EMAGE mouse gene expression spatial database

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EMAGE:14188

Fgf13 fibroblast growth factor 13 ( MGI:109178)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003974. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003974_01	euxassay_003974_02	euxassay_003974_03	euxassay_003974_04

euxassay_003974_05	euxassay_003974_06	euxassay_003974_07	euxassay_003974_08	euxassay_003974_09

euxassay_003974_10	euxassay_003974_11	euxassay_003974_12	euxassay_003974_13	euxassay_003974_14

euxassay_003974_15	euxassay_003974_16	euxassay_003974_17	euxassay_003974_18	euxassay_003974_19

euxassay_003974_20	euxassay_003974_21	euxassay_003974_22	euxassay_003974_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14188_wholemount_strong.wlz

14188_wholemount_moderate.wlz

14188_wholemount_weak.wlz

14188_wholemount_possible.wlz

14188_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14188_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4129
Entity Detected:	Fgf13, fibroblast growth factor 13 ( MGI:109178)
Sequence:	sense strand is shown >T4129 CGAAACTCCGACCCGAGCCGCTTGGACTTGCACCGTGTCCTCGGGGCTCAGGGGCCGAGCGCACGCAGTC ACAAGCGCAGCTCTCTGCCTGCCTCCTGCTAGTCTCGCCTGCCATCCCGGCCCGGGGCTGTGGCGTCGGT TCGGACCAAGGCTACCAGAACCCGCGCGGGAGCCCGAGCACCTTCGGAGGAGCTCAGAAGGCATGCTGAG CCCCCTCCGCGACTGAAGCCGGAGTGCCCAGAAGCCCCGGCCAAGCAAGCGCAGGCTAAGGAGACCCAGG CGGCCAAGGCGCGAGCAGCGCAGCAGCGGAGGAGCGAGAGGAGGCGAAGGCAGAGGGGCCCCCGAAGAAG TGGTGGTGGTGGGCGTCGTGGCCATGGCGGCGGCTATCGCCAGCTCGCTCATCCGGCAAAAGAGACAAGC CCGCGAGCGCGAGAAATCCAATGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGAC AAAAACAAGTTAAACGTCTTTTCCCGGGTCAAACTCTTTGGCTCCAAGAAGAGGCGCAGAAGGAGACCAG AGCCTCAGCTTAAGGGTATAGTTACCAAACTATACAGCCGACAAGGCTACCACTTGCAACTGCAGGCAGA TGGAACCATTGATGGCACCAAAGACGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTT CGGGTGGTGGCTATTCAAGGAGTTCAAACCAAGCTGTATTTGGCAATGAACAGCGAGGGATACTTGTACA CCTCGGAACATTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAATTATTACGTGACATACTC ATCAATGATCTACCGTCAGCAGCAATCCGGCCGAGGGTGGTATCTAGGTCTGAACAAAGAAGGAGAGATC ATGAAAGGCAACCATGTGAAGAAGAACAAGCCTGCAGCACATTTTCTGCCCAAACCACTGAAAGTGGCCA TGTACAAGGAGCCATCTCTGCACGATCTCACGGAGTTCTCCCGATCTGGAAGTGGGACCCCGACCAAGAG CAGAAGCGTCTCTGGTGTACTGAATGGAGGCAAATCCATGAGCCACAACGAATCAACGTAGCCAGTGAGG GCAAAACGAGGGCTCTATAACAGAACCTTACCTTCAGGTGCTGTTGAATTCTTCTAGCAGTCCTTCATCC AAAAGTTCAAACTTATCAGTGAGGTTTACCAAACACACAGGCAGACTTCCCTATTCTCTCTGCCGTTATA CCTTGTTATCACCCATATTAAAGCCCCATAATTTATTCTGAGCTTGTGCAAAAGAGTGCCAAGCATTATC AATGAACTAAACCTGGAAGAAGGACTGCCAAATTTTCTCATGATCTCACCTGTACTTTGGGGATAAATAA AACAGCAAAAATATTTAACAGCACAAATGCTGGTCAATTTGCTTCCCAATTAAGAAAAATTAAAAGACCA CAGCTATTCTTAACAAAGCAAAACAACAAAACAAAGTTAACTGCTTAAATGTTTTGTAGGGGCAAACAAA TTCTGTGAACTGTGCTGTTTTCTTGGCTTCGTGTTTTCTATCTCTGCTTGATTCAAATGTACTCTTTACT TTCATATAGTGCAACTTTATGATTTTTGAAAATTAATGGTTCTATTGACTCTGTCACTTAATCCATATTA ACCAAAGGCAGGGTTGAATCTGAATTGGCATCTGAGGTTCAAGGTAACCGTGTTCTTATGGGTTTTCCTC TCTCGCACCCTCTCCTTTCCACCCTCCCTCTTTCTCTCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTC TTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTTTTTTGTTGT CAATTAGAACTGTAGCATTCTGGTCAAGTTCTTGTGCTGTACTTTGTGCGTAGAAATTGAGTTGTATCGT CAATCCCAGTCAGTAAGATAAGTTGAAACAAGATTATCCTCAAGTGTAGATTTCTCTTAATTCCATTCGT GTATCATGTTAATCTATTGTGGCTTCTTGTGTAAAGACAGGAACTGTGGAACAGTGGCATTGTCTTTCGT GTTGTTCAAATAAGAAGTGTCTCACCTGTACATGTACAAGTCCTCCTGTCATTGGATTTGGCACGTGAAC ATTGTGAACAAGGCAATGTTAAGAATGGTTCCCCCTAAACAACATATATTATACCTGCTACTGGAAAAGT GTTTAAGACTTAGCTAGGTTTCCATTTAGATCTTCATATCTGTTGCATGGAAGAAAGTTGGAATCTTGGC ATAGAGTTGCATGATATGTAAGATTTTGTGCATTAATAATTGTTAAAACCTGTGTTCCAAAAGTGGACAT AGCATGTACAGGCAGTTTTCTGCCCTGTGCACAAAAAGTTAAAAAAAAAGTTGTTTAATATTTGTTGTTG TATACCCAAATACGCACCGAATAAACTCTTTATATTCATTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481123 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481123 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14185 same embryo
	EMAGE:14190 same embryo
	EMAGE:14189 same embryo
	EMAGE:14186 same embryo
	EMAGE:14187 same embryo
	EurExpress:euxassay_003974 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source