EMAGE mouse gene expression spatial database

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EMAGE:14124

9030425E11Rik RIKEN cDNA 9030425E11 gene ( MGI:1918816)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001798. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001798_01	euxassay_001798_02	euxassay_001798_03	euxassay_001798_04

euxassay_001798_05	euxassay_001798_06	euxassay_001798_07	euxassay_001798_08	euxassay_001798_09

euxassay_001798_10	euxassay_001798_11	euxassay_001798_12	euxassay_001798_13	euxassay_001798_14

euxassay_001798_15	euxassay_001798_16	euxassay_001798_17	euxassay_001798_18	euxassay_001798_19

euxassay_001798_20	euxassay_001798_21	euxassay_001798_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14124_wholemount_strong.wlz

14124_wholemount_moderate.wlz

14124_wholemount_weak.wlz

14124_wholemount_possible.wlz

14124_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14124_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 18

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4116
Entity Detected:	9030425E11Rik, RIKEN cDNA 9030425E11 gene ( MGI:1918816)
Sequence:	sense strand is shown >T4116 CGGACGCGTGGGCTCGGTGCCCCCGGAGCGCAGCGCGCAGGGGACCCGGGCGCGGGGCCAGCGCCCGCAC ATGGCTGCAGCCCCCCGCGCGCACCCCAAGGCGCCGCGCCCTGCTCACAGAAGGTCCGTCGGCTGGGCTC GGTCGCCCTGCAGCCAGGCTGCGCTGAGCCGGGAAGTGCCCGTGTCCGGAGATCGGGATGTCCCTCTTCT TCCTCTGGCTAGTATCCTATTATGTTGGAACGCTGGGAACTCACACTGAGATCAAGAGAGTGGCAGAGGA AAAGGTTACCTTGCCCTGTCACCATCAACTGGGGCTTCCCGAGAAAGACACCCTGGACATTGAATGGCTG CTCACCGATAATGAAGGGAACCAAAAAGTGGTTATTACGTATTCCAGCCGTCATGTCTACAATAACTTGA CCGAGGAGCAGAAGGGCCGAGTGGCCTTCGCTTCCAACTTCCTGGCAGGAGATGCTTCCCTGCAGATTGA GCCTCTGAAACCCAGTGATGAAGGCAGATACACCTGCAAGGTGAAGAATTCAGGACGCTATGTCTGGAGC CACGTCATCTTGAAAGTGCTAGTGAGACCATCCAAGCCCAAGTGTGAGCTGGAAGGAGAGCCGACCGAAG GAAGTGACCTGACGCTGCAGTGTGAGTCTGCCTCTGGAACTAAGCCCATTGTGTATTATTGGCAGCGAAT CCGGGAGAAGGAGGGAGAAGATGAACACCTGCCACCCAAATCCAGAATTGATTACAACAACCCTGGCCGA GTGCTGCTGCAGAATCTCACCATGGCCTCCTCTGGGCTTTACCAGTGCACAGCAGGCAACGAGGCTGGAA AGGAGAGCTGTGTGGTACGGGTGACTGTACAGTATGTGCAGAGCATTGGCATGGTGGCAGGAGCAGTGAC AGGCATAGTGGCAGGAGCCCTGCTCATTTTCCTCCTGATATGGCTGCTAATACGAAGGAAAAGCAAAGAC AGATACGAGGAAGAAGACAGACCTAATGAAATCCGAGAAGACGCCGAAGCGCCCCGAGCCCGCCTTGTGA AGCCTAGCTCCTCTTCCTCAGGCTCCCGGAGCTCACGCTCTGGCTCCTCCTCCACCCGCTCCACCGGGAA CAGTGCCTCCAGAAGCCAGCGGACGCTGTCGAGTGAAGCAGCGCCGCAGCAGCCCGGGCTAGCCCCGCAG GCATACAGCCTCATAGGACCGGAAGTGAGAGGTTCTGAACCAAAGAAAGTCCACCATACGACCCTGACCA AAGCAGAAACCACACTCAGCACAACGCCCAGCCAGAGCAAAGCCTTCCAAACTGTCTGACTTAGAGTGGA CTTGACTTGCGCTTGCCCCAAAGTCAGGATCTTAGCCTAGTCACTGGAGCTCGTCCACCAGCCACGCAAG CCCCTCAGCCAGATAACGATCTCACTTAAGTAGCTGCAGAAATGGCACGGACCAGTTCTGATGAGTACCC TCCTTATATAGGATACCAAACAAACACAAGGACGGAGGCTGACCATCTATCTCTAAAGGCACCTCACTGT GCCTTCAGACAGAGTGGAGGGGAGGAGGGGCCCAAGCTTATTTGGTGAAAATAAAGGGAAAGGTGAGGCT GCACACACCTGAAACATCTTACCTAGGATGTTGCAAGTCACCACAGTCAAGAAGAAGCGGGAATCTCGTA GATCAATTTTCTATTCATTTCTGCAAATTTATTGGATTAGTGTGATTATTCAGATAGTCAAAACAGAAGC CCACGCCTTATAATATACCTATCTGCAACATGTACTGGGAGAACTGCATTTAAGAAATTCACATTAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4459717 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4459717 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14122 same embryo
	EMAGE:14120 same embryo
	EMAGE:14119 same embryo
	EMAGE:14123 same embryo
	EMAGE:14121 same embryo
	EurExpress:euxassay_001798 same experiment
	MGI:4822794 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source