTTTCCACAGTTCGCCAGAGGT AAAAAGAGCTCTGGCTGAAGACCGTTGCTGGATCAAAGCCTCGAGGTAGTGGACCCCCCTCTTGGACTGT CAGTCTCCATTCCAATGAAGCTGATAAGCAGTCTCGATGGTTCAAAAACACTTAATGCTAACAACATGGA GACATTAATTGAATGTCAGTCAGAGGGTGATATCAAAGTGCCTCCCCTGCTGACATCATGTGAGAGTGAA GACAGCATTTGCCAGCTAACTGAAATTAAGAAGAGAAAGAAAGTGCTGTCCTGGCCATCTCTCATGAGAA AGCTCTCTCCTTCATCCGACTTCTCTGGGTCATTGGAACCAGAGCTGAAAGTGTCGCTGTTTGACCAACC CTTGTCGATCATCTGTGGGGAGAACGACACACTTCCCAGACCCATCCAGGACATCCTCACCATCCTCTGC CTTAAAGGTCCTTCAACGGAAGGGATATTCAGGAAAGCTGCCAGCGAGAAAGCCCGCAAGGAGCTGAAGG AGGGGCTTAACTGTGGGGTCTCCGTGAATCTGAAGCAGCTCCCTGTGCACCTCCTAGCTGTGGTCTTCAA GGACTTCCTCCGAGGGATACCCCTGAAGCTACTCTCCTGTGATCTCTTTGAGGACTGGATGGGCGCCCTG GAGAAGCCCACCGAGGAGGACAGAATCGAGGCCCTGAAGCAGGTTGCTGGTGGCCTCCCCCGGCCCAACC TTCTCCTGCTCAGGCACTTGCTCTACGTGCTCCACCTCATCAGCAAGAACGCTGAGGTCAACAAGATGGA CTCCAGCAACCTCGCCATCTGCATCGGGCCTAACATGCTCACTCTGAAGAACGACCAGAGCCTGTCCTTC CAGGCCCAGAAGGACCTGAACAATAAGGTTAAGATCTTGGTGGAATTCCTCATCGACAACTGCTTTGAGA TATTTGGGGAGAACATTCGGACGCGTTCCCGCATCACTTCTGACGACTCCCTGGAACACACTGACAGTTC AGACGTGTCAACTCTGCAGAATGACTCGGCCTATGACAGCAATGACCCAGATGTGGAGCCCACAAGTGGC GCAGCCTCTCCCAACAGGCAACTGGAGGGTCCCACTCCCACAATGGCTGGTCTGGATACCCGGGGCCAAC GGGACACCTGTGAGTCAAGCTCAGAGTCCAGCGTTAGCATGGTAGTCAGGCTGAAAAGCTCCATTGTCCA ACAAGACAGGCGGTTCTCTGAACCCAACATGTCACCCTCACGAGAGTGCCTCGTGGGCCCAACATCCAAG CAAAAGCTAGCAAGGAGTGAGGACAGCTTCACTCTGTCCCAGGACGCCTCCTGTTCTGAAGGCGATGAAG CTGAAGATCCCTTTACAGAGGAAGTCTTCCCAGCAGTGGACAGCAAACCCAAGAGACCTGTGGATTTGAA AATAAAGAATTGGACCCAAGGTTTAGCATCTCCACAGGGACACATAACCAAAGCTTTCTCCAGATCCTCC CCAGGCGAATCTTTGGGCAGCTCACCTGTGCCTTCTCCATCCTGCCCCAAGAGAAACTTCTTCACCAGAC ACCAGAGTTTCACAACAAAGACAGACAAAACCAAACCCCAGAGAGAAATTAGAAAGCACTCCATGTCGTT TTCCTTTGCGTCTCACAAGAAAGTGCTGCCCCGAACCTCCAGCATTGGGTCTGAGAAATCCAAAGACTTT TCTAGAGACCAACTCCAGAAGGACTTGAGGAAAGAGAGCCAACTTTCTGGCAGAATCGTCCAGGAAAATG AGTCAGAAATCCAAAGCCAAACATCTCTGGGCTTCAGCTTGTCTGGCACCTGGGCCCTCTCAGTCGACAA CACGTTCCAGTTAGTGGATATGAGGAAACCAGGAAGCCCACCATCTTACGAAGAGGCCATTTATTACCAG ACATCAGGACTCACAGCCTACGGTGGCCAGACAGTTGGGAGTATGAGGTCAAGAATGTTCAAGCCAAGCA CAGCAGTGCCCCCTGTGCCTTCTCACCATGGAGGTGACCTCAGTGAAGGGACACCTGGTGGACACAGATT GTCTTCTGTGACTGAGCACTGGACACACAGTCAGACTGTCCATGTCTCTATAGAAACTCAGGGGAGATCT GAGCTACACCAGTTGAGGACAGTGTCCGAGTCCATGCAGAAGGCTAAGCTGGACTGTCTTGGGCCACAAC ACAGCCACTTAGTCTTTGAGGCTGACCAACTCTGCTGTGCTAGAGAATCCTACATTTAAGAAAGCCTTGT ACCATGGCTAACTTGTGTGTGAAGATGCGACTGCAGAGAACTGTCTGTTGAGTCACAGATAAGCTCATTC AGAACTCAGTGTGCGGCTCTCCTCTGTGAGGATGGCTCACACGGTGACACCAAATTTTGCCTGTGTGTAA CCGGGTTTGTGCAATATGTAGTAAATGTATAAAATGTATTATTATATGCAAGGTATTAGATGCAAAGGGT GTCTGCGTACATCCCTTCATGCATTCACTTCTGTATGTTGTTTTTTTGTCCTCAGGCACTTGTTTCTATT AAGTAGAAAGAGTTTTTACTTTTCTTAATGTTACAGCAAATGCCATGCCTGTGTTCACATTAGCCGCCAG CTGCATGTAGGTGGAAATGTCTGAGCCTCTGATGCATCTTCTCCTAAGCAGGCTTGAGACGAGTCAGCAT CATAGAAGGGAACAGACTGGAAGACACTGTACTTGGTGTGTGACCCATCAGCCGCACTGGGAGAAGGTCT TTCAGTCATCTAATGGAATGTGGGTGGCGTGGTGGAAATGCATTCTGTTAGTAGTAACTCACGCAGAGTA CTTTGAGCCTCTGCAGCTCAGTCATGTCTTGTGCAGCTGTGTAGTGGTAAGCACTGTTGTATGCATTGGG GAGTCTTGTATAAGACTTCAGTAGAGTTTATTTATTAGTCACAATAAACTGCCCTCCTCCTTTAGACAGT TTATTACTTGCTGATGTTATATCCTTTGCCTCAAATAAAAATCCGCCAAGAACATTAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4483459 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4483459 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14096 same embryo
	EMAGE:14098 same embryo
	EMAGE:14095 same embryo
	EurExpress:euxassay_001811 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source