EMAGE mouse gene expression spatial database

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EMAGE:14268

Serpina1b serine (or cysteine) preptidase inhibitor, clade A, member 1B ( MGI:891970)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003999. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003999_01	euxassay_003999_02	euxassay_003999_03	euxassay_003999_04

euxassay_003999_05	euxassay_003999_06	euxassay_003999_07	euxassay_003999_08	euxassay_003999_09

euxassay_003999_10	euxassay_003999_11	euxassay_003999_12	euxassay_003999_13	euxassay_003999_14

euxassay_003999_15	euxassay_003999_16	euxassay_003999_17	euxassay_003999_18	euxassay_003999_19

euxassay_003999_20	euxassay_003999_21	euxassay_003999_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14268_wholemount_strong.wlz

14268_wholemount_moderate.wlz

14268_wholemount_weak.wlz

14268_wholemount_possible.wlz

14268_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14268_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 09 10 11 12 15 16

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

strong

regional

strong expression: see section 14 15 16 17 18 19 20 22 weak expression: see section 21

kidney calyx

moderate

regional

moderate expression: see section 08 09 19 weak expression: see section 18 20

nucleus pulposus

moderate

regional

moderate expression: see section 12 13

rectum lumen

strong

regional

strong expression: see section 15 16

midgut loop lumen

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4981
Entity Detected:	Serpina1b, serine (or cysteine) preptidase inhibitor, clade A, member 1B ( MGI:891970)
Sequence:	sense strand is shown >T4981 GAAGGGTTAGTCTTAATGTGCTCCTAGCACAGGGCGAGGCCCCTTCCTTCCCTAGCTCTGCCACTCCCAG ATCTATGGCTGCCACCTAAAGCTGATCTCCTGGAGACAGACAATGGAGACTCTTAGCAAACTGGCTCCCT GACACCCTCGGGAGTCAAGAATCTGATCCTGAGGAAGAGTGGGAATCAGCACCGACCAGAGGTTCAGGAG GCATGGCTACCCGGGATCTGGCCAGGATACTCAGGAAAATGACTCCCTCCATCTCATGGGGTCTACTGCT TCTGGCAGGCCTGTGTTGCCTGGTCCCCAGCTTTCTGGCTGAGGATGTTCAGGAGACAGACACCTCCCAG AAGGATCAGTCCCCAGCCTCCCATGAGATCGCTACAAACCTGGGAGACTTTGCAATCAGCCTATACCGGG AGCTGGTCCATCAGTCCAACACTTCCAACATCTTCTTCTCCCCAGTGAGCATTGCCACAGCCTTTGCTAT GCTCTCCCTAGGGAGCAAGGGTGACACTCACACGCAGATCCTAGAGGGCCTGCAGTTCAACCTCACACAA ACATCGGAGGCTGACATCCACAAGTCCTTCCAACACCTCCTCCAAACCCTCAACAGACCAGACAGTGAGC TGCAGTTGAGCACAGGCAATGGCCTCTTTGTCAACAATGACCTGAAGCTGGTGGAGAAGTTTCTGGAAGA GGCCAAGAACCATTATCAGGCAGAAGTCTTCTCTGTCAACTTTGCAGAGTCAGAGGAGGCCAAGAAAGTG ATTAATGATTTTGTGGAGAAGGGAACCCAAGGAAAGATAGTTGAGGCAGTGAAAGAACTGGACCAAGACA CAGTTTTCGCCCTGGCAAATTACATTCTTTTTAAAGGCAAATGGAAGAAGCCATTCGATCCTGAGAACAC TGAAGAAGCTGAGTTCCACGTGGACAAGTCCACCACGGTGAAGGTGCCCATGATGACCCTCTCGGGCATG CTTGATGTGCACCATTGCAGCACACTCTCCAGCTGGGTGCTGCTGATGGATTACGTGGGCAACGCCAGTG CTGTCTTCCTCCTGCCCGAAGATGGGAAGATGCAGCATCTGGAGCAAACTCTCAGCAAGGAGCTCATCTC TAAGATCCTGCTAAACAGGCACAGAAGGTTAGTCCAGATCCATATCCCCAGACTGTCCATCTCTGGAGAC TATAACTTGAAGACACTCATGAGTCCACTGGGCATCACCCGGATCTTCAACAATGGGGCTGACCTCTCCG GAATCACAGAGGAGAATGCTCCCCTGAAGCTCAGCAAGGCTGTGCATAAGGCTGTGCTGACCATCGATGA GACAGGAACAGAAGCTGCAGCAGCCACAGTCTTTGAAGCCGTTCCTATGTCTATGCCCCCTATCCTGCGC TTCGACCACCCTTTCCTTTTTATAATATTTGAAGAACACACTCAGAGCCCCATCTTTGTGGGAAAAGTGG TAGATCCCACACATAAATGACCACCCTACGATGTCATCCTTCCTTCTGAATTGGGTCCCTTCCATTAAAC ACAGGCTGGCCTGGCTCGTGCCTGATGCTACAGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5137448 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5137448 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14263 same embryo
	EMAGE:14267 same embryo
	EMAGE:14266 same embryo
	EMAGE:14264 same embryo
	EMAGE:14265 same embryo
	EurExpress:euxassay_003999 same experiment
	MGI:4827975 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source