EMAGE mouse gene expression spatial database

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EMAGE:14122

Camkk1 calcium/calmodulin-dependent protein kinase kinase 1, alpha ( MGI:1891766)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001783. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001783_01	euxassay_001783_02	euxassay_001783_03	euxassay_001783_04

euxassay_001783_05	euxassay_001783_06	euxassay_001783_07	euxassay_001783_08	euxassay_001783_09

euxassay_001783_10	euxassay_001783_11	euxassay_001783_12	euxassay_001783_13	euxassay_001783_14

euxassay_001783_15	euxassay_001783_16	euxassay_001783_17	euxassay_001783_18	euxassay_001783_19

euxassay_001783_20	euxassay_001783_21	euxassay_001783_22	euxassay_001783_23	euxassay_001783_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14122_wholemount_strong.wlz

14122_wholemount_moderate.wlz

14122_wholemount_weak.wlz

14122_wholemount_possible.wlz

14122_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14122_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4428
Entity Detected:	Camkk1, calcium/calmodulin-dependent protein kinase kinase 1, alpha ( MGI:1891766)
Sequence:	sense strand is shown >T4428 CTAGCCCGGCAAGGCGGGCAGGGCGGGGCGCGCACAGCGGGAGCGAGCCTGGTGCCCACCGCAGAGACTG TCGCCAGGTGCCTAGGTTCCCAACAAGGTTCTACAGACTGTCTGCTGACTGGAGTGATGGAGAGTGGCCC AGCCGTCTGCTGCCAAGACCCTCGTGCAGAGCTGGTGGATCGGGTGGCGGCCATCAATGTGGCCCACTTG GAAGAAGCAGATGAGGGTCCAGAGCCTGCCAGGAATGGTGTGGATCCTCCACCCCGGGCCAGAGCTGCCT CCGTGATCCCTGGCAGTGCTTCAAGACCCACTCCAGTGCGCCCCAGCCTCTCGGCTAGAAAGTTCTCCCT GCAGGAACGACCAGCTGGAAGCTGTCTGGGGGCTCAAGTTGGGCCTTACTCTACAGGACCTGCCAGTCAC ATCTCTCCTCGGTCCTGGCGGAGACCCACCATCGAGTCCCACCGTGTGGCCATCTCAGACACAGAGGACT GTGTGCAACTGAACCAGTACAAGCTGCAGAGTGAGATTGGCAAGGGTGCCTATGGTGTGGTGAGGCTGGC CTACAACGAAAGTGAAGACAGACACTATGCAATGAAAGTTCTTTCCAAAAAGAAGTTACTGAAGCAGTAT GGCTTTCCTCGCCGTCCTCCCCCCAGAGGGTCCCAAGCTACCCAGGGAGGGCCAGCCAAACAGCTGCTGC CCCTGGAGCGTGTGTATCAGGAGATTGCCATTCTAAAGAAGCTGGACCACGTGAATGTAGTCAAATTGAT CGAGGTCCTGGATGATCCCGCTGAAGACAACCTCTATTTGGTGTTCGACCTCCTGAGAAAAGGGCCAGTC ATGGAAGTGCCCTGCGACAAGCCCTTCCCAGAGGAGCAAGCTCGCCTCTACCTCCGGGACATCATCCTGG GCCTCGAGTACTTGCACTGCCAGAAGATTGTCCACAGGGACATCAAGCCATCTAACCTACTCCTTGGGGA CGACGGGCATGTGAAGATCGCCGACTTTGGTGTCAGTAACCAGTTTGAGGGGAATGATGCTCAGCTGTCC AGTACGGCAGGGACCCCGGCATTCATGGCCCCGGAGGCCATTTCTGATTCCGGCCAGAGCTTCAGTGGGA AGGCCTTGGATGTATGGGCCACTGGGGTCACGCTATATTGCTTTGTCTATGGGAAGTGCCCGTTCATTGA TGACTACATCCTGACCCTGCACCGTAAGATCAAGAATGAGGCCGTGGTGTTCCCTGAGGAGCCAGAGGTC AGCGAGGACCTCAAAGACCTGATCCTGAGGATGCTAGACAAGAATCCTGAGACAAGAATTGGGGTGTCAG ATATCAAGTTACACCCTTGGGTGACCAAGCATGGAGAGGAGCCCCTCCCTTCAGAGGAGGAGCACTGCAG TGTGGTGGAGGTGACCGAGGAAGAGGTGAAGAACTCAGTCAGGCTCATCCCCAGCTGGACCACTGTGATC CTGGTCAAGTCTATGCTGAGAAAGCGTTCCTTTGGAAACCCATTCGAGCCCCAAGCACGCAGGGAAGAAA GATCCATGTCTGCACCAGGAAGCTTACTGATGAAAGAAGGATGCGGAGAAGGGTGCAAAAGCCCAGAGCT TCCCGGAGTCCAGGAGGATGAGGCTGCGTCCTGAGTCCCTGCATGTGCCCAGGGCCGTCGGCAGCATGCT CATTCCGCACCTCCAGAGGCCCACCGCCCTCATGCAATAGTCGCCCCTGCAGGCAGGGGGCTGGGGACCG CAGCCCCTCTCCTGGCCCCCCTCACCCATTGTGCTGCATGACCTGCGCACAGGCACATCCAGGGACAGGA TTGGAATGTGTGTCATCTGGGGCTTGGGGGTCCCAGTTGGTCCTCTCTGTTCTGAATCATCTTGCAGGGA ATATTAGAAACCCTACCTGACTGGCTGGTGAGGCCCAGGGGAGAAGGCAAGAGATCAAAATGGCAGGTAG AGACCTGGTGGGCTGCTTCTTAGAGAACGCCCATAGGGATTGGTGGCCCGCCCAGCTGGTGCACACAAGG AGGGCGATTGCCTCACCTCTGTGTGGAAGGTGCTGAGTGCTGGCGTACTCAGATCTCTAGCGTGGATGCT ACGGATAATGGCACGTGCCTGGGAGCAGACTCACCTTGGAGAATCCAGATTCCTCTTTCATTGTCTTTTA CCTGAGGGCTGGGGTGGGAGCAGCTGTCGTGGGGAGCATCTTCCTCTGGCTTCAGGTAGTCCCTGGCACA CTGTTTGGCAGTCATCTGGACTTGACATTCATGGACTGTGTTTTATACTCTGCACTTGCCGGAGAAAGGG CTGAAGTGGATGCAGCTCAGAGCATAGGCCAGAGCCCAGAGCAGTGTACTTTGCCTGTCAGCCTCCCTTC CTGTCTTATGGATCTCCGGAATGAAGGGTACAGAATACCAGCAGTGACAAGGAGGGTCATGACTCTCAGA ACTGTGGCCCCGCAGATGTGGAAGTAACTGGGATCCAGGCTCTGAGCCGACGCTGGCTTACTCACCACAT GCCTATGATGAAGGATAAACTGCCCGGGTGGGAGGCAGGTGGCCCTTCTGCCAGTTGCTTCAGTTAAGGG CATCAGACGGTCAGCCACACAGGTCAGAGCTGCACACAATTGGGACACCCATGGACAATGCCCTTCCAGA ACAGATGTCAGCTCTCTGGATTCTGTGGTCATCTGGCTATTCTAATGTTTACGAATAATGCTCATAGGGA TTGGTCCCCAAAGGGAGATGAGCAGGACTCAGGTAGCCCTCTCTGAAAGCCTCCCGGAAGAGATCCTGGC TGCCCACTTGGGTTTCAGAATGCAGGAAGGCTGGACTAGAAAAGAGGCCGGGTAGGAGCACTGGTTCCCA CTGTATCTCTGTGCCCATGGGTGTACATTGCTCCTTCATGTGCACATGCGTGTGCGTGCCCATCTGCTGT ATACAGCACACTTGCATGTCTCATACTGGCACATGCATCTGTAATATAGTTTCTACATCAACTTATGCCT AGGAGCAGCAGGTAGCCCACTGTCAAAGGGCTCCCATTTCAACCCAATTCCTCTGCACTAAGGCAGCCAA TGGAACCCAGGGCTTGAAAACAAACCATCTAATCCTATCTCTAAGAATACTTTTATAGAGTGACTGGGAG GCAAGCTAAATACCAACCAGTAAATAACAAGGAGTCAGGGAGCTTCGTGGATGGGACCCACATGGGAGAA GCTGTCAGTTGGGTGGCTGGCCTCATTGACCCCAGTGGAGCCAGGAAGTCATGCTCACTGTTGCCTGTTG CTGAGAGCTTTGGTTGGCTTTGGTTGTGATTGTAGCACAAGTGAACAAACTCCATAAGAGTGTTTATAAA ACTTAACTTCTTAGGAAGTGAATTACAAACAATAAAAAGCCCTTTGTTGAGTTTAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:4924656 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4924656 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14120 same embryo
	EMAGE:14119 same embryo
	EMAGE:14124 same embryo
	EMAGE:14123 same embryo
	EMAGE:14121 same embryo
	EurExpress:euxassay_001783 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source