EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14236

Kcnab1 potassium voltage-gated channel, shaker-related subfamily, beta member 1 ( MGI:109155)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001789. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001789_01	euxassay_001789_02	euxassay_001789_03	euxassay_001789_04

euxassay_001789_05	euxassay_001789_06	euxassay_001789_07	euxassay_001789_08	euxassay_001789_09

euxassay_001789_10	euxassay_001789_11	euxassay_001789_12	euxassay_001789_13	euxassay_001789_14

euxassay_001789_15	euxassay_001789_16	euxassay_001789_17	euxassay_001789_18	euxassay_001789_19

euxassay_001789_20	euxassay_001789_21	euxassay_001789_22	euxassay_001789_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14236_wholemount_strong.wlz

14236_wholemount_moderate.wlz

14236_wholemount_weak.wlz

14236_wholemount_possible.wlz

14236_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14236_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4142
Entity Detected:	Kcnab1, potassium voltage-gated channel, shaker-related subfamily, beta member 1 ( MGI:109155)
Sequence:	sense strand is shown >T4142 CCTCTGAAGGCATCCAGGAAGGGGGGCAAGAGGGGTTAAAAGACAGCCGAAATCCCCCCAACTGCTCACC CTCTGCCGACCGCCTGTGTTGTGGGGTTCTGAGAGGACCTTGCTCTGTCCTGGGAAGCAATGCAAGTCTC CATAGCCTGCACAGAGCACAATTTGAAGAGTCGAAATGGTGAGGACCGACTTCTGAGCAAGCAGAGCTCC AACGCCCCCAATGTGGTGAACGCAGCCCGGGCCAAATTCCGCACTGTCGCTATCATCGCTCGCAGCCTGG GGACCTTCACCCCTCAGCATCACATTTCTCTCAAAGAGTCCACCGCAAAGCAGACTGGCATGAAATATAG GAATCTTGGGAAATCAGGACTCAGAGTTTCATGCTTGGGTCTTGGAACATGGGTGACATTTGGAGGTCAA ATCTCAGATGAGGTTGCTGAACGGCTGATGACAATTGCCTACGAAAGTGGAGTTAATCTCTTCGACACAG CTGAGGTCTATGCTGCTGGGAAGGCTGAGGTGATTCTGGGAAGCATCATCAAGAAGAAAGGCTGGAGGAG GTCCAGCTTGGTCATCACAACCAAACTCTACTGGGGTGGAAAAGCTGAGACAGAAAGGGGACTGTCAAGA AAGCACATCATTGAAGGACTGAAAGGCTCCCTCCAGAGGCTGCAACTGGAATACGTGGATGTGGTCTTTG CAAATCGCCCAGACAGCAACACTCCCATGGAAGAAATCGTTCGAGCCATGACGCACGTGATCAACCAAGG CATGGCCATGTACTGGGGCACCTCGAGGTGGAGCGCGATGGAGATCATGGAAGCCTACTCTGTCGCACGG CAGTTCAACATGATCCCGCCTGTCTGTGAGCAAGCTGAGTACCATCTTTTCCAGAGAGAGAAGGTGGAGG TCCAGCTGCCGGAGCTCTACCATAAAATAGGAGTTGGTGCAATGACATGGTCTCCACTTGCTTGTGGAAT TATTTCAGGAAAATATGGAAATGGGGTGCCAGAAAGTTCTAGAGCTTCACTGAAGTGCTACCAGTGGTTG AAGGAAAGAATCGTAAGTGAAGAAGGGAGAAAACAGCAAAACAAGCTGAAAGACCTCTCTCCAATCGCTG AGCGCCTGGGGTGCACGCTACCTCAGCTGGCTGTGGCGTGGTGCCTGAGAAATGAGGGTGTGAGTTCTGT GCTCCTGGGATCATCCACTCCGGAACAACTCATTGAAAACCTTGGTGCCATTCAGGTCCTCCCTAAGATG ACATCTCACGTGGTGAACGAGATTGATAACATACTGCGCAACAAGCCCTACAGCAAAAAGGACTATAGAT CATAAGGCAATGCATGAGCCACTTAAGCTGCATGGGTAAAGTCATGATGGGTAGGCAGTGCAGAACGGCC TTACCAGCCACTCTTTTCAATCACTTAGCAGTTTGCTGCTCAACCTCTAGTGTCCCAGGATTCTCTGAGG TTTCTGCTGTTGCTACCACTGTGCACCCTGAATTCTGAACACATCTGAAAATTCACAAGCCAGAAAATCC ATTCTATTTTCTTATCTTGGACTGGTCCCCTACCCTGGCACTGGTCTGTGTACCTCATGTTTTTGCAATG GAAAGAATGCTGGGGTAGGGGAATCTATTATCTTCAGAAGAAGGCTGTACATATATATTTTTTTCAAAAG AACAAATTTCACAAGATACAGTATATGACCTGCATAGGAAACAGGATATCCTCACTCTTTAGTCTTGCTC GGGAGAACAAACTGAACTAGATTTTTCCCCCCATATTTCATATTCACACTAGCAAGTTTTATGTCACCTG ATATTCAAATAAAATTTCTAGATATTTGCTTTCACTATCTCTAAAGTTATATTGTTGCCTGGCCCTGTAC AATTTAACTGGGGTGTCTCTCAGGGTTCTAACATATCCATTTGCATAGTTATAAAGGCATGACTATCCCA AGATACATAATAGATACATAATGCTGATTTATAAAAAGGTCACAATGTTATGGAAACAGCTTTAAATAAA ATGTTAAGTGTTCATTTTTTACTTTCTATTAAAGCCTACATTTACTTTCAATTGCTTGTAGGCACAACTT CTGTTGAGGCTAAATATTTGACTTTAAAAATACATACATGTTCAGTTTTGTAATTAAACCTATAAATACC CAGAAAGGCAAGTGTTCAATTTACCATTGGGACATTATCATATAATGCTTGGAATTTTCTGAAGTTATTA ACATGAAAACAAATCATAGGCGATGTTAAGAAAAAGACAAAAAAACATTTTTTTTCTCATTCCTCGGCAC ATGCTAGATGACTTAACTTGCCCAAAGAAAAAGCAAAATATTCAAGAAGCTCCTGGTTCGAATTGTATCA CACCCTACACCATATGCATGAAAGCTCTTTGCATGGAATAAAGGGGCCTAGCTTTGCACTCAGAATCTGA GATACACTTAGCTCTGCCTCTACGGGCTAATTCCCCTCACTGTATCCCACTTGAGAAAAACTGTAAAACT ATATATACTGTGTGGCTATCTGTAAATACGGATAAGACACAAGGGGTTTTTGTTGTTGTTTTGTTTTCTG TAAAGGTGGGGTCATTTTCTAACTCTTATTTGTCTAACTATAGATGGGCAACATTCTACTTCATCTAGTT AGCAAGTTCTCCATCACTTTAAGATTTACACAAGAAATATACGAACTTAAAATCATTTTCGGATATGTAC CACATGGTTAAGTTCTAAATCTTAAACTATCAGTGAAAGATACCAAGAAATATGATTGTGACTACCTAGC TAGCCTCCAGCAGGCAACTTGGGAGTTTCACCCGTGAATATCTGAATAATGATAGAGATGAGCTAACTTT CCATCTATTCACTGTAAATGTAATCCACTGAACTACTGTACGTATAAATGAGTGGAGTGCTGTCTAAATA AAAATCAATTTATTTGCTTAGAGTAATAAAGGCATCTGCAATCTCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4482268 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4482268 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14238 same embryo
	EMAGE:14237 same embryo
	EMAGE:14241 same embryo
	EMAGE:14240 same embryo
	EMAGE:14239 same embryo
	EurExpress:euxassay_001789 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source