EMAGE mouse gene expression spatial database

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EMAGE:14237

Rassf7 Ras association (RalGDS/AF-6) domain family (N-terminal) member 7 ( MGI:1914235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001766. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001766_01	euxassay_001766_02	euxassay_001766_03	euxassay_001766_04

euxassay_001766_05	euxassay_001766_06	euxassay_001766_07	euxassay_001766_08	euxassay_001766_09

euxassay_001766_10	euxassay_001766_11	euxassay_001766_12	euxassay_001766_13	euxassay_001766_14

euxassay_001766_15	euxassay_001766_16	euxassay_001766_17	euxassay_001766_18	euxassay_001766_19

euxassay_001766_20	euxassay_001766_21	euxassay_001766_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14237_wholemount_strong.wlz

14237_wholemount_moderate.wlz

14237_wholemount_weak.wlz

14237_wholemount_possible.wlz

14237_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14237_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 11 13 14

pancreas

weak

regional

weak expression: see section 10 11 12

epidermis

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

esophagus

moderate

regional

moderate expression: see section 12 13

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 13 14

rectum

moderate

regional

moderate expression: see section 13 14

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

bladder

moderate

regional

moderate expression: see section 13 14

kidney calyx

weak

regional

weak expression: see section 09 11 17 18 19

kidney pelvis

weak

regional

weak expression: see section 10

urethra of male

moderate

regional

moderate expression: see section 13 14

lung

weak

spotted

weak expression: see section 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20

trachea

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2657
Entity Detected:	Rassf7, Ras association (RalGDS/AF-6) domain family (N-terminal) member 7 ( MGI:1914235)
Sequence:	sense strand is shown >T2657 TGGCCTCGAGCCAGATTCGGCACGAGGCCGAAGCGCCCAAGTCCCGAAGGCCAGCAAGTACCCAGGACAG GGATGGTCTTGGAGCTTGTGGCCATGGAGCTGAAGGTCTGGGTGGACGGCATCCAGAGGGTGGTCTGTGG GGTCTCAGAACAGACCACCTGCCAAGAAGTGGTCATTGCGCTGGCCCAAGCTATAGGCCAGACAGGTCGA TTTGTCCTTGTGCAGCGTCTTAGGGAGAAGGAACGACAGCTGCTGCCACAGGAGTGTCCAGTGGGAGCCC AGGCCACTTGTGGACAGTTTGCCAATGATGTTCAGTTTGTCCTGAGGCGGACAGGGCCTAGCCTGTCTGG AAGGCCCTCCTCAGACAACTGTCCACCCCCAGAACGATGCCCAGTTCGTGCCAGCCTCCCCCCAAAGCCA TCGGCGATACCAGGCCGTGAGCCACGCAAAGCACTGACCTTCAACCTTAGATGTCCCAAGCTGGTCCCCA GCCCATCTATTCCTGAGCCTGCAGCCCTGGTAGGACCCATACCAGACGGCTTTGCAGACCTGCAGGA
Notes:	The probe template was PCR amplified from IMAGE:1510675 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1510675 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14238 same embryo
	EMAGE:14241 same embryo
	EMAGE:14240 same embryo
	EMAGE:14239 same embryo
	EMAGE:14236 same embryo
	EurExpress:euxassay_001766 same experiment
	MGI:4827638 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source