EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14263

Ostc oligosaccharyltransferase complex subunit ( MGI:1913607)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003969. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003969_01	euxassay_003969_02	euxassay_003969_03	euxassay_003969_04

euxassay_003969_05	euxassay_003969_06	euxassay_003969_07	euxassay_003969_08	euxassay_003969_09

euxassay_003969_10	euxassay_003969_11	euxassay_003969_12	euxassay_003969_13	euxassay_003969_14

euxassay_003969_15	euxassay_003969_16	euxassay_003969_17	euxassay_003969_18	euxassay_003969_19

euxassay_003969_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14263_wholemount_strong.wlz

14263_wholemount_moderate.wlz

14263_wholemount_weak.wlz

14263_wholemount_possible.wlz

14263_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14263_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 06 16 17 18 19

meckel's cartilage

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 03 04 05 06 07 08 09 13 14 15 16

cranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

strong

regional

strong expression: see section 07 08 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4990
Entity Detected:	Ostc, oligosaccharyltransferase complex subunit ( MGI:1913607)
Sequence:	sense strand is shown >T4990 GCGGAAGGCTGGAGGCCAGAGGGCTGGCCCTACCGCCGGGGACATGGAGACTCTGTACCGAGTCCCATTC TTAGTGCTCGAATGCCCCAACCTGAAGCTGAAGAAACCGCCCTGGGTGCACATGCCGTCGGCTATGACGG TGTACGCCCTGGTGGTGGTATCTTACTTCCTCATTACCGGAGGAATAATCTATGATGTTATCGTTGAACC TCCAAGTGTTGGCTCAATGACCGACGAACATGGGCATCAGAGACCAGTAGCTTTCTTGGCTTACAGAGTA AACGGACAGTATATCATGGAAGGGCTTGCGTCCAGCTTTCTCTTTACAATGGGAGGTTTAGGTTTCATAA TCCTGGACCGATCCAATGCACCAAACATACCGAAACTCAACAGGTTTCTTCTTCTGTTCATCGGCTTCGT CTGTGTTCTCCTGAGTTTCTTCATGGCCAGAGTGTTCATGAGAATGAAATTGCCGGGCTACCTGATGGGC TAGTGTCTTTTGAGAGGAAATCAGTGGATCCCGGATTTGCTCCTGTTAATGAAGTTTTAAAGGCTGACCC ACTCCCTCGTGCAATGTGGAAAAAAATGAAGAGCAGTGTAAACGCTGCACTGAGCGACACCCTAGCAAGC ACAGCAGAGCTAGGACCGGAAGTCACGGGATCAGAGACAAGTTCATTACAGTATTTCCCTTGATGACCTA CCCCAGTGTACCAGCTATGTTAAACGGCATTTTTTCTTAGTTTTTTATTTCTTAAAGAAAATATACTCCT CCTAAAACTTTGGTATTGAATAAAGTGATTATTTTTTACAAACCCGTCATCAGCTTTTGGACATGATATT TCTGATTTTCAGAAATTAACGCAAATTGAGAAGTATATTGTGAGAGCTGAGATATTTGGACGACTGGCCA GTTCCTGCAGTGGTGCTTTAAATGGCGTGTGTGCTACTCTGCAATTTCAGATGCATACGGGAGACTTGTT TCCTGAACAACATGATGTATGAAAGGAGCAGAAATAAATGATTTTTCTAATTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5149713 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5149713 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14267 same embryo
	EMAGE:14266 same embryo
	EMAGE:14264 same embryo
	EMAGE:14268 same embryo
	EMAGE:14265 same embryo
	EurExpress:euxassay_003969 same experiment
	MGI:4826999 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source