EMAGE mouse gene expression spatial database

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EMAGE:14185

Cald1 caldesmon 1 ( MGI:88250)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003971. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003971_01	euxassay_003971_02	euxassay_003971_03	euxassay_003971_04

euxassay_003971_05	euxassay_003971_06	euxassay_003971_07	euxassay_003971_08	euxassay_003971_09

euxassay_003971_10	euxassay_003971_11	euxassay_003971_12	euxassay_003971_13	euxassay_003971_14

euxassay_003971_15	euxassay_003971_16	euxassay_003971_17	euxassay_003971_18	euxassay_003971_19

euxassay_003971_20	euxassay_003971_21	euxassay_003971_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14185_wholemount_strong.wlz

14185_wholemount_moderate.wlz

14185_wholemount_weak.wlz

14185_wholemount_possible.wlz

14185_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14185_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4983
Entity Detected:	Cald1, caldesmon 1 ( MGI:88250)
Sequence:	sense strand is shown >T4983 GGGACAGACCCAAGTGTTAACACAAAGGGAAGGCAGGCGCATCCTGCTCAGCGCATTTCGGCCACAGATA ATATGGCCCGTGTGCTGGCCCAGCCCACACTCTGACCTCCAGGCGCCAGGTCCCCTACCTCAGTCACTCC TGCCCAGACCAGCTGCGGACATGCTTAGCGGATCCGGGTCCCAGGGAAGGCGCTGCCTGGCCACACTCTC TCAAATTGCCTATCAGAGAAATGATGATGACGAAGAAGAGGCTGCCAGGGAACGGCGCCGCCGGGCCCGG CAGGAAAGGCTGCGGCAGAAGCAAGAGGAAGAATCCTTGGGACAGGTGACAGACCAGGTGGAGGCCCATG TCCAGAACAGTGTCCCCGATGAAGAGTCTAAGCCAGCCTCCTCAAACACTCAGGTAGAAGGTGACGAGGA GGCTGCCTTACTGGAGCGCCTGGCTAGACGAGAAGAGAGACGTCAAAAACGCCTTCAGGAAGCCTTAGAG CGTCAGAAGGAGTTTGATCCGACCATAACAGATGGCAGTCTCTCAGGCCCAAGCCGAAGAATGCAAAATG ACTCCGCCGAAAATGAGACAGCAGAGGGGGAAGAAAAAAGAGAGAGTCGCTCAGGACGGTATGAGGTGGA AGAAACAGAAGTGGTCATCAAGTCCTACCAGAAGAACAGCTATCAGGATGCTGAAGACAAAAAGAAAGAA GAAAAGGAAGAGGAGGAGCAAGAGAAGCTGAAGGGAGGGAGCCTTGGCGAAAATCAGATCAAAGATGAGA AGATTAAAAAGGACAAAGAGCCCAAAGAAGAAGTCAAGAGCTTCTTGGATAGAAAGAAAGGATTTACAGA AGTGAAGGCGCAGAATGGAGAATTCATGACCCACAAACTTAAACAAACCGAGAATGCTTTCAGCCCCAGC CGTTCAGGAGGCCGGGCCAGTGGGGACAAGGAAGCTGAAGGTGCCCCACAAGTGGAAGCCGGCAAGAGGC TGGAGGAGCTGCGCCGACGCCGCGGGGAGACGGAGAACGAAGAGTTTGAAAAACTCAAACAGAAGCAACA GGAGGCAGCCTTGGAGCTGGAAGAGCTGAAGAAAAAGAGGGAAGAGAGAAGGAAGGTCCTGGAGGAAGAG GAGCAGAGAAGAAAGCAGGAGGAGGCTGATCGAAAAGCTAGGGAGGAGGAAGAGAAGAGGAGGTTAAAGG AAGAGATCGAAAGGAGAAGGGCAGAAGCTGCTGAGAAGCGCCAGAAGATGCCAGAGGATGGCCTGTCGGA GGACAAGAAGCCGTTCAAGTGCTTCACTCCTAAAGGCTCGTCTCTCAAGATAGAGGAACGAGCAGAGTTT TTGAATAAATCTGTGCAGAAAAGTGGTGTCAGATCTACTCACCAAGCAGCAGTAGTCTCCAAGATTGACA GCCGGCTGGAGCAGTACACCAATGCAATCGAGGGAACAAAGGCTTCGAAACCTATGAAGCCAGCAGCATC GGATCTCCCTGTTCCTGCCGAGGGCGTCCGCAATATCAAGAGCATGTGGGAGAAGGGGAGCGTGTTCTCA GCCCCCTCTGCCTCAGGAACACCCAATAAGGAAACTGCTGGCCTGAAGGTGGGAGTTTCCAGCCGCATTA ACGAATGGCTAACTAAATCACCAGATGGCAACAAGTCACCTGCTCCCAAGCCTTCTGACTTGAGGCCAGG AGATGTATCTGGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACTTCCCCCACTAAGGTC TGAAACAATCGGAGAAAGAACCCAACTGCAAGCCATCTCGCTGGGCCAGCTCAGTTGGAGAGGGCTAATC GCTCTGTTTATATTTATGTTTTCCAATATCCCAGTAAATCCATGTATATGCTCACTATATTTAATAACCA CAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTGTTTCTAAAGAAACCATGCTGTG AAGTAAACTCGCTACTGTCATATGAACAGTGACACCAACCACATCGGAAGTCAACAAAAGGAAACTGAGC TTAAGATTATCTGAGGAATGTATGCAGTATCTAGGGGCGAAACGACCCCGTAGGCTTTGTTTTGTCTCAG TACAGACATCATGTTTCTGCACTTTAGACTAAAGCATGAAAGAAATTATCTAAATAGGCAATCAAAACTC TCTGAAAATAATCCACTTCATATCTGATGTAGGGCAGTAATGATTGTCTGTTTTAAAAAAGATGTATTGT TGAGATATTGCTTTTTTTCTATGCTGATTCATACCTAAATTGGCTGATTACTTTAGCTGACAGTGGTACT GACTTTTTTTCAGGTTAGTTGCTTTGTGGATTTCTTTGGTAGTGATAGTAGACTGAATCACATTTAGATA ACCCAACTATGTAATGAATGTGCATACGTGTAAACAGACACACTAATGGTAGATTACTCTTTCATGTGCT AGACTATTATATTTCATAGTATGTCCTTGTAACTAACTGATATCACAGCTTTTGAAGATTAAGGATCACA ATAGTATATTAATATTTCATATTTGCCAACAGAAACATGGAGTTAGGTATAGCTATGTTTTCAATGCCCA ATGACTTATAAGAAAAAAAGTATTGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5148205 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5148205 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14190 same embryo
	EMAGE:14188 same embryo
	EMAGE:14189 same embryo
	EMAGE:14186 same embryo
	EMAGE:14187 same embryo
	EurExpress:euxassay_003971 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source