EMAGE mouse gene expression spatial database

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EMAGE:14191

Tmem40 transmembrane protein 40 ( MGI:2137870)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004000. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004000_01	euxassay_004000_02	euxassay_004000_03	euxassay_004000_04

euxassay_004000_05	euxassay_004000_06	euxassay_004000_07	euxassay_004000_08	euxassay_004000_09

euxassay_004000_10	euxassay_004000_11	euxassay_004000_12	euxassay_004000_13	euxassay_004000_14

euxassay_004000_15	euxassay_004000_16	euxassay_004000_17	euxassay_004000_18	euxassay_004000_19

euxassay_004000_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14191_wholemount_strong.wlz

14191_wholemount_moderate.wlz

14191_wholemount_weak.wlz

14191_wholemount_possible.wlz

14191_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14191_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4997
Entity Detected:	Tmem40, transmembrane protein 40 ( MGI:2137870)
Sequence:	sense strand is shown >T4997 CTCACATGAGCCCAGGGACTCCAGGGAAAGTCATGGAGGCTTCAGGATCTTCTTCCCAGTCTCAAGACAG TGGTGGAGTCCACAGGGAAACGGAAGATCACTACCAAGAGACAGAGCTCCACAAGCATCATGGGAAGGCC CGGGAGCGATATAAGAGAGACAAGTCTTCCTCTTCATCCTCATCCTCGTCATCTTCTTCCTCCTCTTCCT CCTCATCTTCTTCCTCCTCCTCAGACAGCAGCGATGAGGACCAGCCCTCCAGAGGTCCCAGGAAACACAG AAGGAGGCCCCGCAGGGACAGCTTGCGTGGTGCCGACCATGGGGAACTGGAAGTGCTAAAGGACGAGCTT CAGCTCTGCGGAGGTGCTGCTGGGGAAATGGTGCCTACTGGGGAGTCAGGACTCCGAAGGAGAGGTTCCG GCTCAGCGGAGGGAGAAGTGGAGGCCTCTCAGTTAAGAAGACTGAACATAAAGAAGGATGATGAGTTTTT CCATTTTGTCCTTCTCTGCTTTGCCATCGGAGCCTTGCTGGTGTGTTACCACTACTATGCAGACTGGTCC ATGTCCCTCGGGGTCGGCCTGCTCACCTTTGCTTCTCTCGAGACCATTGGCATCTATTTCGGGCTCGTGT ACCGGATCCACAGCGTGCTTCAGGGCTTCATCCCCCTCCTTCAGAAGTTCCGGCTGCCAGGGTTCAGGAG AACTAACTGAGATCACTGCCTGACAGCAGCTGAGCCAGTCCCCAATGTGACCACTGTAACCACTGCCATG CCTGAGCCCAGAAGACAGAACGACGCGCTGGAAGACACCAGAAGGCCACACAATAAAGAGCTCTTTCCTT CCACGTGATCTGAGTGTCGGCACCAGTGGGCAGGCTCTTGCTCTGCAGATTAACACGCAGTCTCCGTGGG GAAGAGAAGAGGGGATAGCGTCGGGTCAGCACGGAGGAGCTGGGATGCCATTGCTCATAGGTCAACATGA ATCCTGGGTTCTGCAGGCTGGCATCTGCGGGCCTGGGTCTCTCTGGTGTCCTCAGGCCATGCCCAGGAGC TCACAGTTCCTGCAGAGCCTTCTTGGGACAGCCATTCTGAGGCAGGGAGCTACCAGGCTGGGTTCCCAGG GGAACTTGTTGCAGGTAAGACGCTGGGGACCAAAGCTGCGGACCCATCATAGCTGACCAACCCTGTTGCC CTTGGACTCCTAAAATCCAAATAAATTTTCTGTTCCTTGTGCTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5151159 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5151159 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14193 same embryo
	EMAGE:14194 same embryo
	EMAGE:14192 same embryo
	EurExpress:euxassay_004000 same experiment
	MGI:4828803 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source