EMAGE mouse gene expression spatial database

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EMAGE:14190

Dhx32 DEAH (Asp-Glu-Ala-His) box polypeptide 32 ( MGI:2141813)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003972. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003972_01	euxassay_003972_02	euxassay_003972_03	euxassay_003972_04

euxassay_003972_05	euxassay_003972_06	euxassay_003972_07	euxassay_003972_08	euxassay_003972_09

euxassay_003972_10	euxassay_003972_11	euxassay_003972_12	euxassay_003972_13	euxassay_003972_14

euxassay_003972_15	euxassay_003972_16	euxassay_003972_17	euxassay_003972_18	euxassay_003972_19

euxassay_003972_20	euxassay_003972_21	euxassay_003972_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14190_wholemount_strong.wlz

14190_wholemount_moderate.wlz

14190_wholemount_weak.wlz

14190_wholemount_possible.wlz

14190_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14190_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4120
Entity Detected:	Dhx32, DEAH (Asp-Glu-Ala-His) box polypeptide 32 ( MGI:2141813)
Sequence:	sense strand is shown >T4120 GTGAGCCAGCACCGAAGGTAACCCAGCAACGAGGGACACGGGTTTGAGGAATTAACTTGCAGAAATTCCC GACCTGGGCCTGGGAGCCTGGCTGAGTTTGTGAGCGGGCCTCACACCCTCTCTGCAATGGGCTGCTGAAC GGAGGACTGGACTGTGGAATGTGACCAGCATGAGCTCCCTGCGGGAAGAAATGGATGAAGAGGAACTGGA CCATCCGAACGCCTCTCCAGAGAAACGCTATTTCCCCGAGTCCCTAGATTCCAGTGACGGGGACGAGGAA GGGGTCCTGGCCTGCGAGGATCTGGAGCTGAACCCCTTTGACGGCTTACCGTATTCATCGCGATATTACA AACTTTTGAAAGAGAGAGAAGAGCTTCCGATATGGAAAGAAAAGTACTCCTTCATGGAGAGTCTGCTGCA AAACCAGGTTGTGGTGGTGTCAGGGGACTCCAAGTGTGGCAAGAGCTCTCAGGTTCCTCAGTGGTGTGCT GAATATTGCCTTTCCATCCATTACCAGCACGGGGGCGTGATATGCACCCAGGCCCACAAACAGACGGCAG TCCAGCTGGCCCTGCGGGTGGCCGACGAGATGGATGTGAATATCGGGCACGAAGTCGGCTATGTGATTCC TTTTGAGAACTGCTGCACCACTGAAACAATCCTGAGGTACTGTACTGATGACATGCTGCAGAGAGAGATG ATGTCCAACCCTTTCCTGGGTAGCTATGGCGTCATCATCTTGGACGATGTCCACGAGAGAAGCCTGGCGA CTGACGTGCTGCTCGGACTCCTTAAAGATGTGCTGCTGGCAAGACCAGAGCTGAAGCTCATAGTTAACTG CTCGCCTCTCCTCACCAGTAAACTCAGTTCCTACTATGGAGACGTGCCTGTCATAGAAGTGAGAAATAAG CACCCGGTGGAGGTGGTGTACCTTAGTGGGGCTCAGAAGGATTCCTTCGAGTCTGTCATACGCCTTATCT TTGAAATCCACCGCTCGGGTGAGAAGGGTGACGTTGTGGTCTTCCTGGCCTGTGAACAAGATATTGAAAA AACCTATGAACTTGTGTGTCAGGAAGGATCCAACTTAAACCCAGATGTGGGCGACCTGGTGGTCATCCCT TTGTATCCCAAAGAGAAGTGCAGCCTGTTCAGGCCAGTGGACGAAACCGAGAAAAGATGCCAAGTGTATC AGAGACGGGTGGTGCTGACCACCAGCTGCGGGGAGTCTCTGATCTGGAGCCACACGGTCAAATTTGTCAT TGATGTTGGTCTGGAAAGAAGACAGGTATATAACCCCCGGATCAGAGCCAACTCCCTCGTCCTGCAGCCC ATCAGCCAAAGCCAAGCAGAGATCCGGAAGCAGCTCCTCGGGTCTTCTCCCTCAGGAAAACTTTTCTGTC TGTACACTGAAGAATTTGCCTCCAAAGACATGAGGCCCCTTAAGCCAGCGGAAATGCAGGAAGCCAACCT GACTAGCATGGTGCTCTTCATGAAGAGGGTGGACATTGCAGGCCTGGGCCGCTGCGACTTCATGAACAGG CCAGCTCCAGAAAGCCTGATGCAGGCACTGGAAGATTTAGATTACCTGGCAGCCCTGGACAACGATGGGA ATCTCTCTGAGTTTGGAATCATCATGTCTGAGTTTCCTCTGGATCCACAGCTCTCAAAGTCTATCTTAGC ATCCTGTGAATTTGACTGTGTAGACGAAATGCTAACCATCGCTGCCATGGTAACAGCTCCCAGTTGCTTT TTGCACGTGCCTCATGGTGCTGAGGAGGCTGCAGTGACTTGCTGGAAAACGTTCTTGCACCCTGAAGGTG ATCACTTTACGCTCATCAATGTCTACAATGCCTACCAAGATACAGTTCTGAACTCTGCCAATGAACACTG TGTGGAGATGTGGTGTCACGATTGCTTCCTCAGCTGCTCTGCTCTCAGGATGGCAGACGTTATCCGAGCA GAACTCTTAGAGATCATCAAGCGGATCGAGCTTCCCTATGCAGAGCCTGCCTTTGGCTCCAAGGAAAACG GGTTGAACATCAAGAAAGCGCTGCTCTCTGGGTACTTCATGCAGATTGCTCGGGATGTTGACGGGTCAGG TAACTACTTGATGCTTACACATAAGCAGGTTGCTCAGCTGCATCCCTTATCAAGCTATTCCATCACCAAG AAGATGCCCGAGTGGGTCCTCTTCCACCAGTTCAGCATATCTGAGAACAACTACATCAGGGTCGCCTCCG CAGTCTCCCCTGAGCTATTTATGCAGCTGGTACCACAGTACTATTTCAGTAACCTGCCTCCTAGTGAAAG CAAGGACATCCTACAGCAAGCTGCAGGCCACCTACCCACAGAAACTGTGAACAAGGACCAGGATGTGTGT GACAAGTGCCCAGATGCTACTGAGCAGAGATGCACAATCCAGTGACCTCTCCCAACATAGTGCAACACGG ACACTGCAACAGGAGCTGCCGAAGCCAGGGCAGCCCTGGACATGAACACTTCAAGTATGATTTTAAAATA AAAAAATAGTTTTATTAAGTTCTTAAAATTACTATTCCATGCTTTTCTTATTGGAAGTTTTTAACCAATC ATTCATAATTTGACCAAAATAAAGAAAAAAATCATTTTGTAAATGTGTCACAGACACATGGAACAGAACC CTACTTTTTGTAGAGGACTTGAATCTGAATAAAATAATGAGTTTTTCAGTAAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:4460064 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460064 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14185 same embryo
	EMAGE:14188 same embryo
	EMAGE:14189 same embryo
	EMAGE:14186 same embryo
	EMAGE:14187 same embryo
	EurExpress:euxassay_003972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source