EMAGE mouse gene expression spatial database

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EMAGE:14156

1110008F13Rik RIKEN cDNA 1110008F13 gene ( MGI:1914638)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001851. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001851_01	euxassay_001851_02	euxassay_001851_03	euxassay_001851_04

euxassay_001851_05	euxassay_001851_06	euxassay_001851_07	euxassay_001851_08	euxassay_001851_09

euxassay_001851_10	euxassay_001851_11	euxassay_001851_12	euxassay_001851_13	euxassay_001851_14

euxassay_001851_15	euxassay_001851_16	euxassay_001851_17	euxassay_001851_18	euxassay_001851_19

euxassay_001851_20	euxassay_001851_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14156_wholemount_strong.wlz

14156_wholemount_moderate.wlz

14156_wholemount_weak.wlz

14156_wholemount_possible.wlz

14156_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14156_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 06 07 14 15

thymus primordium

moderate

regional

moderate expression: see section 08 weak expression: see section 09 10 11 12

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 14 15 16

esophagus

moderate

regional

moderate expression: see section 09

hindgut

moderate

regional

moderate expression: see section 10 11

rectum

moderate

regional

moderate expression: see section 11

liver lobe

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4938
Entity Detected:	1110008F13Rik, RIKEN cDNA 1110008F13 gene ( MGI:1914638)
Sequence:	sense strand is shown >T4938 GTCCCTCGCTGCCCGGACTACCGCTGCCGCCCCGCGCGCCGAGTCCCGCGGTCCCAGACCCCGACTCCCT TCGGCTTGGCCCGCGCGCCCCACGCCCGGCTCGGGCGGCGCGACGGGAGGATGAGCGGCGGGCGGCGGAA GGAGGAGCCGCCTCAGCCGCAGCTGGCCAACGGGGCCCTCAAGGTGTCCGTGTGGAGCAAGGTGCTGCGG AGCGACGCGGCCTGGGACGACAAGGATGAATTTTTAGATGTGATCTACTGGTTCCGACAGATCATCGCTC TGGTTTTGGGTGTCATTTGGGGCGTTTTGCCCTTGCGAGGCTTCTTGGGAATAGCAGGATTCTGCCTGAT CAATGCAGGAGTTCTGTATCTCTACTTCAGTAACTACCTACAAATCGACGAGGAAGAATATGGGGGCACG TGGGAGCTCACCAAAGAAGGCTTTATGACATCATTTGCCTTGTTCATGGTCATTTGGATCATCTTTTACA CTGCCATCCACTATGACTGATGGCATTTGCTCCCGTGTCTTCTGTCCAGTCCGAAGGACCCTTTTACTAC AGCACAGGTACATGCCAATTGGGGCACTCCTGCCGCGTGGAACCGGGAAGACCCACGTCTCCTGAACTTA GAATCAACGTGTTGGACATCAGTATTTTCCGCAAGGGTTGTGAAACTTTTCAAAGAACCATCCGCCTTTG GGGAAGCATTTCTGAATTTGTCCATTGTCAACTGTTTTTCCTTGGATACCATCATGTAACAGCTGTTTGC CGAGTGGAGCTGCTGTTGAATTTGATGCGCCTCTGGATCCGGATGAAACATTGTCTTCCTTGGTTCTCCG TCAGGTGTACAGTTTTTAAACCATCACTGCATCTCAAGTCTTCTGAAAACACCATGTGTGTGGTCCATGG TACAATACAAGCAGCCATCTAATAAGTCTCCATTGTAGGATGTTTTCAGAAACTTGAATAAACAAATCTT TAACTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5125733 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5125733 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14157 same embryo
	EurExpress:euxassay_001851 same experiment
	MGI:4822589 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source