EMAGE mouse gene expression spatial database

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EMAGE:14114

Cd74 CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) ( MGI:96534)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001797. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001797_01	euxassay_001797_02	euxassay_001797_03	euxassay_001797_04

euxassay_001797_05	euxassay_001797_06	euxassay_001797_07	euxassay_001797_08	euxassay_001797_09

euxassay_001797_10	euxassay_001797_11	euxassay_001797_12	euxassay_001797_13	euxassay_001797_14

euxassay_001797_15	euxassay_001797_16	euxassay_001797_17	euxassay_001797_18	euxassay_001797_19

euxassay_001797_20	euxassay_001797_21	euxassay_001797_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14114_wholemount_strong.wlz

14114_wholemount_moderate.wlz

14114_wholemount_weak.wlz

14114_wholemount_possible.wlz

14114_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14114_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 13 moderate expression: see section 12 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4411
Entity Detected:	Cd74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) ( MGI:96534)
Sequence:	sense strand is shown >T4411 CGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGTCGTGCGGCTACAGCAAGACAAATAGTATTTTTA ATGAAACCATTTAATAACCCTGGTAGCTTTGAGTTGAAGGCATTGGGAAAATGCAATTAAAGAATGGCTG GATGTAAACAGATCAGGGCTGTTTCCTCGCTGACTCCATCCATTGCATTTGCAGGGCTTCATCACTGTAA ATGGTTGCAAGCCGACAAAGCTGCACCTCCTGAAAAAGACGGACAGCCCATCGCGTGAGCTGTAGAGTTT GTGGACGCATTTCTATCGGTGAGTTACTGTAAAATATCTCCAGGTAAAAGTTGTTTTCTAAAATCCCCTT TGGAAGGTGTTTTCAATTAGCAGCAGCAGCAGCAGCAGCAGCAGCGCCTGTGGGAAAAACTAGAGATTAG AGCCATGGATGACCAACGCGACCTCATCTCTAACCATGAGCAATTGCCCATACTGGGCAACCGCCCTAGA GAGCCAGAAAGGTGCAGCCGTGGAGCTCTGTACACCGGTGTTTCTGTCCTGGTGGCTCTGCTCTTGGCTG GGCAGGCCACCACTGCTTACTTCCTGTACCAGCAACAGGGCCGCCTAGACAAGCTGACCATCACCTCCCA GAACCTGCAACTGGAGAGCCTTCGCATGAAGCTTCCGAAATCTGCCAAACCTGTGAGCCAGATGCGGATG GCTACTCCCTTGCTGATGCGTCCAATGTCCATGGATAACATGCTCCTTGGGCCTGTGAAGAACGTTACCA AGTACGGCAACATGACCCAGGACCATGTGATGCATCTGCTCACGAGGTCTGGACCCCTGGAGTACCCGCA GCTGAAGGGGACCTTCCCAGAGAATCTGAAGCATCTTAAGAACTCCATGGATGGCGTGAACTGGAAGATC TTCGAGAGCTGGATGAAGCAGTGGCTCTTGTTTGAGATGAGCAAGAACTCCCTGGAGGAGAAGAAGCCCA CAGAGGCTCCACCTAAAGAGCCACTGGACATGGAAGACCTATCTTCTGGCCTGGGAGTGACCAGGCAGGA ACTGGGTCAAGTCACCCTGTGAAGACAGAGGCCAGCTCTGCACAGCAGCAGCGCCCCCTGCTCTCCTGTG CCTCAGCCCTTCTTATGTTCCCTGATGTCACACCCCACTTCCCGTCTCCCTGCACCCTGGGGCTTGAGAC TGGTGTCTGTTTCATCGTCCCAGGACACGGCAAATGAAGTCAGAACAGAAGGAGGACGCTGGAGGGCCTT GCTGGCTACCGCTATCTAAAGGGAACCCCCATTTCTGACCCATTAGTAGTCTTGAATGTGGGGCTCTGAG ATAAAGGCCCGCAGACAGGGACAAGGGATGCCCTACCCTTAACCTAGGCTGGACACATTTGCTGCCTTCT CCTCAAGGAAGAAGAACCCAAGCCCCTCCTCCCAGTAACCCCTCCTCACATCCTGCCACCCCCCCTCAAG CCCCACCCCCTTTCAGGTTCCTTGCTCAGCCAAGCTTGTCAGCAGCCTGTAGGATCATGGTTCAAGTGAC AATAAAGGAAGAAAGTAGAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4922119 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4922119 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14115 same embryo
	EMAGE:14113 same embryo
	EurExpress:euxassay_001797 same experiment
	MGI:4823742 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source